LOCUS       sequence-252211-       12616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IGF1R F1057L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12616)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-IGF1R-F1057L
FEATURES             Location/Qualifiers
     source          1..12616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5331..5355)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5408..5449
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5470..6037
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5487..6039
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5631..5648)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5858..5877
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6051..6053,6054..6056,6057..6770)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6051..6053,6054..6056,6057..6767)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6096..6117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6357..6376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6704..6725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6784..7372
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6837..6857)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7255..7266)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7447..7680
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7799..7817)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7885..7989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7990..8058,8059..8850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8208..8227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9021..9609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9510..9529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9763..9780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10020..10041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10056..10061,10062..10079,10080..10086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10094..10110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10099..10121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10118..10134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10118..10134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10184..10203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10395..11028
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11075..11200
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11697..11930
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12115..12159
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12452..12569
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgaa gtctggctcc ggaggagggt ccccgacctc
     1261 gctgtggggg ctcctgtttc tctccgccgc gctctcgctc tggccgacga gtggagaaat
     1321 ctgcgggcca ggcatcgaca tccgcaacga ctatcagcag ctgaagcgcc tggagaactg
     1381 cacggtgatc gagggctacc tccacatcct gctcatctcc aaggccgagg actaccgcag
     1441 ctaccgcttc cccaagctca cggtcattac cgagtacttg ctgctgttcc gagtggctgg
     1501 cctcgagagc ctcggagacc tcttccccaa cctcacggtc atccgcggct ggaaactctt
     1561 ctacaactac gccctggtca tcttcgagat gaccaatctc aaggatattg ggctttacaa
     1621 cctgaggaac attactcggg gggccatcag gattgagaaa aatgctgacc tctgttacct
     1681 ctccactgtg gactggtccc tgatcctgga tgcggtgtcc aataactaca ttgtggggaa
     1741 taagccccca aaggaatgtg gggacctgtg tccagggacc atggaggaga agccgatgtg
     1801 tgagaagacc accatcaaca atgagtacaa ctaccgctgc tggaccacaa accgctgcca
     1861 gaaaatgtgc ccaagcacgt gtgggaagcg ggcgtgcacc gagaacaatg agtgctgcca
     1921 ccccgagtgc ctgggcagct gcagcgcgcc tgacaacgac acggcctgtg tagcttgccg
     1981 ccactactac tatgccggtg tctgtgtgcc tgcctgcccg cccaacacct acaggtttga
     2041 gggctggcgc tgtgtggacc gtgacttctg cgccaacatc ctcagcgccg agagcagcga
     2101 ctccgagggg tttgtgatcc acgacggcga gtgcatgcag gagtgcccct cgggcttcat
     2161 ccgcaacggc agccagagca tgtactgcat cccttgtgaa ggtccttgcc cgaaggtctg
     2221 tgaggaagaa aagaaaacaa agaccattga ttctgttact tctgctcaga tgctccaagg
     2281 atgcaccatc ttcaagggca atttgctcat taacatccga cgggggaata acattgcttc
     2341 agagctggag aacttcatgg ggctcatcga ggtggtgacg ggctacgtga agatccgcca
     2401 ttctcatgcc ttggtctcct tgtccttcct aaaaaacctt cgcctcatcc taggagagga
     2461 gcagctagaa gggaattact ccttctacgt cctcgacaac cagaacttgc agcaactgtg
     2521 ggactgggac caccgcaacc tgaccatcaa agcagggaaa atgtactttg ctttcaatcc
     2581 caaattatgt gtttccgaaa tttaccgcat ggaggaagtg acggggacta aagggcgcca
     2641 aagcaaaggg gacataaaca ccaggaacaa cggggagaga gcctcctgtg aaagtgacgt
     2701 cctgcatttc acctccacca ccacgtcgaa gaatcgcatc atcataacct ggcaccggta
     2761 ccggccccct gactacaggg atctcatcag cttcaccgtt tactacaagg aagcaccctt
     2821 taagaatgtc acagagtatg atgggcagga tgcctgcggc tccaacagct ggaacatggt
     2881 ggacgtggac ctcccgccca acaaggacgt ggagcccggc atcttactac atgggctgaa
     2941 gccctggact cagtacgccg tttacgtcaa ggctgtgacc ctcaccatgg tggagaacga
     3001 ccatatccgt ggggccaaga gtgagatctt gtacattcgc accaatgctt cagttccttc
     3061 cattcccttg gacgttcttt cagcatcgaa ctcctcttct cagttaatcg tgaagtggaa
     3121 ccctccctct ctgcccaacg gcaacctgag ttactacatt gtgcgctggc agcggcagcc
     3181 tcaggacggc tacctttacc ggcacaatta ctgctccaaa gacaaaatcc ccatcaggaa
     3241 gtatgccgac ggcaccatcg acattgagga ggtcacagag aaccccaaga ctgaggtgtg
     3301 tggtggggag aaagggcctt gctgcgcctg ccccaaaact gaagccgaga agcaggccga
     3361 gaaggaggag gctgaatacc gcaaagtctt tgagaatttc ctgcacaact ccatcttcgt
     3421 gcccagacct gaaaggaagc ggagagatgt catgcaagtg gccaacacca ccatgtccag
     3481 ccgaagcagg aacaccacgg ccgcagacac ctacaacatc accgacccgg aagagctgga
     3541 gacagagtac cctttctttg agagcagagt ggataacaag gagagaactg tcatttctaa
     3601 ccttcggcct ttcacattgt accgcatcga tatccacagc tgcaaccacg aggctgagaa
     3661 gctgggctgc agcgcctcca acttcgtctt tgcaaggact atgcccgcag aaggagcaga
     3721 tgacattcct gggccagtga cctgggagcc aaggcctgaa aactccatct ttttaaagtg
     3781 gccggaacct gagaatccca atggattgat tctaatgtat gaaataaaat acggatcaca
     3841 agttgaggat cagcgagaat gtgtgtccag acaggaatac aggaagtatg gaggggccaa
     3901 gctaaaccgg ctaaacccgg ggaactacac agcccggatt caggccacat ctctctctgg
     3961 gaatgggtcg tggacagatc ctgtgttctt ctatgtccag gccaaaacag gatatgaaaa
     4021 cttcatccat ctgatcatcg ctctgcccgt cgctgtcctg ttgatcgtgg gagggttggt
     4081 gattatgctg tacgtcttcc atagaaagag aaataacagc aggctgggga atggagtgct
     4141 gtatgcctct gtgaacccgg agtacttcag cgctgctgat gtgtacgttc ctgatgagtg
     4201 ggaggtggct cgggagaaga tcaccatgag ccgggaactt gggcaggggt cgtttgggat
     4261 ggtctatgaa ggagttgcca agggtgtggt gaaagatgaa cctgaaacca gagtggccat
     4321 taaaacagtg aacgaggccg caagcatgcg tgagaggatt gagtttctca acgaagcttc
     4381 tgtgatgaag gagctcaatt gtcaccatgt ggtgcgattg ctgggtgtgg tgtcccaagg
     4441 ccagccaaca ctggtcatca tggaactgat gacacggggc gatctcaaaa gttatctccg
     4501 gtctctgagg ccagaaatgg agaataatcc agtcctagca cctccaagcc tgagcaagat
     4561 gattcagatg gccggagaga ttgcagacgg catggcatac ctcaacgcca ataagttcgt
     4621 ccacagagac cttgctgccc ggaattgcat ggtagccgaa gatttcacag tcaaaatcgg
     4681 agattttggt atgacgcgag atatctatga gacagactat taccggaaag gagggaaagg
     4741 gctgctgccc gtgcgctgga tgtctcctga gtccctcaag gatggagtct tcaccactta
     4801 ctcggacgtc tggtccttcg gggtcgtcct ctgggagatc gccacactgg ccgagcagcc
     4861 ctaccagggc ttgtccaacg agcaagtcct tcgcttcgtc atggagggcg gccttctgga
     4921 caagccagac aactgtcctg acatgctgtt tgaactgatg cgcatgtgct ggcagtataa
     4981 ccccaagatg aggccttcct tcctggagat catcagcagc atcaaagagg agatggagcc
     5041 tggcttccgg gaggtctcct tctactacag cgaggagaac aagctgcccg agccggagga
     5101 gctggacctg gagccagaga acatggagag cgtccccctg gacccctcgg cctcctcgtc
     5161 ctccctgcca ctgcccgaca gacactcagg acacaaggcc gagaacggcc ccggccctgg
     5221 ggtgctggtc ctccgcgcca gcttcgacga gagacagcct tacgcccaca tgaacggggg
     5281 ccgcaagaac gagcgggcct tgccgctgcc ccagtcttcg acctgctaga acccagcttt
     5341 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     5401 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta
     5461 gtaatgagat ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     5521 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5581 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5641 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5701 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5761 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5821 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5881 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5941 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     6001 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca
     6061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     6121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     6181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     6241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     6301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     6361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     6421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     6481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     6541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     6601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat
     6781 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6961 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7021 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7081 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7141 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7201 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7261 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7321 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
     7381 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7441 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7501 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7561 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7621 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7681 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7741 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
     7801 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7861 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7921 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7981 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8041 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8101 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8161 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8221 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8281 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8341 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8401 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8461 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8521 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8581 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8641 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8701 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8761 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8821 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8881 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8941 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9001 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9061 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9841 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9901 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9961 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10021 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    10081 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
    10141 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10201 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10261 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10321 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10381 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
    10441 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
    10501 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
    10561 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
    10621 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
    10681 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
    10741 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
    10801 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
    10861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    11041 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
    11101 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    11161 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    11221 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    11281 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    11341 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    11401 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    11461 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    11521 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
    11581 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11641 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11701 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    11761 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    11821 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    11881 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    11941 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    12001 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    12061 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    12121 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    12181 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    12241 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    12301 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    12361 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
    12421 cgaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    12481 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    12541 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    12601 tttggactag tcgtga
//
