LOCUS       sequence-252233-        4433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for HdeA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PhdeA-cfp
FEATURES             Location/Qualifiers
     source          1..4433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             689..700
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1473..1544
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1560..1587
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2118..3068)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3830..3924)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3955..4433,1..316))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4066..4085)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
       61 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
      121 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
      181 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
      241 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
      301 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat catgatcccc
      361 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      421 ccttaccaga gggcgcccca gctggcaatt ccgacgtcgg tgcctaatga gtgagctaac
      481 ttacattaat tgcgttgcgc cccctgctat caatctatgc caaaaacgcg tctaagaatg
      541 cagtcgattt aataaaaatt tcctaattgc agtatctgat gcatctgtaa ctcattgtat
      601 tgaaataaaa atatctgatt ttgatatttt ccatcaacat gacatataca gaaaaccagg
      661 ttataacctc agtgggatcc ttaactttaa agaggagaaa ggtaccgcat gactagcaaa
      721 agaagcaaag gtgaagaact gttcactggt gttgttccaa ttctggttga actggatggt
      781 gatgttaatg gtcacaaatt ttctgtctct ggtgagggtg aaggtgatgc aacctacggt
      841 aaactgaccc tgaaatttat ttgcactact ggtaaactgc ctgttccgtg gccaaccctg
      901 gtcactactc tgacttgggg tgttcaatgc tttgctcgtt acccagatca catgaaacag
      961 catgactttt tcaagtctgc catgccggaa ggttatgttc aggaacgtac tatctttttc
     1021 aaagatgacg gtaactacaa gacccgtgct gaagtcaagt ttgaaggtga taccctggtt
     1081 aatcgtatcg agctgaaagg tattgatttt aaagaagatg gtaacattct gggtcacaaa
     1141 ctggaataca acgctatttc tgataatgta tacatcactg ctgacaaaca aaagaatggt
     1201 atcaaagcta atttcaaaat tcgtcacaac attgaagatg gtagcgttca actggcagac
     1261 cattatcaac aaaatactcc aattggcgat ggccctgtcc tgctgccaga caaccattac
     1321 ctgtccaccc aatctcgtct gtctaaagat ccgaacgaaa agcgcgatca catggtcctg
     1381 ctggagtttg taaccgctgc tggtattacc ctgggcatgg atgaactgta taaataatag
     1441 ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
     1501 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1561 gctcaccttc gggtgggcct ttctgcgttt atacctaggg tacgggtttt gctgcccgca
     1621 aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc ttcagccggt
     1681 ttgccggctg aaagcgctat ttcttccaga attgccatga ttttttcccc acgggaggcg
     1741 tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc tgtttcaggc
     1801 tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta
     1861 gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt tcatctgtta
     1921 cattgtcgat ctgttcatgg tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg
     1981 atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt ccctttgata
     2041 tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
     2101 caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt
     2161 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca
     2221 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
     2281 gtttttctta gtccgttatg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
     2341 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
     2401 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
     2461 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
     2521 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
     2581 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
     2641 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
     2701 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
     2761 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
     2821 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
     2881 ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc
     2941 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
     3001 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
     3061 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
     3121 agtcaatgat aattactagt ccttttcccg ggtgatctgg gtatctgtaa attctgctag
     3181 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
     3241 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
     3301 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
     3361 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
     3421 aaaggcttaa gtagcaccct cgcaagctcg agcaaatcgc tgaatattcc ttttgtctcc
     3481 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
     3541 tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa
     3601 actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct
     3661 gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt
     3721 ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg
     3781 cagcggtatc atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa
     3841 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     3901 ttactggatc tatcaacagg agtccaagcg agctctcgaa ccccagagtc ccgctcagaa
     3961 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     4021 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     4081 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     4141 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     4201 atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     4261 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     4321 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     4381 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggt
//
