LOCUS       sequence-252250-        2902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for DnaQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PdnaQ-cfp  (ColE1)
FEATURES             Location/Qualifiers
     source          1..2902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             699..710
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1483..1554
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1570..1597
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1628..2216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1708..1727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2299..2393)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2424..2902,1..316))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2535..2554)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
       61 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
      121 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
      181 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
      241 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
      301 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat catgatcccc
      361 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      421 ccttaccaga gggcgcccca gctggcaatt ccgacgtcgg tgcctaatga gtgagctaac
      481 ttacattaat tgcgttgcgc gttatggatc cactgggtga taccctggcg gacatactgg
      541 ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg cctccagcgc gacaatagcg
      601 gccatcaact ccatacggtt gttggtggtg cgggtgtagc cagcgctaaa ggttttctcg
      661 cgtccgcgat agcgtaaaat agcgggatcc ttaactttaa agaggagaaa ggtaccgcat
      721 gactagcaaa agaagcaaag gtgaagaact gttcactggt gttgttccaa ttctggttga
      781 actggatggt gatgttaatg gtcacaaatt ttctgtctct ggtgagggtg aaggtgatgc
      841 aacctacggt aaactgaccc tgaaatttat ttgcactact ggtaaactgc ctgttccgtg
      901 gccaaccctg gtcactactc tgacttgggg tgttcaatgc tttgctcgtt acccagatca
      961 catgaaacag catgactttt tcaagtctgc catgccggaa ggttatgttc aggaacgtac
     1021 tatctttttc aaagatgacg gtaactacaa gacccgtgct gaagtcaagt ttgaaggtga
     1081 taccctggtt aatcgtatcg agctgaaagg tattgatttt aaagaagatg gtaacattct
     1141 gggtcacaaa ctggaataca acgctatttc tgataatgta tacatcactg ctgacaaaca
     1201 aaagaatggt atcaaagcta atttcaaaat tcgtcacaac attgaagatg gtagcgttca
     1261 actggcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc tgctgccaga
     1321 caaccattac ctgtccaccc aatctcgtct gtctaaagat ccgaacgaaa agcgcgatca
     1381 catggtcctg ctggagtttg taaccgctgc tggtattacc ctgggcatgg atgaactgta
     1441 taaataatag ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct
     1501 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacctaggg gccgcgttgc
     1621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2041 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2281 ttttggtcat gagcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2341 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2401 gctctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     2461 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     2521 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     2581 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     2641 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     2701 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     2761 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     2821 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     2881 actttctcgg caggagcaag gt
//
