LOCUS       sequence-252243-        4473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for AcrAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PacrAB-cfp
FEATURES             Location/Qualifiers
     source          1..4473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(680..774)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(805..1599)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(916..935)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1526..1545
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             2012..2023
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2796..2867
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2883..2910
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3441..4391)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 ggtgatctgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
       61 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
      121 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
      181 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
      241 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
      301 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
      361 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
      421 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
      481 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
      541 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
      601 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
      661 tactgtccct agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      721 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
      781 agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
      841 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      901 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      961 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     1021 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     1081 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     1141 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     1201 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     1261 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     1321 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     1381 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     1441 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     1501 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     1561 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     1621 ctcttgatca gatcatgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     1681 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt
     1741 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgcgccagta gattgcaccg
     1801 cgcgtaacgc cagctgcttt tgcaatctcg cccagcgagg tggatgatac cccctgctgt
     1861 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
     1921 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
     1981 tatgtaccat agcacgagga tccttaactt taaagaggag aaaggtaccg catgactagc
     2041 aaaagaagca aaggtgaaga actgttcact ggtgttgttc caattctggt tgaactggat
     2101 ggtgatgtta atggtcacaa attttctgtc tctggtgagg gtgaaggtga tgcaacctac
     2161 ggtaaactga ccctgaaatt tatttgcact actggtaaac tgcctgttcc gtggccaacc
     2221 ctggtcacta ctctgacttg gggtgttcaa tgctttgctc gttacccaga tcacatgaaa
     2281 cagcatgact ttttcaagtc tgccatgccg gaaggttatg ttcaggaacg tactatcttt
     2341 ttcaaagatg acggtaacta caagacccgt gctgaagtca agtttgaagg tgataccctg
     2401 gttaatcgta tcgagctgaa aggtattgat tttaaagaag atggtaacat tctgggtcac
     2461 aaactggaat acaacgctat ttctgataat gtatacatca ctgctgacaa acaaaagaat
     2521 ggtatcaaag ctaatttcaa aattcgtcac aacattgaag atggtagcgt tcaactggca
     2581 gaccattatc aacaaaatac tccaattggc gatggccctg tcctgctgcc agacaaccat
     2641 tacctgtcca cccaatctcg tctgtctaaa gatccgaacg aaaagcgcga tcacatggtc
     2701 ctgctggagt ttgtaaccgc tgctggtatt accctgggca tggatgaact gtataaataa
     2761 tagggatcca aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
     2821 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2881 ctggctcacc ttcgggtggg cctttctgcg tttataccta gggtacgggt tttgctgccc
     2941 gcaaacgggc tgttctggtg ttgctagttt gttatcagaa tcgcagatcc ggcttcagcc
     3001 ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc cccacgggag
     3061 gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc gcctgtttca
     3121 ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca atttcatgtt
     3181 ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc tgttcatctg
     3241 ttacattgtc gatctgttca tggtgaacag ctttgaatgc accaaaaact cgtaaaagct
     3301 ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt tttccctttg
     3361 atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct tcactgatag
     3421 atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg
     3481 gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt actttgcatg
     3541 tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt
     3601 agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt tggtattttg
     3661 tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct
     3721 agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc
     3781 atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt
     3841 ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc
     3901 tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc
     3961 ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt
     4021 tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt
     4081 gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg
     4141 atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt
     4201 tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct
     4261 ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg
     4321 gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact
     4381 aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg
     4441 gctagtcaat gataattact agtccttttc ccg
//
