LOCUS       sequence-252226-       13139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Azizoglu A, Brent R, Rudolf F
  TITLE     A precisely adjustable, variation-suppressed eukaryotic 
            transcriptional controller to enable genetic discovery.
  JOURNAL   Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549.
  PUBMED    34342575
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-08-3"; volume: "10. pii: 69549. doi"; pages: " 
            10.7554/eLife.69549"
COMMENT     Sequence Label: FRP2372
FEATURES             Location/Qualifiers
     source          1..13139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..18)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1..17)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10..32)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        134..373
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             374..4552
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        4949..5053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5054..5122,5123..5914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5272..5291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6093..6681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6582..6601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6835..6852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7226..7242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7231..7253
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7250..7266
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7250..7266
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7287..7305
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(7391..7580)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(7623..7643)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(9077..9326)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(11638..11658)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     12184..12203
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(13114..13133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13115..13133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
       61 ccttgcagca catccgatcg agaggcatcg cacagtttta gcgaggaaaa ctcttcaata
      121 gttttgccag cggaattgca cttgcaatta cataaaaaat tccggcggtt tttcgcgtgt
      181 gactcaatgt cgaaatacct gcctaatgaa catgaacatc gcccaaatgt atttgaagac
      241 ccgctgggag aagttcaaga tatataagta acaagcagcc aatagtataa aaaaaaatct
      301 gagtttatta cctttcctgg aatttcagtg aaaaactgct aattatagag acatatcaca
      361 gagttactca ctaatgacta acgaaaaggt ctggatagag aagttggata atccaactct
      421 ttcagtgtta ccacatgact ttttacgccc acaacaagaa ccttatacga aacaagctac
      481 atattcgtta cagctacctc agctcgatgt gcctcatgat agtttttcta acaaatacgc
      541 tgtcgctttg agtgtatggg ctgcattgat atatagagta actggtgacg atgatattgt
      601 tctttatatt gcgaataaca aaatcttaag attcaatatt caaccaacgt ggtcatttaa
      661 tgagctgtat tctacaatta acaatgagtt gaacaagctc aattctattg aggccaattt
      721 ttcctttgac gagctagctg aaaaaattca aagttgccaa gacttggaaa ggacccctca
      781 gttgttccgt ttggcctttt tggaaaacca agatttcaaa ttagacgagt tcaagcatca
      841 tttagtggac tttgctttga atttggatac cagtaataat gcgcatgttt tgaacttaat
      901 ttataacagc ttactgtatt cgaatgaaag agtaaccatt gttgcggacc aatttactca
      961 atatttgact gctgcgctaa gcgatccatc caattgcata actaaaatct ctctgatcac
     1021 cgcatcatcc aaggatagtt tacctgatcc aactaagaac ttgggctggt gcgatttcgt
     1081 ggggtgtatt cacgacattt tccaggacaa tgctgaagcc ttcccagaga gaacctgtgt
     1141 tgtggagact ccaacactaa attccgacaa gtcccgttct ttcacttatc gcgacatcaa
     1201 ccgcacttct aacatagttg cccattattt gattaaaaca ggtatcaaaa gaggtgatgt
     1261 agtgatgatc tattcttcta ggggtgtgga tttgatggta tgtgtgatgg gtgtcttgaa
     1321 agctggcgca accttttcag ttatcgaccc tgcatatccc ccagccagac aaaccattta
     1381 cttaggtgtt gctaaaccac gtgggttgat tgttattaga gctgctggac aattggatca
     1441 attagtagaa gattacatca atgatgaatt ggagattgtt tcaagaatca attccatcgc
     1501 tattcaagaa aatggtacta ttgaaggtgg caaattggac aatggcgagg atgttttggc
     1561 tccatacgat cactacaaag acaccagaac aggtgttgta gttggaccag attccaaccc
     1621 aaccctatct ttcacatctg gttccgaagg tattcctaag ggtgttcttg gtagacattt
     1681 ttccttggct tattatttca attggatgtc caaaaggttc aacttaacag aaaatgataa
     1741 attcacaatg ctgagcggta ttgcacatga tccaattcaa agagatatgt ttacaccatt
     1801 atttttaggt gcccaattgt atgtccctac tcaagatgat attggtacac cgggccgttt
     1861 agcggaatgg atgagtaagt atggttgcac agttacccat ttaacacctg ccatgggtca
     1921 attacttact gcccaagcta ctacaccatt ccctaagtta catcatgcgt tctttgtggg
     1981 tgacatttta acaaaacgtg attgtctgag gttacaaacc ttggcagaaa attgccgtat
     2041 tgttaatatg tacggtacta ctgaaacaca gcgtgcagtt tcttatttcg aagttaaatc
     2101 aaaaaatgac gatccaaact ttttgaaaaa attgaaagat gtcatgcctg ctggtaaagg
     2161 tatgttgaac gttcagctat tagttgttaa caggaacgac cgtactcaaa tatgtggtat
     2221 tggcgaaata ggtgagattt atgttcgtgc aggtggtttg gccgaaggtt atagaggatt
     2281 accagaattg aataaagaaa aatttgtgaa caactggttt gttgaaaaag atcactggaa
     2341 ttatttggat aaggataatg gtgaaccttg gagacaattc tggttaggtc caagagatag
     2401 attgtacaga acgggtgatt taggtcgtta tctaccaaac ggtgactgtg aatgttgcgg
     2461 tagggctgat gatcaagtta aaattcgtgg gttcagaatc gaattaggag aaatagatac
     2521 gcacatttcc caacatccat tggtaagaga aaacattact ttagttcgca aaaatgccga
     2581 caatgagcca acattgatca catttatggt cccaagattt gacaagccag atgacttgtc
     2641 taagttccaa agtgatgttc caaaggaggt tgaaactgac cctatagtta agggcttaat
     2701 cggttaccat cttttatcca aggacatcag gactttctta aagaaaagat tggctagcta
     2761 tgctatgcct tccttgattg tggttatgga taaactacca ttgaatccaa atggtaaagt
     2821 tgataagcct aaacttcaat tcccaactcc caagcaatta aatttggtag ctgaaaatac
     2881 agtttctgaa actgacgact ctcagtttac caatgttgag cgcgaggtta gagacttatg
     2941 gttaagtata ttacctacca agccagcatc tgtatcacca gatgattcgt ttttcgattt
     3001 aggtggtcat tctatcttgg ctaccaaaat gatttttacc ttaaagaaaa agctgcaagt
     3061 tgatttacca ttgggcacaa ttttcaagta tccaacgata aaggcctttg ccgcggaaat
     3121 tgacagaatt aaatcatcgg gtggatcatc tcaaggtgag gtcgtcgaaa atgtcactgc
     3181 aaattatgcg gaagacgcca agaaattggt tgagacgcta ccaagttcgt acccctctcg
     3241 tgaatatttt gttgaaccta atagtgccga aggaaaaaca acaattaatg tgtttgttac
     3301 tggtgtcaca ggatttctgg gctcctacat ccttgcagat ttgttaggac gttctccaaa
     3361 gaactacagt ttcaaagtgt ttgcccacgt cagggccaag gatgaagaag ctgcatttgc
     3421 aagattacaa aaggcaggta tcacctatgg tacttggaac gaaaaatttg cctcaaatat
     3481 taaagttgta ttaggcgatt tatctaaaag ccaatttggt ctttcagatg agaagtggat
     3541 ggatttggca aacacagttg atataattat ccataatggt gcgttagttc actgggttta
     3601 tccatacgcc aaattgagag atccaaatgt tatttcaact atcaatgtta tgagcttagc
     3661 cgccgtcggc aagccaaagt tctttgactt tgtttcctcc acttctactc ttgacactga
     3721 atactacttt aatttgtcag ataaacttgt tagcgaaggg aagccaggca ttttagaatc
     3781 agacgattta atgaactctg caagcgggct cactggtgga tatggtcagt ccaaatgggc
     3841 tgctgagtac atcattagac gtgcaggtga aaggggccta cgtgggtgta ttgtcagacc
     3901 aggttacgta acaggtgcct ctgccaatgg ttcttcaaac acagatgatt tcttattgag
     3961 atttttgaaa ggttcagtcc aattaggtaa gattccagac atcgaaaatt ccgtgaatat
     4021 ggttccagta gatcatgttg ctcgtgttgt tgttgctacg tctttgaatc ctcccaaaga
     4081 aaatgaattg gccgttgctc aagtaacggg tcacccaaga atattattca aagactactt
     4141 gtatacttta cacgattatg gttacgatgt cgaaatcgaa agctattcta aatggaagaa
     4201 atcattggag gcgtctgtta ttgacaggaa tgaagaaaat gcgttgtatc ctttgctaca
     4261 catggtctta gacaacttac ctgaaagtac caaagctccg gaactagacg ataggaacgc
     4321 cgtggcatct ttaaagaaag acaccgcatg gacaggtgtt gattggtcta atggaatagg
     4381 tgttactcca gaagaggttg gtatatatat tgcattttta aacaaggttg gatttttacc
     4441 tccaccaact cataatgaca aacttccact gccaagtata gaactaactc aagcgcaaat
     4501 aagtctagtt gcttcaggtg ctggtgctcg tggaagctcc gcagcagctt aaggttgagc
     4561 attacgtatg atatgcatat gtccatgtac aataattaaa tatgaattag gagaaagact
     4621 tagcttcttt tcgggtgatg tcacttaaaa actccgagaa taatatataa taagagaata
     4681 aaatattagt tattgaataa gaactgtaaa tcggctggcg ttagtctgct aatggcagct
     4741 tcatcttggt ttattgtagc atgaatcata tttgcctttt tttcctgtaa ttcaatgatt
     4801 cttgcttcta tactatcctc aatgcaaaac cttgtgatct tcacaggcgc gccaattaat
     4861 taatagcagt tcgcaaaatc gttcgcttcg caaatgttgc taatgccgcg ttctttcgac
     4921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5041 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5101 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5161 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5221 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5281 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5341 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5401 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5461 tctgacaacc atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5521 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5581 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5641 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5701 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5761 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5821 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5881 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5941 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6001 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6061 cgtaggttta aacaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6841 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6901 ccgattcatt aatgcctgca ggatggcgcg cctgaaattt acaaattgtt cgatttcaca
     6961 gcggacggtg ttgcgtcaag ggctgaaaag acaatcaatt actacaaagg aaagcagttg
     7021 ctttctccta tgggaagagc tttctaagtc tgaagaagta aacagttctt tgctatttca
     7081 cacttcctgg ttgatggtca cttgctgcct gaaatatata tatatgtatg acatatctac
     7141 ttgttttctt ttttgtgcct ttgttacgtc tatattcatt gaaactgatt attcgatttt
     7201 cttcttgctg accgcttctc agctgttgtg agcggataac aatttcacac aggaaacagc
     7261 tatgaccatg attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctgggt
     7321 acaagtgaca atatatatat atatatataa taacgacatc gttatctgta aatacggccc
     7381 aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt ataatgttac
     7441 atgcgtacac gcgtctgtac agaaaaaaaa gaaaaatttg aaatataaat aacgttctta
     7501 atactaacat aactataaaa aaataaatag ggacctagac ttcaggttgt ctaactcctt
     7561 ccttttcggt tagagcggat gtggggggag ggcgtgaatg taagcgtgac ataactaatt
     7621 caaacctttc tcttcttctt aggggcagat tcacacttca attgtttctc caaaccgcag
     7681 atgatcaatt ccaaaccaaa caaaaaagct ggttcagcac cttgatgatc aaacaactca
     7741 atggcttgtc tcaataatgg tggcatagaa tcagtagttg gagtttccct ttcttctttg
     7801 gcgacttgat gttcttgatc ttccaaaaca caacccaagg taaaatgacc aacagcagac
     7861 aaagcataca aggcgttttc taaagagaaa ccttgttgac acaagaaagc caactggttt
     7921 tccaaagttt cgtattgctt ttcagttggt ctagtaccca agtgaacttt agcaccatct
     7981 ctatgagaca acaaagcaca tctgaaagac ttggcgttgt ttctcaagaa atcttgccat
     8041 gattcacctt ccaatggaca gaaatgggta tgatgtctat ccaacatttc aatggccaaa
     8101 gcgtccaaca aagctctttt gttcttaaca tgccagtaca aggtaggttg ttcaacaccc
     8161 aatttttgag ccaactttct agtggtcaaa ccttcaatac caacttcgtt caacaactcc
     8221 aaggcagagt taataacctt ggacttgtcc aacctggaca ttttgtttgt ttatgtgtgt
     8281 ttattcgaaa ctaagttctt ggtgttttaa aactaaaaaa aagactaact ataaaagtag
     8341 aatttaagaa gtttaagaaa tagatttaca gaattactct atcaatgata gatatttata
     8401 tctatcaatg atagataggg gaataatttc agggaactgg tttcaacctt ttttttcagc
     8461 tttttccaaa tcagagagag cagaaggtaa tagaaggtgt aagaaaatga gatagataca
     8521 tgcgtgggtc aattgccttg tgtcatcatt tactccaggc aggttgcatc actccattga
     8581 ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct gtagttgcgc taagagaatg
     8641 gacctatgaa ctgatggttg gtgaagaaaa caatattttg gtgctgggat tctttttttt
     8701 agtggatgct ctatcaatga tagagctcca ttatatttag tggatgctct atcaatgata
     8761 gatcacccag acactctatc aatgatagat tctgtgtaac ccgcccccta ttttgggcat
     8821 gtacgggtta cagcagaatt aaaattagtg gatgctctat caatgataga tcacccagac
     8881 actctatcaa tgatagaggc taattttttg actaaataaa gttaggaaaa tcactactat
     8941 taattattta cgtattcttt gaaatggcag tattgataat gataaactcg aactgtctag
     9001 aaagcagacg ctactaagga aaacaacaac ggaagcgatg atgagagcga cgatgagagt
     9061 gtaaactgcg aaccttgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg
     9121 ttttcagtat aatgttacat gcgtacacgc gtttgtacag aaaaaaaaga aaaatttgaa
     9181 atataaataa cgttcttaat actaacataa ctattaaaaa aaataaatag ggacctagac
     9241 ttcaggttgt ctaactcctt ccttttcggt tagagcggat gtgggaggag ggcgtgaatg
     9301 taagcgtgac ataactaatt acatgatata gacaaaggaa aaggggcctg tctcgagtta
     9361 atttggcgct atttttttat acttccaaat ccttgcttta caatcaccgc taccagtagc
     9421 aaaaacgtta tattctggac ccagcggaga cccgtttgcc acagccacag atataactga
     9481 attcctatga ccttgcaaca tcaataacgg attgccggat ttcttatccc aaaacaggac
     9541 accacgatct ttggaaccgg acaagatgta ctcatcattt tgtgtggtgg ccacggacaa
     9601 tacaaagtct ttatgcccga tatacgtaac ttcacaagtg ccggaatttg gagttttcga
     9661 atcgctcttg ttgtttgcat tctgcaaatt ccagagctta acagatctat ctaatgagcc
     9721 ggatacaacg ctttgtccat ctctagtgaa gacaacgcta taaacagagt ccttgtggcc
     9781 tgtaccggat tcgttttccg aatctagtct ttccaccaag aatccggtct cggaatccca
     9841 aactctcaca gcacgatcta gagaaccagc agcgatgtat ttaccatcac ctggtgatac
     9901 agcgacggtg gtaacaccat cttcaatgga taaagtcaat gaacactggc ctgtacgtaa
     9961 gtcccaaata cgaacggtac ggtcaccaga accggagact aatttgtcac ctgagggaaa
    10021 gtagtccaat gaataaatat cttgttcgtg gccttgaaga atcataacaa tctttctatt
    10081 ttcaatatcc caaattctaa tcagtctgtc ttcagcacct gttgccaaaa atttcccatc
    10141 tggagaaaaa cacactgaac ggatatacaa gtcggatgat ggggaagacg aagtgttcag
    10201 gttttccaca tcttttgcca attctgctgc cgaagtcatc gctgtggtag taattgtggt
    10261 ggtagtagtg gttgtcattg tattgttatc cgtggacgtg gtggtgttat tttcagtgat
    10321 cgaatttcga tggttattgg cagcagaatc gtcagataga cgggccacca gagaaccatc
    10381 tgaaacgcga tacacttgag tagttttgtt gcagcctgtg gctaagtatt caccatcgtt
    10441 actgaacttc acgcaacaaa caactgaagt atgatccaaa gatttgtgta actcaacgtc
    10501 aatttctctt ggtagtgccg ggttgtataa aatataataa tcatttgttt gcttcttcag
    10561 agcatcggga acagactggg aatctagatc caaaaggaaa ggtgggatag gtttagagtg
    10621 gtttgctctt tgattataag ggactaagta gtgatcctgg tgcaaactag ccggggtggc
    10681 gtcttcctcc ttaggtttga tttcagtttc agttgcggtg gtagtggtgg ccgtggtggc
    10741 ggatccggtg tcatttatct tcgaggtatt attattttcc ggttcagttt ctttcaacgt
    10801 agattcaggt gccttgacag agggtaaagt agtggtctct ttagaagtag gagatccgtt
    10861 gatggctgtg ttactcaatg gtgccacgga aacctgggga ggtggttgct gttgttgaag
    10921 ttgctgctgt tgcagttgct gttgtggtag ttgttgttgg gcgtttgagg acaccacagg
    10981 caaagtggtg gtaggcaact gtgagccaac cagattagga cggctagctt gtactgggaa
    11041 ggccgatggc gaaccagtag ttgtgtttgc tgctggagtg gcggtggccg aagtagtagc
    11101 cggtggttgt tgcgcaactg gaacagatgc agatgcagcg gctagctgct gctgttgctg
    11161 ttgtaaatgc tgctggacct gttgctgctg ttgctgttgc cgctgttgct ggacggtcaa
    11221 agatgcaatt tgatggtctc tttgctccag ccctagtttc aagtgcttga tctcttcttc
    11281 gtacgcgtcc ttcattttcc tgtgagttag ttccagttcg tagacggtgt ttcttatctg
    11341 ctgcatctca gccagctgct ggttcatttt gaaatcgtaa tccttttggt tttgaagacg
    11401 gtaggtattt gcctcttgtg agacttggag aaactcctgt ctgatggcat cgagaagctc
    11461 attcagctta ttctgcgtat tcgaaacgct ggcagtcatc gcctttgccg ctgcctcctt
    11521 cgcagccgct tccttcgcgg ccgcctcctt tgcggccgcc tctgcttcta gcgccttggc
    11581 agcagcttcc ttggccgcag cctcttttgc tgccgcttcc tttgcggcgg cttctgccac
    11641 ctttctcttt ttcttggggg cactttcaca tttaagttgt ttttctaatc cgcaaatgat
    11701 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
    11761 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
    11821 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
    11881 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
    11941 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
    12001 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
    12061 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
    12121 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
    12181 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
    12241 gctgttaatc actttacttt tatctaatct ggacatgaat tcctgcagcc cgggggatcc
    12301 ggtaattgga caaataaata cgtgtattaa gtttggattc tttggtagat agccaatcga
    12361 gggaacaatt aaaaaaaaga aagaaataaa agacaagaag aagagcgaaa aaggggaaag
    12421 tgatgaggaa aagaacaata gatagatcca gaagatgatg gtaacgcgca aacactaccg
    12481 ctatatatac ctacaaaact ctcattctct catttcctgc ttcgttcatt tcgagtttcc
    12541 aagggtatgg tacggtgcta tctgcccgtg cttcccctcc tttgctttgc taagaaaaat
    12601 tttcttttgt cagcagcaac aacacgctac gctctgcgct gtgctacgaa gtggtctctg
    12661 tggcaacggg gacattgtac ccatgcccgc tcgcagggag gtctgggtgt ggggcgtgtg
    12721 cgacctcgtt gccatggcga tcgcgcgtgg gtgtggtctt tccccaaacg gttgcccgtt
    12781 gctccggatt tcgcatagtt gtcgcgtaaa caaaaaacac gagagagggc ggacggcgct
    12841 gaatagtgga agatcgcgcg atgtcaagta aataagcttg tatttgtatt tgtatgtgtt
    12901 tatgtgtttg tgtggttgag aattttattt tcctcctagt tttttctttt ttgagtgcgg
    12961 aggggcagtg ctctaaactt tgcctgcttc ttgttcgact tctagagcca ccgcggtgga
    13021 gctccaattc gccctatagg cggccgcgaa aagaaatata gtcttcaagg ttatattata
    13081 caggtttata tattattctg tttctccaat tcgccctata gtgagtcgta ttacaattc
//
