LOCUS       sequence-252248-        2817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for CodB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PcodB-cfp  (ColE1)
FEATURES             Location/Qualifiers
     source          1..2817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             114..125
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      898..969
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      985..1012
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1043..1631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1123..1142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1714..1808)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1839..2633)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1950..1969)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2560..2579
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gataattttt cccccacctt tttgcactca ttcatataaa aaatatattt ccccacgaaa
       61 acgattgctt tttatcttca gatgaataga atgcggcggg gatccttaac tttaaagagg
      121 agaaaggtac cgcatgacta gcaaaagaag caaaggtgaa gaactgttca ctggtgttgt
      181 tccaattctg gttgaactgg atggtgatgt taatggtcac aaattttctg tctctggtga
      241 gggtgaaggt gatgcaacct acggtaaact gaccctgaaa tttatttgca ctactggtaa
      301 actgcctgtt ccgtggccaa ccctggtcac tactctgact tggggtgttc aatgctttgc
      361 tcgttaccca gatcacatga aacagcatga ctttttcaag tctgccatgc cggaaggtta
      421 tgttcaggaa cgtactatct ttttcaaaga tgacggtaac tacaagaccc gtgctgaagt
      481 caagtttgaa ggtgataccc tggttaatcg tatcgagctg aaaggtattg attttaaaga
      541 agatggtaac attctgggtc acaaactgga atacaacgct atttctgata atgtatacat
      601 cactgctgac aaacaaaaga atggtatcaa agctaatttc aaaattcgtc acaacattga
      661 agatggtagc gttcaactgg cagaccatta tcaacaaaat actccaattg gcgatggccc
      721 tgtcctgctg ccagacaacc attacctgtc cacccaatct cgtctgtcta aagatccgaa
      781 cgaaaagcgc gatcacatgg tcctgctgga gtttgtaacc gctgctggta ttaccctggg
      841 catggatgaa ctgtataaat aatagggatc caaactcgag taaggatctc caggcatcaa
      901 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      961 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatacc
     1021 taggggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1381 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1441 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1681 actcacgtta agggattttg gtcatgagct tggattctca ccaataaaaa acgcccggcg
     1741 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     1801 caggagtcca agcgagctct cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     1861 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     1921 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     1981 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     2041 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     2101 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     2161 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     2221 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     2281 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     2341 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     2401 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     2461 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     2521 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     2581 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     2641 gatcctcatc ctgtctcttg atcagatcat gatcccctgc gccatcagat ccttggcggc
     2701 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     2761 ggcaattccg acgtcggtgc ctaatgagtg agctaactta cattaattgc gttgcgc
//
