LOCUS       sequence-252179-       19960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 
            translation system from E. coli .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19960)
  AUTHORS   Shepherd TR, Du L, Liljeruhm J, Samudyata, Wang J, Sjodin MOD, 
            Wetterhall M, Yomo T, Forster AC
  TITLE     De novo design and synthesis of a 30-cistron translation-factor 
            module.
  JOURNAL   Nucleic Acids Res. 2017 Oct 13;45(18):10895-10905. doi: 
            10.1093/nar/gkx753.
  PUBMED    28977654
REFERENCE   2  (bases 1 to 19960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-10-13- 13"; volume: "45"; issue: 
            "18"; pages: "10895-10905"
COMMENT     Sequence Label: pLD2 translation factors
FEATURES             Location/Qualifiers
     source          1..19960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1590..1637
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1674..1692
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1693..1717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1732..1754
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4576..4593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4679..4726
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4763..4781
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4782..4806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4821..4843
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4854..4871
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7582..7629
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        7666..7684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7685..7709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7724..7746
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7760..7777
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      11246..11293
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        11330..11348
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    11349..11373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             11388..11410
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             12363..12380
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      12466..12513
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        12550..12568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    12569..12593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             12608..12630
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             13487..13504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      13590..13637
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        13674..13692
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    13693..13717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             13732..13754
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             13765..13782
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             14272..14283
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      14392..14439
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        14476..14494
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    14495..14519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             14534..14556
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             14567..14584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      14870..14917
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    14932..14952
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     rep_origin      14971..15426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15058..15077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15268..15289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(15518..16333)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(15518..16333)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     16241..16260
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     16325..16344
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      16455..17043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16944..16963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17197..17214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    17229..17368
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(17294..17313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17454..17476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(17470..17661)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(18470..19552)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     19514..19533
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(19553..19630)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     19836..19855
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    19925..19946
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttccccacta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gcaccaccac caccaccaca gcgttgtgcc
      121 tgtagccgac gtactccagg gccgtgtagc cgttgacagc gaagtcaccg tgcgcggatg
      181 ggtacgtacc cgccgagatt caaaagctgg catctccttc ctcgccgttt atgacggttc
      241 ctgctttgat cctgtacagg ctgtcatcaa taattctctg cccaattaca atgaagacgt
      301 cctgcgtctg accaccggct gctcggtcat tgtgacgggt aaagtcgtgg cgtcgccggg
      361 ccaggggcaa caatttgaaa tccaggccag caaggttgaa gttgctggtt gggttgaaga
      421 tccagacact tacccgatgg cggcaaaacg ccacagcatt gagtatctgc gtgaagtcgc
      481 tcacctgcgt ccgcgcacaa acctgattgg tgccgtcgcg cgcgttcgcc atacgctggc
      541 gcaggcgctg catcgcttct ttaacgagca gggattcttc tgggtttcaa cgccactgat
      601 taccgcatct gataccgaag gtgcaggcga aatgttccgc gtttctacgc tggatctgga
      661 aaacctgccg cgtaacgatc agggcaaagt ggatttcgac aaagacttct ttggtaaaga
      721 gtctttcctg accgtatctg gccagttgaa cggcgaaacc tacgcttgcg cattgtccaa
      781 aatctatacc ttcggcccga ctttccgtgc tgaaaactcc aacaccagcc gtcacctggc
      841 ggagttctgg atgctggagc cggaagtggc gtttgctaac ctgaacgata ttgcgggtct
      901 ggctgaagcc atgctgaaat atgtcttcaa agcggttctc gaagaacgcg ctgacgacat
      961 gaagttcttc gctgaacgcg tagataaaga tgccgtttca cgtctggaac gcttcattga
     1021 agccgatttt gcgcaggtgg attataccga cgcagtgacc attctcgaaa actgcggcag
     1081 gaagtttgaa aacccggttt actggggagt cgatctctct tctgagcatg agcgttatct
     1141 ggcggaagaa cactttaaag caccggtagt ggttaaaaac tatccgaaag atattaaagc
     1201 gttctatatg cgccttaacg aagacggtaa aaccgttgcg gctatggacg ttctggctcc
     1261 gggcatcggt gagatcattg gtggctccca gcgtgaagaa cgtctggacg tgctggacga
     1321 gcgtatgctg gaaatgggcc tgaataaaga agattactgg tggtatcgcg atctgcgtcg
     1381 ctacggtact gttccgcatt caggtttcgg tcttggtttt gaacgtctga ttgcttacgt
     1441 aactggcgtg caaaacgtac gtgatgtgat tccgttccca cgtactccgc gtaacgccag
     1501 cttctaatta tctgttagtt tttttctact agactgctaa caaagcccga aaggaagctg
     1561 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     1621 tcttgagggg ttttttgctg aaaggaggaa ctactagagc gatcccgcga aattaatacg
     1681 actcactata ggggaattgt gagcggataa caattcccca ctagaaataa ttttgtttaa
     1741 ctttaagaag gagatataca tatgagtgac tataaatcaa ccctgaatct gccggaaaca
     1801 gggttcccga tgcgtggcga tctcgccaag cgcgaacccg gaatgctggc gcgttggact
     1861 gatgatgatc tgtacggcat catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg
     1921 catgatggcc ctccttatgc gaatggcagc attcatattg gtcactcggt taacaagatt
     1981 ctgaaagaca ttatcgtgaa gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct
     2041 ggctgggact gccacggtct gccgatcgag ctgaaagtcg agcaagaata cggtaagccg
     2101 ggtgagaagt tcaccgccgc cgagttccgc gccaagtgcc gcgaatacgc ggcgacccag
     2161 gttgacggtc aacgcaaaga ctttatccgt ctgggcgtgc tgggcgactg gtcgcacccg
     2221 tacctgacca tggacttcaa aactgaagcc aacatcatcc gcgcgctggg caaaatcatc
     2281 ggcaacggtc acctgcacaa aggcgcgaag ccagttcact ggtgcgttga ctgccgttct
     2341 gcgctggcgg aagcggaagt tgagtattac gacaaaactt ctccgtccat cgacgttgct
     2401 ttccaggcag tcgatcagga tgcactgaaa gcaaagtttg ccgtaagcaa cgttaacggc
     2461 ccaatctcgc tggtaatctg gaccaccacg ccgtggactc tgcctgccaa ccgcgcaatc
     2521 tctattgcac cagatttcga ctatgcgctg gtgcagatcg acggtcaggc cgtgattctg
     2581 gcgaaagatc tggttgaaag cgtaatgcag cgtatcggcg tgaccgatta caccattctc
     2641 ggcacggtaa aaggtgcgga gcttgagctg ctgcgcttta cccatccgtt tatgggcttc
     2701 gacgttccgg caatcctcgg cgatcacgtt accctggatg ccggtaccgg tgccgttcac
     2761 accgcgcctg gccacggccc ggacgactat gtgatcggtc agaaatacgg cctggaaacc
     2821 gctaacccgg ttggcccgga cggcacttat ctgccgggca cttatccgac gctggatggc
     2881 gtgaacgtct tcaaagcgaa cgacatcgtc gttgcgctgc tgcaagaaaa aggcgcgctg
     2941 ctgcacgttg agaaaatgca gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc
     3001 atcttccgcg cgacgccgca gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag
     3061 tcactgaaag agatcaaagg cgtgcagtgg atcccggact ggggccaggc gcgtatcgag
     3121 tcgatggttg ctaaccgtcc tgactggtgt atctcccgtc agcgcacctg gggtgtaccg
     3181 atgtcactgt tcgtgcacaa agacacggaa gagctgcatc cgcgtaccct tgaactgatg
     3241 gaagaagtgg caaaacgcgt tgaagtcgat ggcatccagg cgtggtggga tctcgatgcg
     3301 aaagagatcc tcggcgacga agctgatcag tacgtgaaag tgccggacac attggatgta
     3361 tggtttgact ccggatctac ccactcttct gttgttgacg tgcgtccgga gtttgccggt
     3421 cacgcagcgg acatgtatct ggaaggttct gaccaacacc gcggctggtt catgtcttcc
     3481 ctaatgatct ccaccgcgat gaagggtaaa gcgccgtatc gtcaggtact gacccacggc
     3541 tttaccgtgg atggtcaggg ccgcaagatg tctaaatcca tcggcaatac cgtttcgccg
     3601 caggatgtga tgaacaaact gggcgcggat attctgcgtc tgtgggtggc atcaaccgac
     3661 tacaccggtg aaatggccgt ttctgacgag atcctgaaac gtgctgccga tagctatcgt
     3721 cgtatccgta acaccgcgcg cttcctgctg gcaaacctga acggttttga tccagcaaaa
     3781 gatatggtga aaccggaaga gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa
     3841 gcggcacagg aagacatcct caaggcgtac gaagcatacg atttccacga agtggtacag
     3901 cgtctgatgc gcttctgctc cgttgagatg ggttccttct acctcgacat catcaaagac
     3961 cgtcagtaca ccgccaaagc ggacagtgtg gcgcgtcgta gctgccagac tgcgctatat
     4021 cacatcgcag aagcgctggt gcgctggatg gcaccaatcc tctccttcac cgctgatgaa
     4081 gtgtggggct acctgccggg cgaacgtgaa aaatacgtct tcaccggtga gtggtacgaa
     4141 ggcctgtttg gcctggcaga cagtgaagcg atgaacgatg cgttctggga cgagctgttg
     4201 aaagtgcgtg gcgaagtgaa caaagtcatt gagcaagcgc gtgccgacaa gaaagtgggt
     4261 ggctcgctgg aagcggcagt aaccttgtat gcagaaccgg aactgtcggc gaaactgacc
     4321 gcgctgggcg atgaattacg atttgtcctg ttgacctccg gcgctaccgt tgcagactat
     4381 aacgacgcac ctgctgatgc tcagcagagc gaagtactca aagggctgaa agtcgcgttg
     4441 agtaaagccg aaggtgagaa gtgcccacgc tgctggcact acacccagga tgtcggcaag
     4501 gtggcggaac acgcagaaat ctgtggccgc tgtgtcagca acgtcgccgg tgacggtgaa
     4561 aaacgtaagt ttgcccacca ccaccaccac cactaattat ctgttagttt ttttctacta
     4621 gactgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     4681 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     4741 tactagagcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
     4801 aattccccac tagaaataat tttgtttaac tttaagaagg agatatacat atgcaccacc
     4861 accaccacca cagcaagagc accgctgaga tccgtcaggc gtttctcgac tttttccata
     4921 gtaagggaca tcaggtagtt gccagcagct ccctggtacc ccataacgac ccaactttgt
     4981 tgtttaccaa cgccgggatg aaccagttca aggatgtgtt ccttgggctc gacaagcgta
     5041 attattcccg cgctaccact tcccaacgct gcgtgcgtgc gggtggtaaa cacaacgacc
     5101 tggaaaacgt cggttacacc gcgcgtcacc ataccttctt cgaaatgctg ggcaacttca
     5161 gcttcggcga ctatttcaaa cacgatgcca ttcagtttgc atgggaactg ctgaccagcg
     5221 aaaaatggtt tgccctgccg aaagagcgtc tgtgggttac cgtctatgaa agcgacgacg
     5281 aagcctacga aatctgggaa aaagaagtag ggatcccgcg cgaacgtatt attcgcatcg
     5341 gcgataacaa aggtgcgcca tacgcatctg acaacttctg gcagatgggt gacactggtc
     5401 cgtgcggccc gtgcaccgaa atcttctacg atcacggcga ccacatttgg gggggccctc
     5461 cgggaagccc ggaagaagac ggcgaccgct acattgagat ctggaacatc gtcttcatgc
     5521 agttcaaccg ccaggccgat ggcacgatgg aaccgctgcc gaagccgtct gtagataccg
     5581 gtatgggtct ggagcgtatt gctgcggtgc tgcaacacgt taactctaac tatgacatcg
     5641 acctgttccg cacgctgatc caggcggtag cgaaagtcac tggcgcaacc gatctgagca
     5701 ataaatcgct gcgcgtaatc gctgaccaca ttcgttcttg tgcgttcctg atcgcggatg
     5761 gcgtaatgcc gtccaatgaa aaccgtggtt atgtactgcg tcgtatcatt cgtcgcgcag
     5821 tgcgtcacgg taatatgctc ggcgcgaaag aaaccttctt ctacaaactg gttggtccgc
     5881 tgatcgacgt tatgggctct gcgggtgaag acctgaaacg ccagcaggcg caggttgagc
     5941 aggtgctgaa gactgaagaa gagcagtttg ctcgtactct ggagcgcggt ctggcgttgc
     6001 tggatgaaga gctggcaaaa ctttctggtg atacgctgga tggtgaaact gctttccgtc
     6061 tgtacgacac ctatggcttc ccggttgacc tgacggctga tgtttgtcgt gagcgcaaca
     6121 tcaaagttga cgaagctggt tttgaagctg caatggaaga gcagcgtcgt cgcgcgcgcg
     6181 aagccagcgg ctttggtgcc gattacaacg caatgatccg tgttgacagt gcatctgagt
     6241 ttaaaggcta tgaccatctg gaactgaacg gcaaagtgac tgcgctgttt gttgatggta
     6301 aagcggttga tgccatcaat gcaggccagg aagctgtggt cgtgctggat caaacgccat
     6361 tctatgcgga atccggcggt caggttggcg ataaaggcga actgaaaggc gctaacttct
     6421 cctttgcggt ggaagatacg cagaaatacg gccaggcgat tggtcacatc ggtaaacttg
     6481 ctgcgggttc tctgaaagtg ggcgacgcgg tgcaggctga tgttgatgag gctcgtcgcg
     6541 cccgtattcg tctgaatcac tccgcaacgc acctgatgca cgctgcgctg cgccaggttc
     6601 tgggtactca tgtatcgcag aaaggttcac tggttaacga caaggtgctg cgcttcgact
     6661 tctcacacaa cgaagcgatg aaaccagaag agattcgtgc ggtcgaagac ctggtgaaca
     6721 cacagattcg tcgcaatttg ccgatcgaaa ccaacatcat ggatctcgaa gcggcgaaag
     6781 cgaaaggtgc gatggcgctg ttcggcgaga agtatgatga gcgcgtacgc gtgctgagca
     6841 tgggcgattt ctctaccgag ttgtgtggcg gtactcacgc cagccgcact ggtgatattg
     6901 gtctgttccg catcatctct gaatcgggta ctgctgccgg cgttcgtcgt atcgaagcgg
     6961 taaccggaga aggtgctatc gccaccgttc atgcagacag cgatcgctta agcgaagtcg
     7021 cgcatctgct gaaaggcgat agcaataatc tggctgataa agtgcgctca gtactggaac
     7081 gtacgcgtca gctggaaaaa gagttacaac agcttaaaga acaagctgcc gcacaggaga
     7141 gcgcaaatct ttccagtaag gcaatcgatg ttaatggtgt taagctgttg gttagcgagc
     7201 ttagcggtgt tgagccgaaa atgttgcgta ccatggttga cgatttaaaa aatcagctgg
     7261 ggtcgacaat tatcgtgctg gcaacggtag tcgaaggtaa ggtttctctg attgcaggcg
     7321 tatctaagga cgtcacagat cgtgtgaaag caggggaact gattggtatg gtcgctcagc
     7381 aggtgggcgg caagggtggt ggacgtcctg acatggcgca agccggtggt acggatgctg
     7441 cggccttacc ggcagcgtta gccagtgtga aaggctgggt cagcgcgaaa ttgcaataat
     7501 tatctgttag tttttttcta ctagactgct aacaaagccc gaaaggaagc tgagttggct
     7561 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     7621 ggttttttgc tgaaaggagg aactactaga gcgatcccgc gaaattaata cgactcacta
     7681 taggggaatt gtgagcggat aacaattccc cactagaaat aattttgttt aactttaaga
     7741 aggagatata catatgatgc accaccacca ccaccactca catctcgcag aactggttgc
     7801 cagtgcgaag gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt
     7861 cgaatatttg ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc
     7921 gccagaagag cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca
     7981 ggcgctgaat gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc
     8041 ggaaacgatt gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt
     8101 tacccgtacc atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac
     8161 cgggccggaa atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca
     8221 cccggcgcgc gctgaccacg acactttctg gtttgacact acccgcctgc tgcgtaccca
     8281 gacctctggc gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc
     8341 gcctggccgt gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat
     8401 ggaaggtctg attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga
     8461 cttcctgcgt aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc
     8521 gtttaccgaa ccttctgcgg aagtggacgt catgggtaaa aacggtaaat ggctggaagt
     8581 gctgggctgc gggatggtgc atccgaacgt gttgcgtaac gttggcatcg acccggaagt
     8641 ttactctggt ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt
     8701 caccgacctg cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg
     8761 caggaataga ttatgaagtt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt
     8821 gatagcgatg cgctggcaaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa
     8881 ccggttgccg gcagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat
     8941 ccgaacgctg acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac
     9001 atcgtctgcg gtgcgccaaa ctgccgtcag ggcctgcgtg tagcggtagc gaccattggt
     9061 gctgttctgc cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa
     9121 gggatgctgt gctccttctc tgaactgggc atttctgacg atcacagcgg cattatcgaa
     9181 ctgcctgcgg atgcgccgat tggcaccgat atccgtgaat acctgaaact tgatgacaac
     9241 accatcgaaa tcagcgtgac gccaaaccgt gccgactgct taggcatcat tggtgttgcg
     9301 cgtgacgttg ccgtgctgaa ccagctgccg ctggttcaac cggaaatcgt tccggttggt
     9361 gcgaccatcg acgacacgct gccgattaca gtcgaagcgc cggaagcctg cccgcgttat
     9421 cttggccgtg tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa
     9481 aaactgcgtc gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgtg
     9541 ctgctcgaac tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt
     9601 gtggtgcgga tggcgaaaga gggcgaaacg ctggtgctgc tcgacggtac tgaagcgaag
     9661 ctgaatgctg acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc
     9721 ttcggtggcg aacactctgg cgtgaatgac gaaacacaaa acgtgctgct ggaatgcgcg
     9781 ttctttagcc cgctgtctat caccggtcgt gctcgtcgtc atggcctgca taccgatgcg
     9841 tctcaccgtt atgagcgtgg cgttgatccg gcactgcaac acaaagcgat ggaacgtgcg
     9901 acccgtctgc tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac
     9961 gaagcaacgc tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg
    10021 atcggccatc atattgcgga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa
    10081 gtgaccgaag gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag
    10141 attgaagaag atctggttga agaagtcgcg cgtgtttacg gctacaacaa catcccggat
    10201 gagccggtac aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag
    10261 cgcgtgaaaa cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt
    10321 gatccgaaag tgcagcagat gatccatcca ggcgttgaag ccttactgct gccaagcccg
    10381 atctctgttg aaatgtcagc aatgcgtctt tctctgtgga ctggcctgct ggcaaccgtg
    10441 gtgtacaacc agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc
    10501 gtaccagata ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt
    10561 tgcggtaacc gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat
    10621 ttgaaaggcg atcttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc
    10681 cgtgcagaag cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt
    10741 gaacgtattg gttttgttgg ggttgttcat cctgaactgg aacgtaaact ggatcttaac
    10801 ggtcgcactc tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag
    10861 gcgcgcgaga tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa
    10921 aacgttcccg cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt
    10981 ggcgtaaact tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc
    11041 gccataagcc tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct
    11101 accgtcgcca aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga
    11161 taattatctg ttagtttttt tctactagac tgctaacaaa gcccgaaagg aagctgagtt
    11221 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
    11281 gaggggtttt ttgctgaaag gaggaactac tagagcgatc ccgcgaaatt aatacgactc
    11341 actatagggg aattgtgagc ggataacaat tccccactag aaataatttt gtttaacttt
    11401 aagaaggaga tatacatatg tcagaatcac tacgtattat ttttgcgggt acacctgact
    11461 ttgcagcgcg tcatctcgac gcgctgttgt cttctggtca taacgtcgtt ggcgtgttca
    11521 cccagccaga ccgaccggca ggacgcggta aaaaactgat gcccagcccg gttaaagttc
    11581 tggctgagga aaaaggtctg cccgtttttc aacctgtttc cctgcgtcca caagaaaacc
    11641 agcaactggt cgccgaactg caagctgatg ttatggtcgt cgtcgcctat ggtttaattc
    11701 tgccgaaagc agtgctggag atgccgcgtc ttggctgtat caacgttcat ggttcactgc
    11761 tgccacgctg gcgcggtgct gcaccaatcc aacgctcact atgggcgggt gatgcagaaa
    11821 ctggtgtgac cattatgcaa atggatgtcg gtttagacac cggtgatatg ctctataagc
    11881 tctcctgccc gattaccgca gaagatacca gtggtacgct gtacgacaag ctggcagagc
    11941 ttggcccaca agggcttatc accacgttga aacaactggc agacggcacg gcgaaaccag
    12001 aagttcagga cgaaactctt gtcacttacg ccgagaagtt gagtaaagaa gaagcgcgta
    12061 ttgactggtc actttcggca gcacagcttg aacgctgcat tcgcgctttc aatccatggc
    12121 caatgagctg gctggaaatt gaaggacagc cggttaaagt ctggaaagca tcggtcattg
    12181 atacggcaac caacgctgca ccaggaacga tccttgaagc caacaaacaa ggcattcagg
    12241 ttgcgactgg tgatggcatc ctgaacctgc tctcgttaca acctgcgggt aagaaagcga
    12301 tgagcgcgca agacctcctg aactctcgtc gggaatggtt tgttccgggc aaccgtctgg
    12361 tccaccacca ccaccaccac taattatctg ttagtttttt tctactagac tgctaacaaa
    12421 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
    12481 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactac tagagcgatc
    12541 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tccccactag
    12601 aaataatttt gtttaacttt aagaaggaga tatacatatg gctgaaatta ccgcatccct
    12661 ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa aagcactgac
    12721 tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg gtgctattaa
    12781 agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca aaatcgacgg
    12841 caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa aagacgctgg
    12901 tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa tcactgacgt
    12961 tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga aaattggtga
    13021 aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt cttatcagca
    13081 cggtgcgcgt atcggcgttc tggttgctgc taaaggcgct gacgaagagc tggttaaaca
    13141 catcgctatg cacgttgctg caagcaagcc agagttcatc aaaccggaag acgtatccgc
    13201 tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt ctggtaagcc
    13261 gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aagttcaccg gcgaagtttc
    13321 tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc tgctgaaaga
    13381 gcataacgct gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca tcgagaaagt
    13441 tgagactgac tttgcagcag aagttgctgc gatgtccaag cagtctcacc accaccacca
    13501 ccactaatta tctgttagtt tttttctact agactgctaa caaagcccga aaggaagctg
    13561 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
    13621 tcttgagggg ttttttgctg aaaggaggaa ctactagagc gatcccgcga aattaatacg
    13681 actcactata ggggaattgt gagcggataa caattcccca ctagaaataa ttttgtttaa
    13741 ctttaagaag gagatataca tatgcaccac caccaccacc acaaaggcgg aaaacgagtt
    13801 caaacggcgc gccctaaccg tatcaatggc gaaattcgcg cccaggaagt tcgcttaaca
    13861 ggtctggaag gcgagcagct tggtattgtg agtctgagag aagctctgga gaaagcagaa
    13921 gaagccggag tagacttagt cgagatcagc cctaacgccg agccgccggt ttgtcgtata
    13981 atggattacg gcaaattcct ctatgaaaag agcaagtctt ctaaggaaca gaagaaaaag
    14041 caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg gtacagatga aggcgactat
    14101 caggtaaaac tccgcagcct gattcgcttt ctcgaagagg gtgataaagc caaaatcacg
    14161 ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg gtatggaagt gcttaatcgc
    14221 gtgaaagacg atttgcaaga actggcagtg gtcgaatcct tcccaacgaa gatcgaaggc
    14281 cgccagatga tcatggtgct cgctcctaag aagaaacagt aagaattgta ctagactgct
    14341 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
    14401 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactactaga
    14461 gcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
    14521 cactagaaat aattttgttt aactttaaga aggagatata catatgcacc accaccacca
    14581 ccacgcgaaa gaagataata ttgaaatgca aggcacggtc ctcgaaacgc ttccgaacac
    14641 gatgttccgg gtcgagctcg aaaacggcca cgtcgtgacg gcgcatatct cgggcaaaat
    14701 gcgcaaaaac tacatccgga tcctgaccgg cgacaaggtc accgtcgaac tcacgccgta
    14761 cgacctctcg aaaggccgca tcgtcttccg ctcgcgctaa gaattgtact agactgctaa
    14821 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
    14881 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctactagtag
    14941 cggccgctgc agtccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
    15001 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    15061 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    15121 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    15181 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    15241 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    15301 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    15361 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    15421 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    15481 aatacattca aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca
    15541 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
    15601 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
    15661 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
    15721 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
    15781 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
    15841 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
    15901 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
    15961 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
    16021 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
    16081 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
    16141 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
    16201 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
    16261 catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt
    16321 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
    16381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    16441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    16501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    16561 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    16621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    16681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    16741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    16801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    16861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    16921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    16981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    17041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    17101 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    17161 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    17221 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
    17281 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
    17341 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
    17401 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    17461 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
    17521 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
    17581 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
    17641 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
    17701 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
    17761 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
    17821 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
    17881 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
    17941 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
    18001 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
    18061 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
    18121 cacccgtggg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
    18181 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
    18241 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
    18301 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
    18361 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
    18421 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
    18481 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    18541 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
    18601 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
    18661 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
    18721 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
    18781 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
    18841 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
    18901 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
    18961 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
    19021 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
    19081 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
    19141 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
    19201 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
    19261 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
    19321 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
    19381 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
    19441 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
    19501 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
    19561 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
    19621 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
    19681 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
    19741 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
    19801 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
    19861 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag
    19921 atctgaattc gcggccgctt ctagagcgat cccgcgaaat
//
