LOCUS       sequence-252193-        2818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: eforRed chromoprotein
FEATURES             Location/Qualifiers
     source          1..2818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        69..103
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    817..837
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      838..895
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(919..936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1090..1678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1170..1189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1861..1955)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1976..2635)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2550..2569
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2797..2815
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaaagaggag
      121 aaatactaga tgtcagtgat taagcaggta atgaagacca agttgcacct tgagggcact
      181 gtcaatggcc atgattttac gatcgagggt aaaggtgaag gcaagccgta cgaagggtta
      241 cagcacatga aaatgacagt caccaaaggc gcgcctctgc cgttttccgt tcatattctt
      301 acacctagcc acatgtatgg aagcaaaccg tttaataagt atccagcgga tatcccagac
      361 taccacaaac agtcttttcc cgaaggtatg tcttgggagc ggtcgatgat ttttgaagat
      421 ggtggcgtat gcaccgccag taatcactcc agcataaact tgcaagagaa ctgtttcatc
      481 tatgatgtta aatttcatgg tgtgaacctg cctccggatg ggcccgtaat gcaaaaaacc
      541 attgctggat gggagccgag cgtggaaaca ctgtacgtgc gtgacgggat gttaaaaagt
      601 gacactgcaa tggtttttaa actgaaagga ggcggtcatc atcgtgttga tttcaaaacg
      661 acgtataaag ccaaaaaacc tgtcaagctg ccagaatttc atttcgttga acatcgcctg
      721 gaactgacca aacacgataa agatttcaca acttgggacc agcaggaggc agccgaaggc
      781 catttctcac cgctgccgaa ggctctccca taataatact agtagcggcc gctgcagtcc
      841 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      901 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1081 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     1861 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1921 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1981 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     2041 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     2101 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2161 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     2221 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2281 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     2341 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2401 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2461 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2521 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2581 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2641 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2701 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     2761 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcaga
//
