LOCUS       sequence-25218-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Joberty G, Petersen C, Gao L, Macara IG
  TITLE     The cell-polarity protein Par6 links Par3 and atypical protein 
            kinase C to Cdc42.
  JOURNAL   Nat Cell Biol 2000 Aug;2(8):531-9.
  PUBMED    10934474
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol"; date: "2000-08"; volume: "2(8)"; pages: "531-9"
COMMENT     Sequence Label: pKMyc
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..87
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    147..281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(184..203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     238..257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        350..707
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        350..541
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(373..393)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      558..693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(727..744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(727..743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(736..758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggtgtccact cccaggtcca actgcacctc ggttctaagc ttctgcaggt cgacatggaa
       61 cagaaactca tctctgaaga ggatctgtct agaggatccc gggctagcgg gcggccgctt
      121 gaattcatcg atggccgcca tggcccaact tgtttattgc agcttataat ggttacaaat
      181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      241 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgggaattaa ttcggcgcag
      301 caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa
      361 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
      421 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      481 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      661 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctgttaacag
      721 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      781 taatc
//
