LOCUS       sequence-252232-        2818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for Rob.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: Prob-cfp  (ColE1)
FEATURES             Location/Qualifiers
     source          1..2818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             615..626
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1399..1470
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1486..1513
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1544..2132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1624..1643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2215..2309)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2340..2818,1..316))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2451..2470)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
       61 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
      121 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
      181 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
      241 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
      301 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat catgatcccc
      361 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      421 ccttaccaga gggcgcccca gctggcaatt ccgacgtcgg tgcctaatga gtgagctaac
      481 ttacattaat tgcgttgcgc caaaatctca atacttttat ttccgtcaag ccctaaaaca
      541 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
      601 ggatccttaa ctttaaagag gagaaaggta ccgcatgact agcaaaagaa gcaaaggtga
      661 agaactgttc actggtgttg ttccaattct ggttgaactg gatggtgatg ttaatggtca
      721 caaattttct gtctctggtg agggtgaagg tgatgcaacc tacggtaaac tgaccctgaa
      781 atttatttgc actactggta aactgcctgt tccgtggcca accctggtca ctactctgac
      841 ttggggtgtt caatgctttg ctcgttaccc agatcacatg aaacagcatg actttttcaa
      901 gtctgccatg ccggaaggtt atgttcagga acgtactatc tttttcaaag atgacggtaa
      961 ctacaagacc cgtgctgaag tcaagtttga aggtgatacc ctggttaatc gtatcgagct
     1021 gaaaggtatt gattttaaag aagatggtaa cattctgggt cacaaactgg aatacaacgc
     1081 tatttctgat aatgtataca tcactgctga caaacaaaag aatggtatca aagctaattt
     1141 caaaattcgt cacaacattg aagatggtag cgttcaactg gcagaccatt atcaacaaaa
     1201 tactccaatt ggcgatggcc ctgtcctgct gccagacaac cattacctgt ccacccaatc
     1261 tcgtctgtct aaagatccga acgaaaagcg cgatcacatg gtcctgctgg agtttgtaac
     1321 cgctgctggt attaccctgg gcatggatga actgtataaa taatagggat ccaaactcga
     1381 gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1441 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1501 gggcctttct gcgtttatac ctaggggccg cgttgctggc gtttttccat aggctccgcc
     1561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1981 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgagc ttggattctc
     2221 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     2281 ggtcattact ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct
     2341 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2401 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2461 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2521 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2581 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2641 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2701 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2761 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggt
//
