LOCUS       sequence-252263-       11471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generation of Tet-On RPE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 11471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA095
FEATURES             Location/Qualifiers
     source          1..11471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        295..607
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    303..321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    339..357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    374..392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    410..428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    446..464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    481..499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    517..535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     580..604
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      680..689
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5629..5750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5640..5659)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5694..5713
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      6467..6476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6473..6475,6476..6478,6479..7189)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6473..6475,6476..6478,6479..7189)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6518..6539)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6779..6798)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7126..7147
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7199..7255
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7256..7873,7874..7879,7880..7915,7916..7918,7919..7954,
                     7955..7957,7958..7993,7994..7999)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             8006..8062
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8057..8857
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8117..8136)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8727..8746
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8912..8929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8918..9142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9468..9485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9639..10227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9719..9738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10398..11189,11190..11258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11021..11040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11259..11363)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agacgagttt
      301 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      361 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      421 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
      481 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      541 tgtcgattag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
      601 gatcgcctgg agccaattcc aactcttttg ccttatacct actttccgta ccacttccta
      661 ccctcgaaag gcctctgagg ccaccatggg caagaagcag aaccgcaaga ccggcaacag
      721 caagacccag agcgccagcc ccccccccaa ggagcgcagc agcagccccg ccaccgagca
      781 gagctggatg gagaacgact tcgacgagct gcgcgaggag ggcttccgcc gcagcaacta
      841 cagcgagctg cgcgaggaca tccagaccaa gggcaaggag gtggagaact tcgagaagaa
      901 cctggaggag tgcatcaccc gcatcaccaa caccgagaag tgcctgaagg agctgatgga
      961 gctgaagacc aaggcccgcg agctgcgcga ggagtgccgc agcctgcgca gccgctgcga
     1021 ccaattggag gagcgcgtga gcgccatgga ggacgagatg aacgagatga agcgcgaggg
     1081 caagttccgc gagaagcgca tcaagcgcaa cgagcagagc ctgcaggaga tctgggacta
     1141 cgtgaagcgc cccaacctgc gcctgatcgg cgtgcccgag agcgacgtgg agaacggcac
     1201 caagctggag aacaccctgc aggacatcat ccaggagaac ttccccaacc tggcccgcca
     1261 ggccaacgtg cagatccagg agatccagcg caccccccag cgctacagca gccgccgcgc
     1321 caccccccgc cacatcatcg tgcgcttcac caaggtggag atgaaggaga agatgctgcg
     1381 cgccgcccgc gagaagggcc gcgtgaccct gaagggcaag cccatccgtc tcaccgccga
     1441 cctgagcgcc gagaccctgc aggcccgccg cgagtggggc cccatcttca acatcctgaa
     1501 ggagaagaac ttccagcccc gcatcagcta ccccgccaag ctgagcttca tcagcgaggg
     1561 cgagatcaag tacttcatcg acaagcagat gctgcgcgac ttcgtgacca cccgccccgc
     1621 cctgaaggag ctgctgaagg aggccctgaa catggagcgc aacaaccgct accagcccct
     1681 gcagaaccac gccaagatgt aaagaccatc gagactagga agaaactgca tcaactaatg
     1741 agcaaaatca ggcgcgccca tcataatgac cggcagcaac agccacatca ccatcctgac
     1801 cctgaacatc aacggcctga acagcgccat caagcgccac cgcctggcca gctggatcaa
     1861 gagccaggac cccagcgtgt gctgcatcca ggagacccac ctgacctgcc gcgacaccca
     1921 ccgcctgaag atcaagggct ggcgcaagat ctaccaggcc aacggcaagc agaagaaggc
     1981 cggcgtggcc atcctggtga gcgacaagac cgacttcaag cccaccaaga tcaagcgcga
     2041 caaggagggc cactacatca tggtgaaggg cagcatccag caggaggagc tgaccatcct
     2101 gaacatctac gcccccaaca ccggtgcccc ccgcttcatc aagcaggtgc tgagcgacct
     2161 gcagcgcgac ctggacagcc acaccctgat catgggcgac ttcaacaccc ccctgagcac
     2221 cctggaccgc agcacccgcc agaaggtgaa caaggacacc caggagctga acagcgccct
     2281 gcaccaggcc gacctgatcg acatctaccg caccctgcac cccaagagca ccgagtacac
     2341 cttcttcagc gccccccacc acacctacag caagatcgac cacatcgtgg gcagcaaggc
     2401 cctgctgagc aagtgcaagc gcaccgagat catcaccaac tacctgagcg accacagcgc
     2461 catcaagctg gagctgcgca tcaagaacct gacccagagc cgcagcacca cctggaagct
     2521 gaacaacctg ctgctgaacg actactgggt gcacaacgag atgaaggccg agatcaagat
     2581 gttcttcgaa accaacgaga acaaggacac cacctaccag aacctgtggg acgccttcaa
     2641 ggccgtgtgc cgcggcaagt tcatcgccct gaacgcctac aagcgcaagc aggagcgcag
     2701 caagatcgac accctgacca gccagctgaa ggagctggag aagcaggagc agacccacag
     2761 caaggccagc cgccgccagg agatcaccaa gatccgcgcc gagctgaagg agatcgagac
     2821 ccagaagacc ctgcagaaga tcaacgagtc gcgaagctgg ttcttcgagc gcatcaacaa
     2881 gatcgaccgc cccctggccc gcctgatcaa gaagaagcgc gagaagaacc agatcgacac
     2941 catcaagaac gacaagggcg acatcaccac cgaccccacc gagatccaga ccaccatccg
     3001 cgagtactac aagcacctgt acgccaacaa gctggagaac ctggaggaga tggacacctt
     3061 cctggacacc tacaccctgc cccgcctgaa ccaggaggag gtggagagcc tgaaccgccc
     3121 catcaccggc agcgagatcg tggccatcat caacagcctg cccaccaaga agagccccgg
     3181 ccccgacggc ttcaccgccg agttctacca gcgctacaag gaggagctgg tgcccttcct
     3241 gctgaagctg ttccagagca tcgagaagga gggcatcctg cccaacagct tctacgaggc
     3301 cagcatcatc ctgatcccca agcccggccg cgacaccacc aagaaggaga acttccgccc
     3361 catcagcctg atgaacatcg acgccaagat cctgaacaag atcctggcca accgcatcca
     3421 gcagcacatc aagaagctga tccaccacga ccaggtgggc ttcatccccg ggatgcaggg
     3481 ctggttcaac atccgcaaga gcatcaacgt gatccagcac atcaaccgcg ccaaggacaa
     3541 gaaccacatg atcatcagca tcgacgccga gaaggccttc gacaagatcc agcagccctt
     3601 catgctgaag accctgaaca agctgggcat cgacggcacc tacttcaaga tcatccgcgc
     3661 catctacgac aagcccaccg ccaacatcat cctgaacggc cagaagctgg aggccttccc
     3721 cctgaagacc ggcacgcgtc agggctgccc cctgagcccc ctgctgttca acatcgtgct
     3781 ggaggtgctg gcccgcgcca tccgccagga gaaggagatc aagggcatcc agctgggcaa
     3841 ggaggaggtg aagctgagcc tgttcgccga cgacatgatc gtgtacctgg agaaccccat
     3901 cgtgagcgcc cagaacctgc tgaagctgat cagcaacttc agcaaggtga gcggctacaa
     3961 gatcaacgtg cagaagagcc aggccttcct gtacaccaac aaccgccaga ccgagagcca
     4021 gatcatgggc gagctgccct tcaccatcgc tagcaagcgc atcaagtacc tgggcatcca
     4081 gctgacccgc gacgtgaagg acctgttcaa ggagaactac aagcccctgc tgaaggagat
     4141 caaggaggag accaacaagt ggaagaacat cccctgcagc tgggtgggcc gcatcaacat
     4201 cgtgaagatg gccatcctgc ccaaggtgat ctaccgcttc aacgccatcc ccatcaagct
     4261 gcccatgacc ttcttcaccg agctggagaa gaccaccctg aagttcatct ggaaccagaa
     4321 gcgcgcccgc atcgccaaga gcatcctgag ccagaagaac aaggccggcg gcatcaccct
     4381 gcccgacttc aagctgtact acaaggccac cgtgaccaag accgcctggt actggtacca
     4441 gaaccgcgat atcgaccagt ggaaccgcac cgagcccagc gagatcatgc cccacatcta
     4501 caactacctg atcttcgaca agcccgagaa gaacaagcag tggggcaagg acagcctgtt
     4561 caacaagtgg tgctgggaga actggctggc catctgccgc aagctgaagc tggacccctt
     4621 cctgaccccc tacaccaaga tcaacagccg ctggatcaag gacctgaacg tgaagcccaa
     4681 gaccatcaag accctggagg agaacctggg catcaccatc caggacatcg gcgtgggcaa
     4741 ggacttcatg agcaagaccc ccaaggccat ggccaccaag gacaagatcg acaagtggga
     4801 cctgatcaag ctgaagagct tctgcaccgc caaggagacc accatccgcg tgaaccgcca
     4861 gcccaccacc tgggagaaga tcttcgccac ctacagcagc gacaagggcc tgatcagccg
     4921 catctacaac gagctgaagc agatctacaa gaagaagacc aacaacccca tcaagaagtg
     4981 ggccaaggac atgaaccgcc acttcagcaa ggaggacatc tacgccgcca agaagcatat
     5041 gaagaagtgc agcagcagcc tggccatccg cgagatgcag atcaagacca ccatgcgcta
     5101 ccacctgacc cccgtgcgca tggccatcat caagaagagc ggcaacaacc gctgctggcg
     5161 cggctgcggc gagatcggca ccctgctgca ctgctggtgg gactgcaagc tggtgcagcc
     5221 cctgtggaag agcgtgtggc gcttcctgcg cgacctggag ctggagatcc ccttcgaccc
     5281 cgccatcccc ctgctgggca tctaccccaa cgagtacaag agctgctgct acaaggacac
     5341 ctgcacccgc atgttcatcg ccgccctgtt caccatcgcc aagacctgga accagcccaa
     5401 gtgccccacc atgatcgatt ggatcaagaa gatgtggcac atctacacca tggagtacta
     5461 cgccgccatc aagaacgacg agttcatcag cttcgtgggc acctggatga agctggagac
     5521 catcatcctg agcaagctga gccaggagca gaagaccaag caccgcatct tcagcctgat
     5581 cggcggcaac tgaggcctgt caggccaagc ttccatcgat agacatgata agatacattg
     5641 atgagtttgg acaaaccaca acaagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5701 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     5761 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt
     5821 aaaacctcta caaatgtggt acctaggcga gaccctgtct cacaaaataa agtaagcccg
     5881 gactgagtgc ggaaaggcgg gcctggcggg tctggtctcc ccatgcgggc caccagaggc
     5941 cctgcagcct tcagtcgctt gaaggggtaa tggcgcttcc actcacaaac atggcggaca
     6001 gagcgtgtga acgagatgaa cagcccctca aaaatatggc cgccgaggct ggacggccgt
     6061 gccccagcag caccgcctcc gcgccccacg tgatctctcg ccgggcacag cgctgaccgc
     6121 ggaggtccaa ccggaagaat gtccggattg gacattcgga agagggcccg ccttccctgg
     6181 ggaatctctg cgcacgcgca gaacgcttcg accaatgaaa acacaggaag ccgtccgcgc
     6241 aaccgcgttg cgtcacttct gccgcccctg tttcaaggta tatagccgta gacggaactt
     6301 cgcctttctc tcggccttag cgccattttt ttgggtgagt gttttttggt tcctgcgttg
     6361 ggattccgtg tacaatccat agacatctga cctcggcact tagcatcatc acagcaaact
     6421 aactgtagcc tttctctctt tccctgtaga aacctctgca cctgaggcca ccatggtgag
     6481 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     6541 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6601 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6661 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     6721 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     6781 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6841 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6901 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     6961 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     7021 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     7081 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7141 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcagtggagc
     7201 tactaacttc agcctgctga agcaggctgg tgacgtcgag gagaatcctg gccccatgtc
     7261 tagactggac aagagcaaag tcataaacgg cgctctggaa ttactcaatg gagtcggtat
     7321 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     7381 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     7441 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     7501 cgccaagtca ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     7561 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     7621 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     7681 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     7741 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     7801 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     7861 gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca tgctcccagc
     7921 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     7981 ccttgacatg ctccccgggt ccggcgctac taacttcagc ctgctgaagc aggctggtga
     8041 cgtcgaggag aatcctggtc ccatgattga acaagatgga ttgcacgcag gttctccggc
     8101 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     8161 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     8221 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     8281 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     8341 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     8401 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     8461 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8521 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8581 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8641 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8701 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8761 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8821 catcgccttc tatcgccttc ttgacgagtt cttctgattc gaaggcctgt cgtgaagctt
     8881 ggggatcaat tctctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     8941 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     9001 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     9061 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     9121 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggc ctactagcta
     9181 ctcgggaccc cttaccgaaa catcgccgca ttctgcagag gagtcgagtg tatgtaaact
     9241 tctgacccac tgggaatgtg atgaaagaaa taaaagctga aatgaatcat tctctctact
     9301 attattctga tatttcacat tcttaaaata aagtggtgat cctaactgac ctaagacagg
     9361 gaatttttac taggattaaa tgtcaggaat tgtgaaaaag tgagtttaaa tgtatttggc
     9421 taaggtgtat gtaaacttcc gacttcaact gtatagggat ccgcttcctc gctcactgac
     9481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9901 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9961 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10021 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10081 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10141 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10201 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10261 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10321 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11281 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11341 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga
    11401 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
    11461 ttgttaaaat t
//
