LOCUS       sequence-252178-        6913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse CD72a in animal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6913)
  TITLE     Tsubata CD72, SmB, and U1-C plasmids
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMXs-CD72a-IRES-hCD8
FEATURES             Location/Qualifiers
     source          1..6913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             72..488
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     418..440
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     456..472
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    498..872
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     810..826
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(2006..2580,2581..2583,2584..2592)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2173..2190)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2400..2419
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             3390..3859
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3989..4006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4160..4748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4240..4259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4912..5703,5704..5772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5535..5554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5773..5877)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5907..6500
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    join(6563..6913,1..7)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa
       61 atatgggccc gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
      121 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
      181 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
      241 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
      301 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
      361 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
      421 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      481 taggcgcccc catatggcca tatgagatct tatatggggc acccccgccc cttgtaaact
      541 tccctgaccc tgacatgaca agagttacta acagcccctc tctccaagct cacttacagg
      601 ctctctactt agtccagcac gaagtctgga gacctctggc ggcagcctac caagaacaac
      661 tggaccgacc ggtggtacct cacccttacc gagtcggcga cacagtgtgg gtccgccgac
      721 accagactaa gaacctagaa cctcgctgga aaggacctta cacagtcctg ctgaccaccc
      781 ccaccgccct caaagtagac ggcatcgcag cttggataca cgccgcccac gtgaaggctg
      841 ccgaccccgg gggtggacca tcctctagac tgccggatcc cagtgtggtg gtacgggaat
      901 tcatggctga cgctatcacg tatgcagacc tgcgctttgt gaaagtgccc ctgaagaaca
      961 gcgcatctaa ccatctagga caggactgtg aggcctatga agatggggaa ctcacctacg
     1021 agaacgtgca agtgtctcca gtcccaggag ggccaccagg cttggcttcc cctgcactag
     1081 cggacaaagc aggggtcggg tcagagcaac caactgcgac ctggagctct gtgaagtcgt
     1141 ctgctctcag gcagattccc cgctgtccta cggtctgctt gcaaaacttc ttgcttggcc
     1201 ttctcctgtc ctgtctgatg ttaggggtgg ctgtcatctg cctgggagtt cgctatctgc
     1261 aggtgtctca gcagttccag gaggggacca ggatttggga agccaccaat agcagcctgc
     1321 agcagcagct cagggagaag ataagtcagc tggggcagaa ggaggtggag cttcaggagt
     1381 ctcagaaaga gctgatctcg agccaggaca cattacagga gaagcagagg actcacaagg
     1441 acactgagca gcaactacaa gcctgccagg ctgagagagc gaagaccaag gagaacctga
     1501 aaactgagga ggagcggagg agggacctgg accagaggtt gacaagcacg cgggagacac
     1561 tgaggcgctt gtcctcctgt tcatcagaca cctgctgtcc atgcggatgg attccatatc
     1621 aggaaaggtg cttttacatc tcacataccc tcagaagtct ggaggagagc caaaaatact
     1681 gcacatctct gtcctccaaa ctggcagcat tcgatgaacc ttctaagtat tactatgaag
     1741 tttctctgcc cagcggctta gaggagttgc tagatcgttc gaagtcatat tggatacaga
     1801 tgagcaagaa gtggaggcat gactatgact ctcaaagccg atattgtgac aagataaaaa
     1861 aatattacca gaagtggaaa agaacatttt ctgagtgtgc agagcttcac ccctgcattt
     1921 gtgagtcgga ggctttcagg tttcctgatg ggatccatct gaactgaacc ggatagcggc
     1981 cgctacgtaa attccgcccc ccccccccct ctccctcccc cccccctaac gttactggcc
     2041 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     2101 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     2161 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     2221 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     2281 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     2341 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     2401 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     2461 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     2521 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     2581 atggccacaa ccatggcctt accagtgacc gccttgctcc tgccgctggc cttgctgctc
     2641 cacgccgcca ggccgagcca gttccgggtg tcgccgctgg atcggacctg gaacctgggc
     2701 gagacagtgg agctgaagtg ccaggtgctg ctgtccaacc cgacgtcggg ctgctcgtgg
     2761 ctcttccagc cgcgcggcgc cgccgccagt cccaccttcc tcctatacct ctcccaaaac
     2821 aagcccaagg cggccgaggg gctggacacc cagcggttct cgggcaagag gttgggggac
     2881 accttcgtcc tcaccctgag cgacttccgc cgagagaacg agggctacta tttctgctcg
     2941 gccctgagca actccatcat gtacttcagc cacttcgtgc cggtcttcct gccagcgaag
     3001 cccaccacga cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc
     3061 ctgtccctgc gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg
     3121 ctggacttcg cctgtgatat ctacatctgg gcgcccttgg ccgggacttg tggggtcctt
     3181 ctcctgtcac tggttatcac cctttactgc aaccactgag tcgacgataa aataaaagat
     3241 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     3301 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     3361 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     3421 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     3481 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     3541 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     3601 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     3661 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     3721 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     3781 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     3841 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
     3901 gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgc
     4861 ggccggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5221 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5521 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5881 atttccccga aaagtgccac ctgcataatg aaagacccca cctgtaggtt tggcaagcta
     5941 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agaaaagttc
     6001 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     6061 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     6121 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     6181 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     6241 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     6301 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     6361 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     6421 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     6481 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     6541 caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc
     6601 tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta
     6661 tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac cctgggagac
     6721 gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt
     6781 tttggactct ttggtgcacc ccccttagag gagggatatg tggttctggt aggagacgag
     6841 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga ccgaagccgc
     6901 gccgcgcgtc ttg
//
