LOCUS       sequence-252228-       10729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Azizoglu A, Brent R, Rudolf F
  TITLE     A precisely adjustable, variation-suppressed eukaryotic 
            transcriptional controller to enable genetic discovery.
  JOURNAL   Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549.
  PUBMED    34342575
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-08-3"; volume: "10. pii: 69549. doi"; pages: " 
            10.7554/eLife.69549"
COMMENT     Sequence Label: FRP2370
FEATURES             Location/Qualifiers
     source          1..10729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(954..973)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             1499..1519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3831..4080
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             5514..5534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5577..5766
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5852..5870)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5891..5907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5891..5907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5904..5926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5915..5931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6190..6207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6361..6949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6441..6460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7128..7919,7920..7988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7751..7770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7989..8093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(10715..10729,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(10729,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gagaaacaga
       61 ataatatata aacctgtata atataacctt gaagactata tttcttttcg cggccgccta
      121 tagggcgaat tggagctcca ccgcggtggc tctagaagtc gaacaagaag caggcaaagt
      181 ttagagcact gcccctccgc actcaaaaaa gaaaaaacta ggaggaaaat aaaattctca
      241 accacacaaa cacataaaca catacaaata caaatacaag cttatttact tgacatcgcg
      301 cgatcttcca ctattcagcg ccgtccgccc tctctcgtgt tttttgttta cgcgacaact
      361 atgcgaaatc cggagcaacg ggcaaccgtt tggggaaaga ccacacccac gcgcgatcgc
      421 catggcaacg aggtcgcaca cgccccacac ccagacctcc ctgcgagcgg gcatgggtac
      481 aatgtccccg ttgccacaga gaccacttcg tagcacagcg cagagagtag cgtgttgttg
      541 ctgctgacaa aagaaaattt ttcttagcaa agcaaaggag gggaagcacg ggcagatagc
      601 accgtaccat acccttggaa actcgaaatg aacgaagcag gaaatgagag aatgagagtt
      661 ttgtaggtat atatagcggt agtgtttgcg cgttaccatc atcttctgga tctatctatt
      721 gttcttttcc tcatcacttt cccctttttc gctcttcttc ttgtctttta tttctttctt
      781 ttttttaatt gttccctcga ttggctatct accaaagaat ccaaacttaa tacacgtatt
      841 tatttgtcca attaccggat cccccgggct gcaggaattc atgtccagat tagataaaag
      901 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac
      961 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa
     1021 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt
     1081 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag
     1141 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga
     1201 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact
     1261 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga
     1321 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc
     1381 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt
     1441 attcggcctt gaattgatca tttgcggatt agaaaaacaa cttaaatgtg aaagtgcccc
     1501 caagaaaaag agaaaggtgg cagaagccgc cgcaaaggaa gcggcagcaa aagaggctgc
     1561 ggccaaggaa gctgctgcca aggcgctaga agcagaggcg gccgcaaagg aggcggccgc
     1621 gaaggaagcg gctgcgaagg aggcagcggc aaaggcgatg actgccagcg tttcgaatac
     1681 gcagaataag ctgaatgagc ttctagatgc catcagacag gagtttctcc aagtctcaca
     1741 agaggcaaat acctaccgtc ttcaaaacca aaaggattac gatttcaaaa tgaaccagca
     1801 gctggctgag atgcagcaga taagaaacac cgtctacgaa ctggaactaa ctcacaggaa
     1861 aatgaaggac gcgtacgaag aagagatcaa gcacttgaaa ctagggctgg agcaaagaga
     1921 ccatcaaatt gcatctttga ccgtccagca acagcggcaa cagcaacagc agcaacaggt
     1981 ccagcagcat ttacaacagc aacagcagca gctagccgct gcatctgcat ctgttccagt
     2041 tgcgcaacaa ccaccggcta ctacttcggc caccgccact ccagcagcaa acacaactac
     2101 tggttcgcca tcggccttcc cagtacaagc tagccgtcct aatctggttg gctcacagtt
     2161 gcctaccacc actttgcctg tggtgtcctc aaacgcccaa caacaactac cacaacagca
     2221 actgcaacag cagcaacttc aacaacagca accacctccc caggtttccg tggcaccatt
     2281 gagtaacaca gccatcaacg gatctcctac ttctaaagag accactactt taccctctgt
     2341 caaggcacct gaatctacgt tgaaagaaac tgaaccggaa aataataata cctcgaagat
     2401 aaatgacacc ggatccgcca ccacggccac cactaccacc gcaactgaaa ctgaaatcaa
     2461 acctaaggag gaagacgcca ccccggctag tttgcaccag gatcactact tagtccctta
     2521 taatcaaaga gcaaaccact ctaaacctat cccacctttc cttttggatc tagattccca
     2581 gtctgttccc gatgctctga agaagcaaac aaatgattat tatattttat acaacccggc
     2641 actaccaaga gaaattgacg ttgagttaca caaatctttg gatcatactt cagttgtttg
     2701 ttgcgtgaag ttcagtaacg atggtgaata cttagccaca ggctgcaaca aaactactca
     2761 agtgtatcgc gtttcagatg gttctctggt ggcccgtcta tctgacgatt ctgctgccaa
     2821 taaccatcga aattcgatca ctgaaaataa caccaccacg tccacggata acaatacaat
     2881 gacaaccact actaccacca caattactac cacagcgatg acttcggcag cagaattggc
     2941 aaaagatgtg gaaaacctga acacttcgtc ttccccatca tccgacttgt atatccgttc
     3001 agtgtgtttt tctccagatg ggaaattttt ggcaacaggt gctgaagaca gactgattag
     3061 aatttgggat attgaaaata gaaagattgt tatgattctt caaggccacg aacaagatat
     3121 ttattcattg gactactttc cctcaggtga caaattagtc tccggttctg gtgaccgtac
     3181 cgttcgtatt tgggacttac gtacaggcca gtgttcattg actttatcca ttgaagatgg
     3241 tgttaccacc gtcgctgtat caccaggtga tggtaaatac atcgctgctg gttctctaga
     3301 tcgtgctgtg agagtttggg attccgagac cggattcttg gtggaaagac tagattcgga
     3361 aaacgaatcc ggtacaggcc acaaggactc tgtttatagc gttgtcttca ctagagatgg
     3421 acaaagcgtt gtatccggct cattagatag atctgttaag ctctggaatt tgcagaatgc
     3481 aaacaacaag agcgattcga aaactccaaa ttccggcact tgtgaagtta cgtatatcgg
     3541 gcataaagac tttgtattgt ccgtggccac cacacaaaat gatgagtaca tcttgtccgg
     3601 ttccaaagat cgtggtgtcc tgttttggga taagaaatcc ggcaatccgt tattgatgtt
     3661 gcaaggtcat aggaattcag ttatatctgt ggctgtggca aacgggtctc cgctgggtcc
     3721 agaatataac gtttttgcta ctggtagcgg tgattgtaaa gcaaggattt ggaagtataa
     3781 aaaaatagcg ccaaattaac tcgagacagg ccccttttcc tttgtctata tcatgtaatt
     3841 agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg
     3901 agttagacaa cctgaagtct aggtccctat ttattttttt taatagttat gttagtatta
     3961 agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt
     4021 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
     4081 aaggttcgca gtttacactc tcatcgtcgc tctcatcatc gcttccgttg ttgttttcct
     4141 tagtagcgtc tgctttctag acagttcgag tttatcatta tcaatactgc catttcaaag
     4201 aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa aaattagcct
     4261 ctatcattga tagagtgtct gggtgatcta tcattgatag agcatccact aattttaatt
     4321 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatct atcattgata
     4381 gagtgtctgg gtgatctatc attgatagag catccactaa atataatgga gctctatcat
     4441 tgatagagca tccactaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac
     4501 catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca
     4561 aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag
     4621 gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct
     4681 ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct
     4741 atctatcatt gatagatata aatatctatc attgatagag taattctgta aatctatttc
     4801 ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa aacaccaaga
     4861 acttagtttc gaataaacac acataaacaa acaaaatgtc caggttggac aagtccaagg
     4921 ttattaactc tgccttggag ttgttgaacg aagttggtat tgaaggtttg accactagaa
     4981 agttggctca aaaattgggt gttgaacaac ctaccttgta ctggcatgtt aagaacaaaa
     5041 gagctttgtt ggacgctttg gccattgaaa tgttggatag acatcatacc catttctgtc
     5101 cattggaagg tgaatcatgg caagatttct tgagaaacaa cgccaagtct ttcagatgtg
     5161 ctttgttgtc tcatagagat ggtgctaaag ttcacttggg tactagacca actgaaaagc
     5221 aatacgaaac tttggaaaac cagttggctt tcttgtgtca acaaggtttc tctttagaaa
     5281 acgccttgta tgctttgtct gctgttggtc attttacctt gggttgtgtt ttggaagatc
     5341 aagaacatca agtcgccaaa gaagaaaggg aaactccaac tactgattct atgccaccat
     5401 tattgagaca agccattgag ttgtttgatc atcaaggtgc tgaaccagct tttttgtttg
     5461 gtttggaatt gatcatctgc ggtttggaga aacaattgaa gtgtgaatct gcccctaaga
     5521 agaagagaaa ggtttgaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc
     5581 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
     5641 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
     5701 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
     5761 tcgaaggctt taatttgggc cgtatttaca gataacgatg tcgttattat atatatatat
     5821 atatattgtc acttgtaccc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
     5881 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca acagctgtaa
     5941 tatatagtag taacctgaaa aatagtcaat gcccgtggtt catttgccaa tagaacagtc
     6001 gatgtgatca atgtacacga aagtcaatat aaaatatcca aatagccaat ggtgtcagga
     6061 cggctaaggc aaatgcaact ttgaaatact tattcggttt gcatcttggc gcgcctcttc
     6121 ctgcaggcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6961 gatcttttgt ttaaacctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7021 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7081 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7141 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7201 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7261 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7321 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7381 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7441 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7501 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7561 ggtcctccga tggttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7621 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7681 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7741 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7801 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7861 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7921 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7981 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8041 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8101 ccccgaaaag tgccacctga cgtcgaaaga acgcggcatt agcaacattt gcgaagcgaa
     8161 cgattttgcg aactgctatt aattaactct ggcgcgccta actttttgtg tggtgccctc
     8221 ctccttgtca atattaatgt taaagtgcaa ttctttttcc ttatcacgtt gagccattag
     8281 tatcaatttg cttacctgta ttcctttaca tcctcctttt tctccttctt gataaatgta
     8341 tgtagattgc gtatatagtt tcgtctaccc tatgaacata ttccattttg taatttcgtg
     8401 tcgcatatga tccgcaggct aaccggaacc tgtattattt agtttatgct acgttaaata
     8461 aagacctttc gttcacataa ctgaatgtgt aatggccttg agatttcaag cataccaagt
     8521 tggtggagac ggggtcgtta caaaagactc tttaagccaa gatttccttg acagccttgg
     8581 cgatagcatc gccaacctca gtggtagagt tagaaccacc aaggtcaccg gttctgacac
     8641 ctgcatccaa gacatttcta acagcttctt caagagccct accttcttca accaaatcca
     8701 aggataactt caacatcata gctgcagata agatggtagc aattgggtta accttgtttg
     8761 ctggtaaatc tggggcagaa ccatgacatg gttcgtacaa accgaatgcc ttgttagtgt
     8821 caggtaggga agctagagat gcagaaggta ataaacccaa agaacctgga ataacagagg
     8881 cttcatcgga gataatatca ccaaacatgt tgttggtaat aacaacaccg tttagcttag
     8941 ttggtgattt aaccaaaatc atagcagcag agtcgatcaa ttggtgctga acagttaatt
     9001 gtgggaactc agtcttgatg gtttcttcaa cagtctttct ccacaatctg gaagaggcaa
     9061 gcacgttagc cttgtcaaga gaccagattg gtaatggtgg gttttgttgc aatgccaaga
     9121 aagcagccat tcttgtaatt ctttgaactt caggaacact gtatttctca gagtcccaag
     9181 caactccgtc accttcatct tcttttcttt caccaaagta gataccacca accaattctc
     9241 taacgacgac gaaatcagta ccctttgcat attcaggctt caaaggagaa agatctagta
     9301 aagaatcaga agcaaagtta catggtctca agttggcgta tagacccaat tcctttctga
     9361 tcttcaatag accttgttct ggtctaactg cgcccgtacc ccattttgga ccaccaacag
     9421 cacctagtaa gacagcatcg gctttcttag aggcttcaag agcttcatct ggtaaaggag
     9481 tgccagtggc atcgatggca gcacccccga tcaagtgatg ttggaaattg aacttaattt
     9541 ctggacggac ttcagcaatg gcattcaaga ccttaatagc ttcgtcagta acttctttac
     9601 cgacgtgatc acccggtagg acaacgatat tcttagacat ggtataatct gtgtagtgtg
     9661 ggatactttt tacttcttca aataggtatc aacaataaaa ataaatcgaa gcaaatgtag
     9721 gaatgcgtta aagcagatgt acttacttag agtacataga tatatttata taattcaata
     9781 tataaaagta tatgaagcat ctgatgttga acctgtcatg actctaaact ggaatgggta
     9841 gttatgcttc tgaaggtttt ccgttagaaa atgagtgcag ggatcaggcc cggaaccggt
     9901 tttagcctga aaggaaaaga aaaaaatgcg gtgattacta gcacgtgact gcgctgaatt
     9961 ggaatctacc acaagtacag cgaatggcaa gagggaaggg gaatatattg tgcctggcgg
    10021 tagcgtctgc tatatcaaat gttaaacata actaaatgca gtagtgaata gagtaatgta
    10081 tttctacatc tgtgggtagg gccaatggga gcccgatgtc tattgtacag atattttcct
    10141 tttatagtta atcaattcaa cttggaaatc attgagtcga tgtctacttg gataactgga
    10201 gccgtggcag cgtttgaaat ttgacgagtc ttcttgtttt gttcgcgcat tttcagttta
    10261 ttcaaacggg agatggcacc gaatccactg acttctctgt tcaatttgaa tggtaatagg
    10321 aagaaagtac atttcgagaa attttcgccc aacgtcgaaa tctcatggat gatttcctcg
    10381 atacaaatga caccttcatc acctaatttc tcttcaacga tattgttgtc attcaagatt
    10441 atctcttttg gttctttatc ttctggcctt tgccacatta ttctagttct cttttggatt
    10501 aaagaacgga tagatgcaag tgaaggttgt ccgataacaa tatatggtga tacaagtttc
    10561 aacaatgggt acacgtcctt ggtaagtttg atgaaaacac cagtgttcaa ctcaacaagt
    10621 ctcaaaagag ataacacttt gtaagccttt gctgggatct tcacagcgat cggatgtgct
    10681 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgtt
//
