LOCUS       sequence-252227-       10900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Azizoglu A, Brent R, Rudolf F
  TITLE     A precisely adjustable, variation-suppressed eukaryotic 
            transcriptional controller to enable genetic discovery.
  JOURNAL   Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549.
  PUBMED    34342575
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-08-3"; volume: "10. pii: 69549. doi"; pages: " 
            10.7554/eLife.69549"
COMMENT     Sequence Label: FRP2374
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(954..973)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             1499..1519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3831..4080
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             5514..5534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5577..5766
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5852..5870)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5891..5907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5891..5907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5904..5926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5915..5931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6484..6501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6655..7243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6735..6754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7422..8213,8214..8282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8045..8064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8283..8387)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8528..8878
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     CDS             8879..10213
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     primer_bind     join(10886..10900,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(10900,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gagaaacaga
       61 ataatatata aacctgtata atataacctt gaagactata tttcttttcg cggccgccta
      121 tagggcgaat tggagctcca ccgcggtggc tctagaagtc gaacaagaag caggcaaagt
      181 ttagagcact gcccctccgc actcaaaaaa gaaaaaacta ggaggaaaat aaaattctca
      241 accacacaaa cacataaaca catacaaata caaatacaag cttatttact tgacatcgcg
      301 cgatcttcca ctattcagcg ccgtccgccc tctctcgtgt tttttgttta cgcgacaact
      361 atgcgaaatc cggagcaacg ggcaaccgtt tggggaaaga ccacacccac gcgcgatcgc
      421 catggcaacg aggtcgcaca cgccccacac ccagacctcc ctgcgagcgg gcatgggtac
      481 aatgtccccg ttgccacaga gaccacttcg tagcacagcg cagagcgtag cgtgttgttg
      541 ctgctgacaa aagaaaattt ttcttagcaa agcaaaggag gggaagcacg ggcagatagc
      601 accgtaccat acccttggaa actcgaaatg aacgaagcag gaaatgagag aatgagagtt
      661 ttgtaggtat atatagcggt agtgtttgcg cgttaccatc atcttctgga tctatctatt
      721 gttcttttcc tcatcacttt cccctttttc gctcttcttc ttgtctttta tttctttctt
      781 ttttttaatt gttccctcga ttggctatct accaaagaat ccaaacttaa tacacgtatt
      841 tatttgtcca attaccggat cccccgggct gcaggaattc atgtccagat tagataaaag
      901 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac
      961 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa
     1021 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt
     1081 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag
     1141 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga
     1201 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact
     1261 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga
     1321 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc
     1381 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt
     1441 attcggcctt gaattgatca tttgcggatt agaaaaacaa cttaaatgtg aaagtgcccc
     1501 caagaaaaag agaaaggtgg cagaagccgc cgcaaaggaa gcggcagcaa aagaggctgc
     1561 ggccaaggaa gctgctgcca aggcgctaga agcagaggcg gccgcaaagg aggcggccgc
     1621 gaaggaagcg gctgcgaagg aggcagcggc aaaggcgatg actgccagcg tttcgaatac
     1681 gcagaataag ctgaatgagc ttctcgatgc catcagacag gagtttctcc aagtctcaca
     1741 agaggcaaat acctaccgtc ttcaaaacca aaaggattac gatttcaaaa tgaaccagca
     1801 gctggctgag atgcagcaga taagaaacac cgtctacgaa ctggaactaa ctcacaggaa
     1861 aatgaaggac gcgtacgaag aagagatcaa gcacttgaaa ctagggctgg agcaaagaga
     1921 ccatcaaatt gcatctttga ccgtccagca acagcggcaa cagcaacagc agcaacaggt
     1981 ccagcagcat ttacaacagc aacagcagca gctagccgct gcatctgcat ctgttccagt
     2041 tgcgcaacaa ccaccggcta ctacttcggc caccgccact ccagcagcaa acacaactac
     2101 tggttcgcca tcggccttcc cagtacaagc tagccgtcct aatctggttg gctcacagtt
     2161 gcctaccacc actttgcctg tggtgtcctc aaacgcccaa caacaactac cacaacagca
     2221 actgcaacag cagcaacttc aacaacagca accacctccc caggtttccg tggcaccatt
     2281 gagtaacaca gccatcaacg gatctcctac ttctaaagag accactactt taccctctgt
     2341 caaggcacct gaatctacgt tgaaagaaac tgaaccggaa aataataata cctcgaagat
     2401 aaatgacacc ggatccgcca ccacggccac cactaccacc gcaactgaaa ctgaaatcaa
     2461 acctaaggag gaagacgcca ccccggctag tttgcaccag gatcactact tagtccctta
     2521 taatcaaaga gcaaaccact ctaaacctat cccacctttc cttttggatc tagattccca
     2581 gtctgttccc gatgctctga agaagcaaac aaatgattat tatattttat acaacccggc
     2641 actaccaaga gaaattgacg ttgagttaca caaatctttg gatcatactt cagttgtttg
     2701 ttgcgtgaag ttcagtaacg atggtgaata cttagccaca ggctgcaaca aaactactca
     2761 agtgtatcgc gtttcagatg gttctctggt ggcccgtcta tctgacgatt ctgctgccaa
     2821 taaccatcga aattcgatca ctgaaaataa caccaccacg tccacggata acaatacaat
     2881 gacaaccact actaccacca caattactac cacagcgatg acttcggcag cagaattggc
     2941 aaaagatgtg gaaaacctga acacttcgtc ttccccatca tccgacttgt atatccgttc
     3001 agtgtgtttt tctccagatg ggaaattttt ggcaacaggt gctgaagaca gactgattag
     3061 aatttgggat attgaaaata gaaagattgt tatgattctt caaggccacg aacaagatat
     3121 ttattcattg gactactttc cctcaggtga caaattagtc tccggttctg gtgaccgtac
     3181 cgttcgtatt tgggacttac gtacaggcca gtgttcattg actttatcca ttgaagatgg
     3241 tgttaccacc gtcgctgtat caccaggtga tggtaaatac atcgctgctg gttctctaga
     3301 tcgtgctgtg agagtttggg attccgagac cggattcttg gtggaaagac tagattcgga
     3361 aaacgaatcc ggtacaggcc acaaggactc tgtttatagc gttgtcttca ctagagatgg
     3421 acaaagcgtt gtatccggct cattagatag atctgttaag ctctggaatt tgcagaatgc
     3481 aaacaacaag agcgattcga aaactccaaa ttccggcact tgtgaagtta cgtatatcgg
     3541 gcataaagac tttgtattgt ccgtggccac cacacaaaat gatgagtaca tcttgtccgg
     3601 ttccaaagat cgtggtgtcc tgttttggga taagaaatcc ggcaatccgt tattgatgtt
     3661 gcaaggtcat aggaattcag ttatatctgt ggctgtggca aacgggtctc cgctgggtcc
     3721 agaatataac gtttttgcta ctggtagcgg tgattgtaaa gcaaggattt ggaagtataa
     3781 aaaaatagcg ccaaattaac tcgagacagg ccccttttcc tttgtctata tcatgtaatt
     3841 agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg
     3901 agttagacaa cctgaagtct aggtccctat ttattttttt taatagttat gttagtatta
     3961 agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt
     4021 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
     4081 aaggttcgca gtttacactc tcatcgtcgc tctcatcatc gcttccgttg ttgttttcct
     4141 tagtagcgtc tgctttctag acagttcgag tttatcatta tcaatactgc catttcaaag
     4201 aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa aaattagcct
     4261 ctatcattga tagagtgtct gggtgatcta tcattgatag agcatccact aattttaatt
     4321 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatct atcattgata
     4381 gagtgtctgg gtgatctatc attgatagag catccactaa atataatgga gctctatcat
     4441 tgatagagca tccactaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac
     4501 catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca
     4561 aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag
     4621 gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct
     4681 ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct
     4741 atctatcatt gatagatata aatatctatc attgatagag taattctgta aatctatttc
     4801 ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa aacaccaaga
     4861 acttagtttc gaataaacac acataaacaa acaaaatgtc caggttggac aagtccaagg
     4921 ttattaactc tgccttggag ttgttgaacg aagttggtat tgaaggtttg accactagaa
     4981 agttggctca aaaattgggt gttgaacaac ctaccttgta ctggcatgtt aagaacaaaa
     5041 gagctttgtt ggacgctttg gccattgaaa tgttggatag acatcatacc catttctgtc
     5101 cattggaagg tgaatcatgg caagatttct tgagaaacaa cgccaagtct ttcagatgtg
     5161 ctttgttgtc tcatagagat ggtgctaaag ttcacttggg tactagacca actgaaaagc
     5221 aatacgaaac tttggaaaac cagttggctt tcttgtgtca acaaggtttc tctttagaaa
     5281 acgccttgta tgctttgtct gctgttggtc attttacctt gggttgtgtt ttggaagatc
     5341 aagaacatca agtcgccaaa gaagaaaggg aaactccaac tactgattct atgccaccat
     5401 tattgagaca agccattgag ttgtttgatc atcaaggtgc tgaaccagct tttttgtttg
     5461 gtttggaatt gatcatctgc ggtttggaga aacaattgaa gtgtgaatct gcccctaaga
     5521 agaagagaaa ggtttgaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc
     5581 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
     5641 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
     5701 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
     5761 tcgaaggctt taatttgggc cgtatttaca gataacgatg tcgttattat atatatatat
     5821 atatattgtc acttgtaccc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
     5881 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca acagctgtca
     5941 gatatagtcg gattgccctt ttaagcaatc aatagtgttt tatttgcaac aatgtcgtca
     6001 tagtttaata tgtcctataa gatgttaact tgctcaacat tcaacaaagt ttggtctctt
     6061 ggcccagttg gttaaggcac cgtgctaata acgcggggat cagcggttcg atcccgctag
     6121 agaccattta tttttaaatt cgaccgtctg acagacggtg cacttattac ttaatttttt
     6181 ttttttttta atcctcgtta aactgagtac ataatcagca acaatataat ataaaagttt
     6241 tgctttttta tgtacgtaca ataggggtac ttaaataaat aaataaacaa taaaacaatc
     6301 aataaataaa taatatacga tcataaatta cgagatatat ttcatagtct tgacttctcc
     6361 agccgaacgt cattaaattt aaggcagaaa agataaacaa gggcgcgcca gagcctgcag
     6421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7081 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7261 ttgtttaaac ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7861 ccgatggttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8401 aaagtgccac ctgacgtcga aagaacgcgg cattagcaac atttgcgaag cgaacgattt
     8461 tgcgaactgc tattaattaa acacacacca taccttccca tatgtcctgg cgcgccctta
     8521 gctctcatta ttttttgctt tttctcttga ggtcacatga tcgcaaaatg gcaaatggca
     8581 cgtgaagctg tcgatattgg ggaactgtgg tggttggcaa atgactaatt aagttagtca
     8641 aggcgccatc ctcatgaaaa ctgtgtaaca taataaccga agtgtcgaaa aggtggcacc
     8701 ttgtccaatt gaacacgctc gatgaaaaaa ataagatata tataaggtta agtaaagcgt
     8761 ctgttagaaa ggaagttttt cctttttctt gctctcttgt cttttcatct actatttcct
     8821 tcgtgtaata cagggtcgtc agatacatag atacaattct attaccccca tccatacaat
     8881 gccatctcat ttcgatactg ttcaactaca cgccggtcaa gagaaccctg gtgacaatgc
     8941 tcacagatcc agagctgtac caatttacgc caccacttct tatgttttcg aaaactctaa
     9001 gcatggttcg caattgtttg gtttagaagt tccaggttac gtctattccc gtttccaaaa
     9061 cccaaccagt aatgttttgg aagaaagaat tgctgcttta gaaggtggtg ctgctgcttt
     9121 ggctgtttcc tccggtcaag ccgctcaaac ccttgccatc caaggtttgg cacacactgg
     9181 tgacaacatc gtttccactt cttacttata cggtggtact tataaccagt tcaaaatctc
     9241 gttcaaaaga tttggtatcg aggctagatt tgttgaaggt gacaatccag aagaatttga
     9301 aaaggtcttt gatgaaagaa ccaaggctgt ttatttggaa accattggta atccaaagta
     9361 caatgttccg gattttgaaa aaattgttgc aattgctcac aaacacggta ttccagttgt
     9421 cgttgacaac acatttggtg ccggtggtta cttctgtcag ccaattaaat acggtgctga
     9481 tattgtaaca cattctgcta ccaaatggat tggtggtcat ggtactacta tcggtggtat
     9541 tattgttgac tctggtaagt tcccatggaa ggactaccca gaaaagttcc ctcaattctc
     9601 tcaacctgcc gaaggttatc acggtactat ctacaatgaa gcctacggta acttggcata
     9661 catcgttcat gttagaactg aactattaag agatttgggt ccattgatga acccatttgc
     9721 ctctttcttg ctactacaag gtgttgaaac attatctttg agagctgaaa gacacggtga
     9781 aaatgcattg aagttagcca aatggttaga acaatcccca tacgtatctt gggtttcata
     9841 ccctggttta gcatctcatt ctcatcatga aaatgctaag aagtatctat ctaacggttt
     9901 cggtggtgtc ttatctttcg gtgtaaaaga cttaccaaat gccgacaagg aaactgaccc
     9961 attcaaactt tctggtgctc aagttgttga caatttaaag ctagcctcta acttggccaa
    10021 tgttggtgat gccaagacct tagtcattgc tccatacttc actacccaca aacaattaaa
    10081 tgacaaagaa aagttggcat ctggtgttac caaggactta attcgtgtct ctgttggtat
    10141 cgaatttatt gatgacatta ttgcagactt ccagcaatct tttgaaactg ttttcgctgg
    10201 ccaaaaacca tgagtgtgcg taatgagttg taaaattatg tataaaccta ctttctctca
    10261 caagtactat acttttataa aacgaacttt attgaaatga atatcctttt tttcccttgt
    10321 tacatgtcgt gactcgtact ttgaacctaa attgttctaa catcaaagaa cagtgttaat
    10381 tcgcagtcga gaagaaaaat atggtgaaca agactcatct acttcatgag actactttac
    10441 gcctcctata aagctgtcac actggataaa tttattgtag gaccaagtta caaaagagga
    10501 tgatggaggt ttctttacaa taaagaagca catgtgtgtt aacgttttta gtatttgctt
    10561 gttatgtaaa tcaggaaaac ttcgcgggat ttggttggat gctactttcc atacaataaa
    10621 tattatagat caaaaaagcc aaattacaag taaagattag taaagctgtt gcaattccat
    10681 cgttgataaa aatgttagtt attaaatata aaagtcagaa taggtgaact tggatttaat
    10741 tgttggcatt tcgttgctgc tagaggccat aatattagat agccaggaca tactaggtct
    10801 cctcgtggta taggaatcca taaaatggaa ttggtgacga tcggatgtgc tgcaaggcga
    10861 ttaagttggg taacgccagg gttttcccag tcacgacgtt
//
