LOCUS       sequence-252214-        4512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for InaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PinaA-cfp
FEATURES             Location/Qualifiers
     source          1..4512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(680..774)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(805..1599)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(916..935)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1526..1545
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             2051..2062
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2835..2906
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2922..2949
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3480..4430)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 ggtgatctgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
       61 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
      121 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
      181 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
      241 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
      301 agcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
      361 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
      421 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
      481 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
      541 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
      601 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
      661 tactgtccct agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      721 tgaacaaatc cagatggagt tctgaggtca ttactggata tatcaacagg agtccaagcg
      781 agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
      841 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      901 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      961 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     1021 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     1081 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     1141 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     1201 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     1261 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     1321 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     1381 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     1441 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     1501 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     1561 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     1621 ctcttgatca gatcatgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     1681 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt
     1741 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgccaatgct tttcagcgtt
     1801 aactctggtt gtaaggcgat gcctaatccc tgtcgaataa aattcatcgc tgtatccgcc
     1861 tgattaaatt cacattttat ctgctgggcg cactattagt ggtagtggct ggtggaatct
     1921 atgcgctgca aatgaagggt aaaagcggtg tcaagctagt agtggcaaaa gaaattcatt
     1981 aatacgacac gtttcattaa gattttcctc aggtaaccac ctatagtcat tgtcgtggat
     2041 ccttaacttt aaagaggaga aaggtaccgc atgactagca aaagaagcaa aggtgaagaa
     2101 ctgttcactg gtgttgttcc aattctggtt gaactggatg gtgatgttaa tggtcacaaa
     2161 ttttctgtct ctggtgaggg tgaaggtgat gcaacctacg gtaaactgac cctgaaattt
     2221 atttgcacta ctggtaaact gcctgttccg tggccaaccc tggtcactac tctgacttgg
     2281 ggtgttcaat gctttgctcg ttacccagat cacatgaaac agcatgactt tttcaagtct
     2341 gccatgccgg aaggttatgt tcaggaacgt actatctttt tcaaagatga cggtaactac
     2401 aagacccgtg ctgaagtcaa gtttgaaggt gataccctgg ttaatcgtat cgagctgaaa
     2461 ggtattgatt ttaaagaaga tggtaacatt ctgggtcaca aactggaata caacgctatt
     2521 tctgataatg tatacatcac tgctgacaaa caaaagaatg gtatcaaagc taatttcaaa
     2581 attcgtcaca acattgaaga tggtagcgtt caactggcag accattatca acaaaatact
     2641 ccaattggcg atggccctgt cctgctgcca gacaaccatt acctgtccac ccaatctcgt
     2701 ctgtctaaag atccgaacga aaagcgcgat cacatggtcc tgctggagtt tgtaaccgct
     2761 gctggtatta ccctgggcat ggatgaactg tataaataat agggatccaa actcgagtaa
     2821 ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2881 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2941 ctttctgcgt ttatacctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt
     3001 tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc tgaaagcgct
     3061 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     3121 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     3181 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     3241 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     3301 ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     3361 caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg tgaacagttg
     3421 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     3481 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     3541 catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa ttttgcctca
     3601 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     3661 tgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     3721 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     3781 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     3841 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     3901 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     3961 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     4021 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     4081 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     4141 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     4201 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     4261 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     4321 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     4381 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     4441 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     4501 gtccttttcc cg
//
