LOCUS       sequence-252251-        3017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for Fis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: Pfis-cfp  (ColE1)
FEATURES             Location/Qualifiers
     source          1..3017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             814..825
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1598..1669
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1685..1712
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1743..2331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1823..1842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2414..2508)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2539..3017,1..316))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2650..2669)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
       61 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
      121 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
      181 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
      241 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
      301 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat catgatcccc
      361 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      421 ccttaccaga gggcgcccca gctggcaatt ccgacgtcgg tgcctaatga gtgagctaac
      481 ttacattaat tgcgttgcgc gcgaagtgcg agcaagctca caaaaggcac gtaaatttgc
      541 cgattattta cgcaaatttg cgtgccaaaa ttttcattca taaagaaaaa ttgagaactt
      601 actcaaattt ctttgagtgt aaattttagt cactattttc taatatgatg atttttatga
      661 gtaattatcg caccacgctc attttaaatg caattctttg atccatctca gaggattggt
      721 caaagtttgg cctttcatct cgtgcaaaaa atgcgtaata tacgccgcct tgcagtcaca
      781 gtatggtcat ttcttaactg gatccttaac tttaaagagg agaaaggtac cgcatgacta
      841 gcaaaagaag caaaggtgaa gaactgttca ctggtgttgt tccaattctg gttgaactgg
      901 atggtgatgt taatggtcac aaattttctg tctctggtga gggtgaaggt gatgcaacct
      961 acggtaaact gaccctgaaa tttatttgca ctactggtaa actgcctgtt ccgtggccaa
     1021 ccctggtcac tactctgact tggggtgttc aatgctttgc tcgttaccca gatcacatga
     1081 aacagcatga ctttttcaag tctgccatgc cggaaggtta tgttcaggaa cgtactatct
     1141 ttttcaaaga tgacggtaac tacaagaccc gtgctgaagt caagtttgaa ggtgataccc
     1201 tggttaatcg tatcgagctg aaaggtattg attttaaaga agatggtaac attctgggtc
     1261 acaaactgga atacaacgct atttctgata atgtatacat cactgctgac aaacaaaaga
     1321 atggtatcaa agctaatttc aaaattcgtc acaacattga agatggtagc gttcaactgg
     1381 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctgctg ccagacaacc
     1441 attacctgtc cacccaatct cgtctgtcta aagatccgaa cgaaaagcgc gatcacatgg
     1501 tcctgctgga gtttgtaacc gctgctggta ttaccctggg catggatgaa ctgtataaat
     1561 aatagggatc caaactcgag taaggatctc caggcatcaa ataaaacgaa aggctcagtc
     1621 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1681 cactggctca ccttcgggtg ggcctttctg cgtttatacc taggggccgc gttgctggcg
     1741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2161 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2401 gtcatgagct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     2461 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctct
     2521 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     2581 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     2641 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     2701 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2761 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2821 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2881 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2941 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     3001 ctcggcagga gcaaggt
//
