LOCUS       sequence-252181-        7053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse CD72a in animal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7053)
  TITLE     Tsubata CD72, SmB, and U1-C plasmids
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMXCD72a-ires-YFP
FEATURES             Location/Qualifiers
     source          1..7053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             152..568
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     498..520
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     536..552
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    578..952
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     890..906
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(2720..2722,2723..2725,2726..3439)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2765..2786)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3026..3045)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3373..3394
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3610..4079
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4209..4226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4380..4968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4460..4479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5132..5923,5924..5992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5755..5774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5993..6097)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6127..6720
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    join(6783..7053,1..87)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg
       61 aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg tgttgtctct gtctgactgt
      121 gtttctgtat ttgtctgaaa atatgggccc gggccagact gttaccactc ccttaagttt
      181 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
      241 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
      301 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
      361 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
      421 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
      481 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
      541 tccagccctc actccttctc taggcgcccc catatggcca tatgagatct tatatggggc
      601 acccccgccc cttgtaaact tccctgaccc tgacatgaca agagttacta acagcccctc
      661 tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga gacctctggc
      721 ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc gagtcggcga
      781 cacagtgtgg gtccgccgac accagactaa gaacctagaa cctcgctgga aaggacctta
      841 cacagtcctg ctgaccaccc ccaccgccct caaagtagac ggcatcgcag cttggataca
      901 cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac tgccggatcc
      961 cagtgtggtg gtacgggaat tcatggctga cgctatcacg tatgcagacc tgcgctttgt
     1021 gaaagtgccc ctgaagaaca gcgcatctaa ccatctagga caggactgtg aggcctatga
     1081 agatggggaa ctcacctacg agaacgtgca agtgtctcca gtcccaggag ggccaccagg
     1141 cttggcttcc cctgcactag cggacaaagc aggggtcggg tcagagcaac caactgcgac
     1201 ctggagctct gtgaagtcgt ctgctctcag gcagattccc cgctgtccta cggtctgctt
     1261 gcaaaacttc ttgcttggcc ttctcctgtc ctgtctgatg ttaggggtgg ctgtcatctg
     1321 cctgggagtt cgctatctgc aggtgtctca gcagttccag gaggggacca ggatttggga
     1381 agccaccaat agcagcctgc agcagcagct cagggagaag ataagtcagc tggggcagaa
     1441 ggaggtggag cttcaggagt ctcagaaaga gctgatctcg agccaggaca cattacagga
     1501 gaagcagagg actcacaagg acactgagca gcaactacaa gcctgccagg ctgagagagc
     1561 gaagaccaag gagaacctga aaactgagga ggagcggagg agggacctgg accagaggtt
     1621 gacaagcacg cgggagacac tgaggcgctt gtcctcctgt tcatcagaca cctgctgtcc
     1681 atgcggatgg attccatatc aggaaaggtg cttttacatc tcacataccc tcagaagtct
     1741 ggaggagagc caaaaatact gcacatctct gtcctccaaa ctggcagcat tcgatgaacc
     1801 ttctaagtat tactatgaag tttctctgcc cagcggctta gaggagttgc tagatcgttc
     1861 gaagtcatat tggatacaga tgagcaagaa gtggaggcat gactatgact ctcaaagccg
     1921 atattgtgac aagataaaaa aatattacca gaagtggaaa agaacatttt ctgagtgtgc
     1981 agagcttcac ccctgcattt gtgagtcgga ggctttcagg tttcctgatg ggatccatct
     2041 gaactgaacc ggatagcggc cgcgtcgagc agctgaagct taaaacagct ctggggttgt
     2101 acccacccca gaggcccacg tggcggctag tactccggta ttgcggtacc cttgtacgcc
     2161 tgttttatac tcccttcccg taacttagac gcacaaaacc aagttcaata gaagggggta
     2221 caaaccagta ccaccacgaa caagcacttc tgtttccccg gtgatgtcgt atagactgct
     2281 tgcgtggttg aaagcgacgg atccgttatc cgcttatgta cttcgagaag cccagtacca
     2341 cctcggaatc ttcgatgcgt tgcgctcagc actcaacccc agagtgtagc ttaggctgat
     2401 gagtctggac atccctcacc ggtgacggtg gtccaggctg cgttggcggc ctacctatgg
     2461 ctaacgccat gggacgctag ttgtgaacaa ggtgtgaaga gcctattgag ctacataaga
     2521 atcctccggc ccctgaatgc ggctaatccc aacctcggag caggtggtca caaaccagtg
     2581 attggcctgt cgtaacgcgc aagtccgtgg cggaaccgac tactttgggt gtccgtgttt
     2641 ccttttattt tattgtggct gcttatggtg acaatcacag attgttatca taaagcgaat
     2701 tggattgcgg ccggccgcaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2761 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2821 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2881 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
     2941 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3001 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3061 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3121 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3181 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3241 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3301 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     3361 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3421 ggacgagctg tacaagtaag tcgacgataa aataaaagat tttatttagt ctccagaaaa
     3481 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     3541 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     3601 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     3661 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3721 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     3781 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     3841 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     3901 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     3961 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     4021 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     4081 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     4141 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4801 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4981 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5041 atgagattat caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct
     5101 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5161 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5221 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5281 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5341 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5401 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5461 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5521 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5581 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5641 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5701 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5761 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5821 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5881 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5941 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6001 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6061 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6121 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     6181 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga
     6241 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     6301 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     6361 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     6421 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     6481 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     6541 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt
     6601 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     6661 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
     6721 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
     6781 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
     6841 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
     6901 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc
     6961 cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct ttggtgcacc
     7021 ccccttagag gagggatatg tggttctggt agg
//
