LOCUS       sequence-252221-        5703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  modular integrative vector for the assembly of DNA fragments and 
            expression in Streptomyces species.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Aubry C, Pernodet JL, Lautru S
  TITLE     A set of modular and integrative vectors for synthetic biology in 
            Streptomyces.
  JOURNAL   Appl Environ Microbiol. 2019 Jun 7. pii: AEM.00485-19. doi: 
            10.1128/AEM.00485-19.
  PUBMED    31175189
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2019 Jun 7. pii: AEM.00485-19. doi: 
            10.1128/AEM.00485-19."
COMMENT     Sequence Label: pOSV810
FEATURES             Location/Qualifiers
     source          1..5703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      845..916
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      932..959
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1007..2824)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    2834..2933
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             3004..3015
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3093..3126
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            3327..3436
                     /note="incP origin of transfer"
     CDS             complement(3834..4637)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3945..3964)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4555..4574
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(4890..4907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5024..5569)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5104..5123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5670..5703
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttatatacta gtagcggccg cttaagcagg cttcccgggt gtctcgctac gccgctacgt
     1021 cttccgtgcc gtcctgggcg tcgtcttcgt cgtcgtcggt cggcggcttc gcccacgtga
     1081 tcgaagcgcg cttctcgatg ggcgttccct gccccctgcc cgtagtcgac ttcgtgacaa
     1141 cgatcttgtc tacgaagagc ccgacgaaca cgcgcttgtc gtctactgac gcgcgccccc
     1201 accacgactt agggccggtc gggtcagcgt cggcgtcttc ggggaaccat tggtcaaggg
     1261 gaagcttcgg ggcttcggcg gcttcaagtt cggcaagccg ctcttccgcc ccttgctgcc
     1321 ggagcgtcag cgctgcctgt tgcttccgga agtgcttcct gccaacgggt ccgtcgtacg
     1381 cgcctgccgc gcggtcttcg tacagctctt caagggcgtt cagggcgtcg gcgcgctccg
     1441 caacaaggtt cgcccgttcg ccgctcttct caggcgcctc agtgagcttg ccgaagcgtc
     1501 gggcggcttc ccacagaagc gccaacgtct cttcgtcgcc ttcggcgtgc ctgatcttgt
     1561 tgaagatgcg ttccgcaacg aacttgtcga gtgccgccat gctgacgttg cacgtgcctt
     1621 cgtgctgccc aggtgcggac gggtcgacca ccttccggcg acggcagcgg taagagtcct
     1681 tgatcgattc ttccccgcgc ttcgaagtca tgacggcgcc acactcgcag tacagcttgt
     1741 ccatggcgga cagaatggct tgcccccggg aaagcccctt gccgcgcccc ctgccgtcca
     1801 accacgcctg aagctcatac cactcagcgg gctcgatgat cggtccgcaa tcaagctcga
     1861 ccggccggag cgtgatcggg tcgcgctgaa tgcggtaacc ctcaatcttc gtggtcggcg
     1921 tgccgtccgg cttcttcttg tagatcacct cagcggcgaa gcccgcaata cgcgggtccc
     1981 gaaggattcg cataacggtt gccgggtccc aggcgcttga agcggtcttc ttcccaatcg
     2041 tctcgccccg ggtcggcacg gcgtcagcgt ccatgcgctt acaaagcccc gtgatgctgc
     2101 ccgggtgaat ggcggcttga ctgcccggct tgaagggaag gtgtttgtgc gtcttgatct
     2161 cacgccacca ccaccggatt acgtcgggct cgaactcgaa gggtccggta aggggagtgg
     2221 tcgagtgcgc aagcttgttg atgacgacat tgaccattcg gccgttgcgc gtgatctcct
     2281 tcgtctccga aacaagctcg aagccgtaag gcgccttccc gccgacgtac ccgcccaatt
     2341 cgcgctgaag gttcttcgtg tcgagaatct tcgccgactt cagcgaagat tctttgtgcg
     2401 acgcgtcgag ccgcataatc aggtgaatca ggtccatgac gtttccctgc cggaagacgc
     2461 cttcctgagt ggaaacaatc gtcacgccca gggcgagcaa ttccgagaca atcggaatcg
     2521 cgtccatgac cttcaggcgc gagaagcgcg acacgtcata gacaatgatc atgttgagcc
     2581 gcccggcgcg gcattcgttc aggatgcgtt cgaactccgg gcgctccgcc gtcccgaacg
     2641 ccgacgtgcc cggcgcttcg ctgaaatgcc cgacgaacct gaaccggccc ccgtcgcgct
     2701 cgacttcgcg ctgaaggtcg gccgccttgt cttcgttggc gctacgctgt gtcgctgggc
     2761 ttgctgcgct cgaattttcg cgctcgcgcg actgacggtc gtaagcaccc gcgtacgtgt
     2821 ccaccccggt cacaacccct tgtgtcatgt cggcgaccct acgcccccaa ctgagagaac
     2881 tcaaaggtta ccccagttgg ggcactactc ccgaaaaccg cttctgacct gggaaaacgt
     2941 gaagccccgg ggcatccgct gagggttgcc gccggggctt cggtgtgtcc gtcagtacgg
     3001 gccatagagg ggcgtcgtgt aacccaccca gggcgattgc tccggcacgg ggaagccgcg
     3061 ccacgccttc gggacgtctg gaatcctgca gggaagttcc tattctctag aaagtatagg
     3121 aacttcgtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga
     3181 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca
     3241 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt
     3301 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag
     3361 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac
     3421 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct
     3481 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat
     3541 gaccccgaag cagggttatg cagcggaaaa gggtaccacg tcgcggtgag ttcaggcttt
     3601 ttcatatctc attgcccccg gacgagcgtc tgctccgcca ttcgccgtcc gccgtgccaa
     3661 tcggatcagc cgtccaaatg cgggattttc gttagtcgga ggccaaacgg cattgagcgt
     3721 cagcatatca tcagcgagct gaagaaagac aatccccgat ccgctccacg tgttgcccca
     3781 gcaatcagcg cgaccttgcc cctccaacgt catctcgttc tccgctcatg agctcagaag
     3841 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     3901 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     3961 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     4021 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     4081 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     4141 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     4201 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     4261 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     4321 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     4381 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     4441 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     4501 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     4561 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     4621 tcaatcatgc gagacattgc actccaccgc tgatgacatc agtcgatcat agcacgatca
     4681 acggcactgt tgcaaatagt cggtggtgat aaacttatca tccccttttg ctgatggagc
     4741 tgcacatgaa ccggatcccc tagcggagtg tatactggct tactatgttg gcactgatga
     4801 gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag
     4861 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     4921 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     4981 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     5041 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     5101 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     5161 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     5221 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     5281 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     5341 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     5401 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     5461 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     5521 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     5581 tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc
     5641 tgaagtcagc cccatacgat ataagttgtg aagttcctat tctctagaaa gtataggaac
     5701 ttc
//
