LOCUS       sequence-252216-        4425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for PurA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PpurA-cfp
FEATURES             Location/Qualifiers
     source          1..4425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             681..692
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1465..1536
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1552..1579
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2110..3060)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3822..3916)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3947..4425,1..316))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4058..4077)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
       61 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
      121 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
      181 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
      241 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
      301 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat catgatcccc
      361 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      421 ccttaccaga gggcgcccca gctggcaatt ccgacgtcgg tgcctaatga gtgagctaac
      481 ttacattaat tgcgttgcgc ggcggtggac ttgtggttgc gggcgttgtg gtctactaca
      541 tgttgaggaa aacgattggc tgaacaaaaa acagactgat cgaggtcatt tttgagtgca
      601 aaaagtgctg taactctgaa aaagcgatgg tagaatccat ttttaagcaa acggtgattt
      661 tgaaaaggat ccttaacttt aaagaggaga aaggtaccgc atgactagca aaagaagcaa
      721 aggtgaagaa ctgttcactg gtgttgttcc aattctggtt gaactggatg gtgatgttaa
      781 tggtcacaaa ttttctgtct ctggtgaggg tgaaggtgat gcaacctacg gtaaactgac
      841 cctgaaattt atttgcacta ctggtaaact gcctgttccg tggccaaccc tggtcactac
      901 tctgacttgg ggtgttcaat gctttgctcg ttacccagat cacatgaaac agcatgactt
      961 tttcaagtct gccatgccgg aaggttatgt tcaggaacgt actatctttt tcaaagatga
     1021 cggtaactac aagacccgtg ctgaagtcaa gtttgaaggt gataccctgg ttaatcgtat
     1081 cgagctgaaa ggtattgatt ttaaagaaga tggtaacatt ctgggtcaca aactggaata
     1141 caacgctatt tctgataatg tatacatcac tgctgacaaa caaaagaatg gtatcaaagc
     1201 taatttcaaa attcgtcaca acattgaaga tggtagcgtt caactggcag accattatca
     1261 acaaaatact ccaattggcg atggccctgt cctgctgcca gacaaccatt acctgtccac
     1321 ccaatctcgt ctgtctaaag atccgaacga aaagcgcgat cacatggtcc tgctggagtt
     1381 tgtaaccgct gctggtatta ccctgggcat ggatgaactg tataaataat agggatccaa
     1441 actcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1501 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1561 tcgggtgggc ctttctgcgt ttatacctag ggtacgggtt ttgctgcccg caaacgggct
     1621 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc
     1681 tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
     1741 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
     1801 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
     1861 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
     1921 atctgttcat ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct
     1981 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg
     2041 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     2101 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     2161 ttgcgtgagc catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa
     2221 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     2281 tagtccgtta tgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     2341 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     2401 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     2461 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     2521 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     2581 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     2641 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     2701 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     2761 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     2821 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     2881 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     2941 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     3001 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     3061 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     3121 ataattacta gtccttttcc cgggtgatct gggtatctgt aaattctgct agacctttgc
     3181 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     3241 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     3301 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     3361 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     3421 aagtagcacc ctcgcaagct cgagcaaatc gctgaatatt ccttttgtct ccgaccatca
     3481 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     3541 tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca
     3601 taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg
     3661 gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac
     3721 ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta
     3781 tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac
     3841 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     3901 tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt
     3961 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     4021 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     4081 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     4141 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     4201 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     4261 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     4321 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     4381 gcattgcatc agccatgatg gatactttct cggcaggagc aaggt
//
