LOCUS       sequence-252187-        2800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses 
            amilCP_Orange chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: amilCP_Orange chromoprotein
FEATURES             Location/Qualifiers
     source          1..2800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        69..103
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    799..819
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      820..877
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(901..918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1072..1660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1152..1171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1843..1937)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1958..2617)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2532..2551
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2779..2797
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaaagaggag
      121 aaatactaga tgagtgtgat cgctaaacaa atgacctaca aggtttatat gtcaggcacg
      181 gtcaatggac actactttga ggtcgaaggc gatggaaaag gtaagcccta cgagggggag
      241 cagacggtaa agctcactgt caccaagggc ggacctctgc catttgcttg ggatatttta
      301 tcaccacagg ttggatacgg aagcatacca ttcaccaagt accctgaaga catccctgac
      361 tatgtaaagc agtcattccc ggagggctat acatgggaga ggatcatgaa ctttgaagat
      421 ggtgcagtgt gtactgtcag caatgattcc agcatccaag gcaactgttt catctaccat
      481 gtcaagttct ctggtttgaa ctttcctccc aatggacctg tcatgcagaa gaagacacag
      541 ggctgggaac ccaacactga gcgtctcttt gcacgagatg gaatgctgct aggaaacaac
      601 tttatggctc tgaagttaga aggaggcggt cactatttgt gtgaatttaa aactacttac
      661 aaggcaaaga agcctgtgaa gatgccaggg tatcactatg ttgaccgcaa actggatgta
      721 accaatcaca acaaggatta cacttcggtt gagcagtgtg aaatttccat tgcacgcaaa
      781 cctgtggtcg cctaataata ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
      841 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
      901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
     1081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1801 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     1861 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1921 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     1981 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2041 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2101 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2161 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     2221 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2281 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2341 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     2401 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     2461 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     2521 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     2581 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     2641 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     2701 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
     2761 atgacattaa cctataaaaa taggcgtatc acgaggcaga
//
