LOCUS       sequence-252218-        5635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  modular integrative vector for the assembly of DNA fragments and 
            expression in Streptomyces species.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Aubry C, Pernodet JL, Lautru S
  TITLE     A set of modular and integrative vectors for synthetic biology in 
            Streptomyces.
  JOURNAL   Appl Environ Microbiol. 2019 Jun 7. pii: AEM.00485-19. doi: 
            10.1128/AEM.00485-19.
  PUBMED    31175189
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2019 Jun 7. pii: AEM.00485-19. doi: 
            10.1128/AEM.00485-19."
COMMENT     Sequence Label: pOSV809
FEATURES             Location/Qualifiers
     source          1..5635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      845..916
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      932..959
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    3025..3058
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            3259..3368
                     /note="incP origin of transfer"
     CDS             complement(3766..4569)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3877..3896)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4487..4506
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(4822..4839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4956..5501)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5036..5055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5602..5635
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttatatacta gtagcggccg cttaagcgct ccctgcccgc tgtggtgacg aaggaactac
     1021 tcgttagcct aactaacgat ttcaaggcca aaaaaggggc cgaaccgtcc gccggctcga
     1081 cccctcccgc tgtgcgctac agcgccgcaa gctcccgctc agtggcttcg ctcgcttcgt
     1141 cttcctcctt cagcagctcc gcccacttga gcgtcacacg gtccttcagg gggatgtatt
     1201 tcttcgtctc agggtggcgc ccccggtcga ccatgacgga gtcaaggaag aagctcagga
     1261 actcccgacg ctcgtacacg tcccagcttg cccagatgcc cccttcggcc gtcgggtctt
     1321 cgccgctgaa ccactcagac ggaacgcggg tgctgccgtt catcttctcg tcaagctcag
     1381 cgagtcgggt cgtgcactcc gcttcgtagg accggtattg cagcaccgtt gagcgccacg
     1441 tttccagctc ttcacgcccg acgtacagac cggccttacg gtccgcctga aggtccttga
     1501 tggagcgccg cacgttgtct aggtgcgcct gttgttcgcg ccgctcatcg gccacccccg
     1561 ctaggtcgtg ctgaagggcg aagcgctccg cagcggcggc aatccatgcc tgatcgtgtt
     1621 catcttccat gtcggctgtg cggagccgtg cccacacctt cgaagcaacg aattcgtcca
     1681 gttcgctgcg cttgaccgac aagccgccgt gccccttcgg gttggcgcac atgtaattgc
     1741 cttcggcaag gtcgccgttg cgcttacggc caccctggga ctgacccatt gagccgccgc
     1801 agattcggca ccccaggaaa cgccacccgc tcagaagcgt cggttcgact tcggccccag
     1861 gcttccggtc gctgagattc ttcccggaac gcttctcttg aagctcaagc cacttcgagc
     1921 cgctgagaat gcccgtgtgg ggcgttagcg gcttgccgcc ggggtcgcgc cgtatgacgt
     1981 tgatgtgcgc cttaccgtgc ttcacacgct cgaatgcgaa accgccgatt gcgggatggt
     2041 tgagaatcca tcggaccgtt tgagcgcgcc acatgatcgg cttttcagcg ccgttcaggc
     2101 gacgtgcctt gatggacgca agacgctttt ccgtggcgcg tctctcagcc attccgggcg
     2161 acgggatctt ttccttctcg aaggtcgttg caatggcgtt gtcggacacg ccttcgaacg
     2221 acattttcgc catgcgctca actagctcga cgtgatccgg gttgtcttcg tccggctcaa
     2281 gaacggagat cacgagatta tcgaccttct tgcgcacggc gcgcattccg aacggggcgg
     2341 aagacgagtg aacgccaccc agcgcggcaa tctcgtcttt cgcacccttc aggcgctccg
     2401 ccttcaggtc actgtcctgt ttcgcaaggg cagcgatcag cgcgaaaatg gcgacgccga
     2461 taggggtaga cgtgtcaagg aacggctcaa gaaccgacat gaagcgcacg ccgtgcttct
     2521 tcaattcgtt gtcgatttcg agcgcgtcat gggcgccctt gcgagtgagc cgggaaagct
     2581 cattcacaac gacaacgtca ccttcgccgg cgcggacttc gcccatcatg cgctcgaagt
     2641 cggcacgggt cacgttcggg tcccagccgg agcgcccgac gtccacgaac tcacctgcca
     2701 cgacccagcg cgcacccccc tgggcgttgc gagacgcgac caacgcacga ccggccgcca
     2761 gctgtgcttc ggtcgacacg tctgagccgt cggaccggcc cttagactga cgcgcgtaca
     2821 ggaagacgcg aacagtgtct agaaggtgct cagggacgtc gggagcgatg aagggcgaca
     2881 tgcctagatc ttggctcatg ggcgctgacc tgcgctttct tttgggaaca ttggtggtgc
     2941 tgagtagttt cccatggatc actgtccaga gacaacaacc cagcacccga aacgtctgaa
     3001 gccccgtccg gcgccacctg cagggaagtt cctattctct agaaagtata ggaacttcgt
     3061 ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca
     3121 tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct
     3181 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
     3241 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     3301 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     3361 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     3421 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
     3481 agcagggtta tgcagcggaa aagggtacca cgtcgcggtg agttcaggct ttttcatatc
     3541 tcattgcccc cggacgagcg tctgctccgc cattcgccgt ccgccgtgcc aatcggatca
     3601 gccgtccaaa tgcgggattt tcgttagtcg gaggccaaac ggcattgagc gtcagcatat
     3661 catcagcgag ctgaagaaag acaatccccg atccgctcca cgtgttgccc cagcaatcag
     3721 cgcgaccttg cccctccaac gtcatctcgt tctccgctca tgagctcaga agaactcgtc
     3781 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     3841 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     3901 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     3961 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     4021 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     4081 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     4141 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     4201 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     4261 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     4321 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
     4381 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     4441 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     4501 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     4561 gcgagacatt gcactccacc gctgatgaca tcagtcgatc atagcacgat caacggcact
     4621 gttgcaaata gtcggtggtg ataaacttat catccccttt tgctgatgga gctgcacatg
     4681 aaccggatcc cctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     4741 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     4801 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     4861 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     4921 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     4981 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     5041 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     5101 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     5161 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     5221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     5281 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     5341 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     5401 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     5461 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     5521 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
     5581 gccccatacg atataagttg tgaagttcct attctctaga aagtatagga acttc
//
