LOCUS       sequence-252249-        3159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for OmpF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PompF-cfp  (ColE1)
FEATURES             Location/Qualifiers
     source          1..3159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             956..967
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1740..1811
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1827..1854
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1885..2473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1965..1984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2556..2650)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2681..3159,1..316))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2792..2811)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
       61 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
      121 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
      181 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
      241 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
      301 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat catgatcccc
      361 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      421 ccttaccaga gggcgcccca gctggcaatt ccgacgtcgg tgcctaatga gtgagctaac
      481 ttacattaat tgcgttgcgc caagttatct gtttgttaag tcaagcaatc tatttgcaac
      541 cccgccataa cgatcatcct gttacggaat attacattgc aacatttacg cgcaaaaact
      601 aatccgcatt cttattgcgg attagttttt tcttagctaa tagcacaatt ttcatactat
      661 tttttggcat tctggatgtc tgaaagaaga ttttgtgcca ggtcgataaa gtttccatca
      721 gaaacaaaat ttccgtttag ttaatttaaa tataaggaaa tcatataaat agattaaaat
      781 tgctgtaaat atcatcacgt ctctatggaa atatgacggt gttcacaaag ttccttaaat
      841 tttacttttg gttacatatt ttttcttttt gaaaccaaat ctttatcttt gtagcacttt
      901 cacggtagcg aaacgttagt ttgaatggaa agatgcctgc aggatcctta actttaaaga
      961 ggagaaaggt accgcatgac tagcaaaaga agcaaaggtg aagaactgtt cactggtgtt
     1021 gttccaattc tggttgaact ggatggtgat gttaatggtc acaaattttc tgtctctggt
     1081 gagggtgaag gtgatgcaac ctacggtaaa ctgaccctga aatttatttg cactactggt
     1141 aaactgcctg ttccgtggcc aaccctggtc actactctga cttggggtgt tcaatgcttt
     1201 gctcgttacc cagatcacat gaaacagcat gactttttca agtctgccat gccggaaggt
     1261 tatgttcagg aacgtactat ctttttcaaa gatgacggta actacaagac ccgtgctgaa
     1321 gtcaagtttg aaggtgatac cctggttaat cgtatcgagc tgaaaggtat tgattttaaa
     1381 gaagatggta acattctggg tcacaaactg gaatacaacg ctatttctga taatgtatac
     1441 atcactgctg acaaacaaaa gaatggtatc aaagctaatt tcaaaattcg tcacaacatt
     1501 gaagatggta gcgttcaact ggcagaccat tatcaacaaa atactccaat tggcgatggc
     1561 cctgtcctgc tgccagacaa ccattacctg tccacccaat ctcgtctgtc taaagatccg
     1621 aacgaaaagc gcgatcacat ggtcctgctg gagtttgtaa ccgctgctgg tattaccctg
     1681 ggcatggatg aactgtataa ataataggga tccaaactcg agtaaggatc tccaggcatc
     1741 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1801 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     1861 cctaggggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1921 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1981 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2041 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2101 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2161 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2221 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2281 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2341 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2401 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2461 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2521 aaactcacgt taagggattt tggtcatgag cttggattct caccaataaa aaacgcccgg
     2581 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2641 aacaggagtc caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     2701 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     2761 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     2821 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     2881 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     2941 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     3001 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     3061 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     3121 catcagccat gatggatact ttctcggcag gagcaaggt
//
