LOCUS       sequence-252265-       11444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generation of Tet-On RPE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA093
FEATURES             Location/Qualifiers
     source          1..11444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    77..95
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    113..131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    149..167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    184..202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     283..307
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5320..5441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5331..5350)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5385..5404
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             6164..6865
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6297..6315)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6565..6584
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6759..6782
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6875..6931
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6932..7549,7550..7555,7556..7591,7592..7594,7595..7630,
                     7631..7633,7634..7669,7670..7675)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             7682..7738
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7733..8533
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7793..7812)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8403..8422
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8588..8605)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8594..8818
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9144..9161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9315..9903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9395..9414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10074..10865,10866..10934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10697..10716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10935..11039)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(11442..11444,1..310)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
ORIGIN
        1 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
       61 acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
      121 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      181 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgat taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg
      301 tcagatcgcc tggagccaat tccaactctt ttgccttata cctactttcc gtaccacttc
      361 ctaccctcga aaggcctctg atgggcaaga agcagaaccg caagaccggc aacagcaaga
      421 cccagagcgc cagccccccc cccaaggagc gcagcagcag ccccgccacc gagcagagct
      481 ggatggagaa cgacttcgac gagctgcgcg aggagggctt ccgccgcagc aactacagcg
      541 agctgcgcga ggacatccag accaagggca aggaggtgga gaacttcgag aagaacctgg
      601 aggagtgcat cacccgcatc accaacaccg agaagtgcct gaaggagctg atggagctga
      661 agaccaaggc ccgcgagctg cgcgaggagt gccgcagcct gcgcagccgc tgcgaccaat
      721 tggaggagcg cgtgagcgcc atggaggacg agatgaacga gatgaagcgc gagggcaagt
      781 tccgcgagaa gcgcatcaag cgcaacgagc agagcctgca ggagatctgg gactacgtga
      841 agcgccccaa cctgcgcctg atcggcgtgc ccgagagcga cgtggagaac ggcaccaagc
      901 tggagaacac cctgcaggac atcatccagg agaacttccc caacctggcc cgccaggcca
      961 acgtgcagat ccaggagatc cagcgcaccc cccagcgcta cagcagccgc cgcgccaccc
     1021 cccgccacat catcgtgcgc ttcaccaagg tggagatgaa ggagaagatg ctgcgcgccg
     1081 cccgcgagaa gggccgcgtg accctgaagg gcaagcccat ccgtctcacc gccgacctga
     1141 gcgccgagac cctgcaggcc cgccgcgagt ggggccccat cttcaacatc ctgaaggaga
     1201 agaacttcca gccccgcatc agctaccccg ccaagctgag cttcatcagc gagggcgaga
     1261 tcaagtactt catcgacaag cagatgctgc gcgacttcgt gaccacccgc cccgccctga
     1321 aggagctgct gaaggaggcc ctgaacatgg agcgcaacaa ccgctaccag cccctgcaga
     1381 accacgccaa gatgtaaaga ccatcgagac taggaagaaa ctgcatcaac taatgagcaa
     1441 aatcaggcgc gcccatcata atgaccggca gcaacagcca catcaccatc ctgaccctga
     1501 acatcaacgg cctgaacagc gccatcaagc gccaccgcct ggccagctgg atcaagagcc
     1561 aggaccccag cgtgtgctgc atccaggaga cccacctgac ctgccgcgac acccaccgcc
     1621 tgaagatcaa gggctggcgc aagatctacc aggccaacgg caagcagaag aaggccggcg
     1681 tggccatcct ggtgagcgac aagaccgact tcaagcccac caagatcaag cgcgacaagg
     1741 agggccacta catcatggtg aagggcagca tccagcagga ggagctgacc atcctgaaca
     1801 tctacgcccc caacaccggt gccccccgct tcatcaagca ggtgctgagc gacctgcagc
     1861 gcgacctgga cagccacacc ctgatcatgg gcgacttcaa cacccccctg agcaccctgg
     1921 accgcagcac ccgccagaag gtgaacaagg acacccagga gctgaacagc gccctgcacc
     1981 aggccgacct gatcgacatc taccgcaccc tgcaccccaa gagcaccgag tacaccttct
     2041 tcagcgcccc ccaccacacc tacagcaaga tcgaccacat cgtgggcagc aaggccctgc
     2101 tgagcaagtg caagcgcacc gagatcatca ccaactacct gagcgaccac agcgccatca
     2161 agctggagct gcgcatcaag aacctgaccc agagccgcag caccacctgg aagctgaaca
     2221 acctgctgct gaacgactac tgggtgcaca acgagatgaa ggccgagatc aagatgttct
     2281 tcgaaaccaa cgagaacaag gacaccacct accagaacct gtgggacgcc ttcaaggccg
     2341 tgtgccgcgg caagttcatc gccctgaacg cctacaagcg caagcaggag cgcagcaaga
     2401 tcgacaccct gaccagccag ctgaaggagc tggagaagca ggagcagacc cacagcaagg
     2461 ccagccgccg ccaggagatc accaagatcc gcgccgagct gaaggagatc gagacccaga
     2521 agaccctgca gaagatcaac gagtcgcgaa gctggttctt cgagcgcatc aacaagatcg
     2581 accgccccct ggcccgcctg atcaagaaga agcgcgagaa gaaccagatc gacaccatca
     2641 agaacgacaa gggcgacatc accaccgacc ccaccgagat ccagaccacc atccgcgagt
     2701 actacaagca cctgtacgcc aacaagctgg agaacctgga ggagatggac accttcctgg
     2761 acacctacac cctgccccgc ctgaaccagg aggaggtgga gagcctgaac cgccccatca
     2821 ccggcagcga gatcgtggcc atcatcaaca gcctgcccac caagaagagc cccggccccg
     2881 acggcttcac cgccgagttc taccagcgct acaaggagga gctggtgccc ttcctgctga
     2941 agctgttcca gagcatcgag aaggagggca tcctgcccaa cagcttctac gaggccagca
     3001 tcatcctgat ccccaagccc ggccgcgaca ccaccaagaa ggagaacttc cgccccatca
     3061 gcctgatgaa catcgacgcc aagatcctga acaagatcct ggccaaccgc atccagcagc
     3121 acatcaagaa gctgatccac cacgaccagg tgggcttcat ccccgggatg cagggctggt
     3181 tcaacatccg caagagcatc aacgtgatcc agcacatcaa ccgcgccaag gacaagaacc
     3241 acatgatcat cagcatcgac gccgagaagg ccttcgacaa gatccagcag cccttcatgc
     3301 tgaagaccct gaacaagctg ggcatcgacg gcacctactt caagatcatc cgcgccatct
     3361 acgacaagcc caccgccaac atcatcctga acggccagaa gctggaggcc ttccccctga
     3421 agaccggcac gcgtcagggc tgccccctga gccccctgct gttcaacatc gtgctggagg
     3481 tgctggcccg cgccatccgc caggagaagg agatcaaggg catccagctg ggcaaggagg
     3541 aggtgaagct gagcctgttc gccgacgaca tgatcgtgta cctggagaac cccatcgtga
     3601 gcgcccagaa cctgctgaag ctgatcagca acttcagcaa ggtgagcggc tacaagatca
     3661 acgtgcagaa gagccaggcc ttcctgtaca ccaacaaccg ccagaccgag agccagatca
     3721 tgggcgagct gcccttcacc atcgctagca agcgcatcaa gtacctgggc atccagctga
     3781 cccgcgacgt gaaggacctg ttcaaggaga actacaagcc cctgctgaag gagatcaagg
     3841 aggagaccaa caagtggaag aacatcccct gcagctgggt gggccgcatc aacatcgtga
     3901 agatggccat cctgcccaag gtgatctacc gcttcaacgc catccccatc aagctgccca
     3961 tgaccttctt caccgagctg gagaagacca ccctgaagtt catctggaac cagaagcgcg
     4021 cccgcatcgc caagagcatc ctgagccaga agaacaaggc cggcggcatc accctgcccg
     4081 acttcaagct gtactacaag gccaccgtga ccaagaccgc ctggtactgg taccagaacc
     4141 gcgatatcga ccagtggaac cgcaccgagc ccagcgagat catgccccac atctacaact
     4201 acctgatctt cgacaagccc gagaagaaca agcagtgggg caaggacagc ctgttcaaca
     4261 agtggtgctg ggagaactgg ctggccatct gccgcaagct gaagctggac cccttcctga
     4321 ccccctacac caagatcaac agccgctgga tcaaggacct gaacgtgaag cccaagacca
     4381 tcaagaccct ggaggagaac ctgggcatca ccatccagga catcggcgtg ggcaaggact
     4441 tcatgagcaa gacccccaag gccatggcca ccaaggacaa gatcgacaag tgggacctga
     4501 tcaagctgaa gagcttctgc accgccaagg agaccaccat ccgcgtgaac cgccagccca
     4561 ccacctggga gaagatcttc gccacctaca gcagcgacaa gggcctgatc agccgcatct
     4621 acaacgagct gaagcagatc tacaagaaga agaccaacaa ccccatcaag aagtgggcca
     4681 aggacatgaa ccgccacttc agcaaggagg acatctacgc cgccaagaag catatgaaga
     4741 agtgcagcag cagcctggcc atccgcgaga tgcagatcaa gaccaccatg cgctaccacc
     4801 tgacccccgt gcgcatggcc atcatcaaga agagcggcaa caaccgctgc tggcgcggct
     4861 gcggcgagat cggcaccctg ctgcactgct ggtgggactg caagctggtg cagcccctgt
     4921 ggaagagcgt gtggcgcttc ctgcgcgacc tggagctgga gatccccttc gaccccgcca
     4981 tccccctgct gggcatctac cccaacgagt acaagagctg ctgctacaag gacacctgca
     5041 cccgcatgtt catcgccgcc ctgttcacca tcgccaagac ctggaaccag cccaagtgcc
     5101 ccaccatgat cgattggatc aagaagatgt ggcacatcta caccatggag tactacgccg
     5161 ccatcaagaa cgacgagttc atcagcttcg tgggcacctg gatgaagctg gagaccatca
     5221 tcctgagcaa gctgagccag gagcagaaga ccaagcaccg catcttcagc ctgatcggcg
     5281 gcaactgatg tcaggccaag cttccatcga tagacatgat aagatacatt gatgagtttg
     5341 gacaaaccac aacaagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     5401 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     5461 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     5521 acaaatgtgg tacctaggcg agaccctgtc tcacaaaata aagtaagccc ggactgagtg
     5581 cggaaaggcg ggcctggcgg gtctggtctc cccatgcggg ccaccagagg ccctgcagcc
     5641 ttcagtcgct tgaaggggta atggcgcttc cactcacaaa catggcggac agagcgtgtg
     5701 aacgagatga acagcccctc aaaaatatgg ccgccgaggc tggacggccg tgccccagca
     5761 gcaccgcctc cgcgccccac gtgatctctc gccgggcaca gcgctgaccg cggaggtcca
     5821 accggaagaa tgtccggatt ggacattcgg aagagggccc gccttccctg gggaatctct
     5881 gcgcacgcgc agaacgcttc gaccaatgaa aacacaggaa gccgtccgcg caaccgcgtt
     5941 gcgtcacttc tgccgcccct gtttcaaggt atatagccgt agacggaact tcgcctttct
     6001 ctcggcctta gcgccatttt tttgggtgag tgttttttgg ttcctgcgtt gggattccgt
     6061 gtacaatcca tagacatctg acctcggcac ttagcatcat cacagcaaac taactgtagc
     6121 ctttctctct ttccctgtag aaacctctgc acctgaggcc accatgttga gcaagggcga
     6181 ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     6241 tgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     6301 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     6361 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     6421 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     6481 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     6541 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagacaatgg gctgggaggc
     6601 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     6661 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     6721 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     6781 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     6841 gtacggcatg gacgagctgt acaagggcag tggagctact aacttcagcc tgctgaagca
     6901 ggctggtgac gtcgaggaga atcctggccc catgtctaga ctggacaaga gcaaagtcat
     6961 aaacggcgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact
     7021 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     7081 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     7141 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct
     7201 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     7261 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     7321 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     7381 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     7441 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     7501 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccggccga
     7561 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     7621 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtccgg
     7681 cgctactaac ttcagcctgc tgaagcaggc tggtgacgtc gaggagaatc ctggtcccat
     7741 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7801 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7861 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7921 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7981 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     8041 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     8101 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8161 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8221 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     8281 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8341 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8401 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8461 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8521 cgagttcttc tgattcgaag gcctgtcgtg aagcttgggg atcaattctc tagagctcgc
     8581 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8641 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8701 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8761 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8821 tctgaggcgg aaagaaccag ctgggcctac tagctactcg ggacccctta ccgaaacatc
     8881 gccgcattct gcagaggagt cgagtgtatg taaacttctg acccactggg aatgtgatga
     8941 aagaaataaa agctgaaatg aatcattctc tctactatta ttctgatatt tcacattctt
     9001 aaaataaagt ggtgatccta actgacctaa gacagggaat ttttactagg attaaatgtc
     9061 aggaattgtg aaaaagtgag tttaaatgta tttggctaag gtgtatgtaa acttccgact
     9121 tcaactgtat agggatccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10201 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11041 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11101 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattgga tccctataca
    11161 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta
    11221 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt
    11281 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat
    11341 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttcg actcctctgc
    11401 agaatgcggc gatgtttcgg taaggggtcc gctatctaga cgag
//
