LOCUS       sequence-252274-       15124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ORFeus expression, WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA007
FEATURES             Location/Qualifiers
     source          1..15124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    190..208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     291..315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      343..352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5278..5301
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5302..5367
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    5577..5711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5854..5876)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6462..8251
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(8557..10482)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10917..10935)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11003..11107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11108..11176,11177..11968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11326..11345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12139..12727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12628..12647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12881..12898
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12979..12998)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13017..13037)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13022..13351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13202..13337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13444..14043
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13444..13463)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13940..13960
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    14273..14407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14483..14502)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14527..14575
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgccgc ggccgcgttt aaacttaatt aagccaccat gggcaagaag
      361 cagaaccgca agaccggcaa cagcaagacc cagagcgcca gccccccccc caaggagcgc
      421 agcagcagcc ccgccaccga gcagagctgg atggagaacg acttcgacga gctgcgcgag
      481 gagggcttcc gccgcagcaa ctacagcgag ctgcgcgagg acatccagac caagggcaag
      541 gaggtggaga acttcgagaa gaacctggag gagtgcatca cccgcatcac caacaccgag
      601 aagtgcctga aggagctgat ggagctgaag accaaggccc gcgagctgcg cgaggagtgc
      661 cgcagcctgc gcagccgctg cgaccaattg gaggagcgcg tgagcgccat ggaggacgag
      721 atgaacgaga tgaagcgcga gggcaagttc cgcgagaagc gcatcaagcg caacgagcag
      781 agcctgcagg agatctggga ctacgtgaag cgccccaacc tgcgcctgat cggcgtgccc
      841 gagagcgacg tggagaacgg caccaagctg gagaacaccc tgcaggacat catccaggag
      901 aacttcccca acctggcccg ccaggccaac gtgcagatcc aggagatcca gcgcaccccc
      961 cagcgctaca gcagccgccg cgccaccccc cgccacatca tcgtgcgctt caccaaggtg
     1021 gagatgaagg agaagatgct gcgcgccgcc cgcgagaagg gccgcgtgac cctgaagggc
     1081 aagcccatcc gtctcaccgc cgacctgagc gccgagaccc tgcaggcccg ccgcgagtgg
     1141 ggccccatct tcaacatcct gaaggagaag aacttccagc cccgcatcag ctaccccgcc
     1201 aagctgagct tcatcagcga gggcgagatc aagtacttca tcgacaagca gatgctgcgc
     1261 gacttcgtga ccacccgccc cgccctgaag gagctgctga aggaggccct gaacatggag
     1321 cgcaacaacc gctaccagcc cctgcagaac cacgccaaga tgtaaagacc atcgagacta
     1381 ggaagaaact gcatcaacta atgagcaaaa tcaggcgcgc ccatcataat gaccggcagc
     1441 aacagccaca tcaccatcct gaccctgaac atcaacggcc tgaacagcgc catcaagcgc
     1501 caccgcctgg ccagctggat caagagccag gaccccagcg tgtgctgcat ccaggagacc
     1561 cacctgacct gccgcgacac ccaccgcctg aagatcaagg gctggcgcaa gatctaccag
     1621 gccaacggca agcagaagaa ggccggcgtg gccatcctgg tgagcgacaa gaccgacttc
     1681 aagcccacca agatcaagcg cgacaaggag ggccactaca tcatggtgaa gggcagcatc
     1741 cagcaggagg agctgaccat cctgaacatc tacgccccca acaccggtgc cccccgcttc
     1801 atcaagcagg tgctgagcga cctgcagcgc gacctggaca gccacaccct gatcatgggc
     1861 gacttcaaca cccccctgag caccctggac cgcagcaccc gccagaaggt gaacaaggac
     1921 acccaggagc tgaacagcgc cctgcaccag gccgacctga tcgacatcta ccgcaccctg
     1981 caccccaaga gcaccgagta caccttcttc agcgcccccc accacaccta cagcaagatc
     2041 gaccacatcg tgggcagcaa ggccctgctg agcaagtgca agcgcaccga gatcatcacc
     2101 aactacctga gcgaccacag cgccatcaag ctggagctgc gcatcaagaa cctgacccag
     2161 agccgcagca ccacctggaa gctgaacaac ctgctgctga acgactactg ggtgcacaac
     2221 gagatgaagg ccgagatcaa gatgttcttc gaaaccaacg agaacaagga caccacctac
     2281 cagaacctgt gggacgcctt caaggccgtg tgccgcggca agttcatcgc cctgaacgcc
     2341 tacaagcgca agcaggagcg cagcaagatc gacaccctga ccagccagct gaaggagctg
     2401 gagaagcagg agcagaccca cagcaaggcc agccgccgcc aggagatcac caagatccgc
     2461 gccgagctga aggagatcga gacccagaag accctgcaga agatcaacga gtcgcgaagc
     2521 tggttcttcg agcgcatcaa caagatcgac cgccccctgg cccgcctgat caagaagaag
     2581 cgcgagaaga accagatcga caccatcaag aacgacaagg gcgacatcac caccgacccc
     2641 accgagatcc agaccaccat ccgcgagtac tacaagcacc tgtacgccaa caagctggag
     2701 aacctggagg agatggacac cttcctggac acctacaccc tgccccgcct gaaccaggag
     2761 gaggtggaga gcctgaaccg ccccatcacc ggcagcgaga tcgtggccat catcaacagc
     2821 ctgcccacca agaagagccc cggccccgac ggcttcaccg ccgagttcta ccagcgctac
     2881 aaggaggagc tggtgccctt cctgctgaag ctgttccaga gcatcgagaa ggagggcatc
     2941 ctgcccaaca gcttctacga ggccagcatc atcctgatcc ccaagcccgg ccgcgacacc
     3001 accaagaagg agaacttccg ccccatcagc ctgatgaaca tcgacgccaa gatcctgaac
     3061 aagatcctgg ccaaccgcat ccagcagcac atcaagaagc tgatccacca cgaccaggtg
     3121 ggcttcatcc ccgggatgca gggctggttc aacatccgca agagcatcaa cgtgatccag
     3181 cacatcaacc gcgccaagga caagaaccac atgatcatca gcatcgacgc cgagaaggcc
     3241 ttcgacaaga tccagcagcc cttcatgctg aagaccctga acaagctggg catcgacggc
     3301 acctacttca agatcatccg cgccatctac gacaagccca ccgccaacat catcctgaac
     3361 ggccagaagc tggaggcctt ccccctgaag accggcacgc gtcagggctg ccccctgagc
     3421 cccctgctgt tcaacatcgt gctggaggtg ctggcccgcg ccatccgcca ggagaaggag
     3481 atcaagggca tccagctggg caaggaggag gtgaagctga gcctgttcgc cgacgacatg
     3541 atcgtgtacc tggagaaccc catcgtgagc gcccagaacc tgctgaagct gatcagcaac
     3601 ttcagcaagg tgagcggcta caagatcaac gtgcagaaga gccaggcctt cctgtacacc
     3661 aacaaccgcc agaccgagag ccagatcatg ggcgagctgc ccttcaccat cgctagcaag
     3721 cgcatcaagt acctgggcat ccagctgacc cgcgacgtga aggacctgtt caaggagaac
     3781 tacaagcccc tgctgaagga gatcaaggag gagaccaaca agtggaagaa catcccctgc
     3841 agctgggtgg gccgcatcaa catcgtgaag atggccatcc tgcccaaggt gatctaccgc
     3901 ttcaacgcca tccccatcaa gctgcccatg accttcttca ccgagctgga gaagaccacc
     3961 ctgaagttca tctggaacca gaagcgcgcc cgcatcgcca agagcatcct gagccagaag
     4021 aacaaggccg gcggcatcac cctgcccgac ttcaagctgt actacaaggc caccgtgacc
     4081 aagaccgcct ggtactggta ccagaaccgc gatatcgacc agtggaaccg caccgagccc
     4141 agcgagatca tgccccacat ctacaactac ctgatcttcg acaagcccga gaagaacaag
     4201 cagtggggca aggacagcct gttcaacaag tggtgctggg agaactggct ggccatctgc
     4261 cgcaagctga agctggaccc cttcctgacc ccctacacca agatcaacag ccgctggatc
     4321 aaggacctga acgtgaagcc caagaccatc aagaccctgg aggagaacct gggcatcacc
     4381 atccaggaca tcggcgtggg caaggacttc atgagcaaga cccccaaggc catggccacc
     4441 aaggacaaga tcgacaagtg ggacctgatc aagctgaaga gcttctgcac cgccaaggag
     4501 accaccatcc gcgtgaaccg ccagcccacc acctgggaga agatcttcgc cacctacagc
     4561 agcgacaagg gcctgatcag ccgcatctac aacgagctga agcagatcta caagaagaag
     4621 accaacaacc ccatcaagaa gtgggccaag gacatgaacc gccacttcag caaggaggac
     4681 atctacgccg ccaagaagca tatgaagaag tgcagcagca gcctggccat ccgcgagatg
     4741 cagatcaaga ccaccatgcg ctaccacctg acccccgtgc gcatggccat catcaagaag
     4801 agcggcaaca accgctgctg gcgcggctgc ggcgagatcg gcaccctgct gcactgctgg
     4861 tgggactgca agctggtgca gcccctgtgg aagagcgtgt ggcgcttcct gcgcgacctg
     4921 gagctggaga tccccttcga ccccgccatc cccctgctgg gcatctaccc caacgagtac
     4981 aagagctgct gctacaagga cacctgcacc cgcatgttca tcgccgccct gttcaccatc
     5041 gccaagacct ggaaccagcc caagtgcccc accatgatcg attggatcaa gaagatgtgg
     5101 cacatctaca ccatggagta ctacgccgcc atcaagaacg acgagttcat cagcttcgtg
     5161 ggcacctgga tgaagctgga gaccatcatc ctgagcaagc tgagccagga gcagaagacc
     5221 aagcaccgca tcttcagcct gatcggcggc aacggcggcg gcagcggcgg cggcagcctg
     5281 gaagttctgt tccaggggcc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5341 atcgattaca aggatgacga tgacaagtag acaatgagat cacatggaca caggaagggg
     5401 aatatcacac tctggggact gtggtggggt cgggggaggg gggagggata gcattgggag
     5461 atatacctaa tgctagatga cacattagtg ggtgcagcgc accagcatgg cacatgtata
     5521 catatgtaac taacctgcac aatgtgcaca tgtaccctaa aacttagagt ataatggatc
     5581 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     5641 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     5701 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     5761 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg
     5821 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     5881 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     5941 gggtgttggc gggtgtcggg gcgcagccat gaccggtcga ctctagagcc ccgccgccgg
     6001 acgaactaaa cctgactacg gcatctctgc cccttcttcg ctggtacgag gagcgctttt
     6061 gttttgtatt cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     6121 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     6181 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     6241 cagactttgc atgctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     6301 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     6361 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     6421 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     6481 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     6541 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     6601 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     6661 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     6721 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     6781 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     6841 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     6901 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     6961 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     7021 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     7081 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     7141 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     7201 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     7261 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     7321 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     7381 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     7441 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     7501 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     7561 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     7621 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     7681 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     7741 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     7801 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     7861 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     7921 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     7981 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     8041 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     8101 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     8161 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     8221 gcgtgaattt tcgctgcttg tccttttcct gcatgctggt tgctcccatt cttaggtgaa
     8281 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
     8341 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
     8401 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
     8461 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
     8521 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
     8581 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
     8641 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
     8701 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
     8761 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
     8821 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
     8881 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
     8941 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
     9001 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
     9061 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
     9121 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
     9181 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
     9241 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
     9301 tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc
     9361 acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc
     9421 ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc
     9481 actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc
     9541 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
     9601 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     9661 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     9721 tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc
     9781 ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     9841 tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     9901 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     9961 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
    10021 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
    10081 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
    10141 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc
    10201 tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt
    10261 ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc
    10321 tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc
    10381 tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc
    10441 taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga
    10501 tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc
    10561 ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg
    10621 aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa
    10681 actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct
    10741 cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt
    10801 caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg
    10861 aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
    10921 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
    10981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    11041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    11101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    11161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    11221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    11281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    11341 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    11401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    11461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    11521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    11581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    11641 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    11701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    11761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    11821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    11881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    11941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    12001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    12061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    12121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    12181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    12241 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
    12301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    12361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    12421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    12481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    12541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    12601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    12661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    12721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    12781 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    12841 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    12901 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
    12961 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agctgtggaa
    13021 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    13081 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    13141 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    13201 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    13261 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    13321 aggctttttt ggaggcctag gcttttgcaa aaagcttgca tgcctgcagg tcggccgcca
    13381 cgaccggtgc cgccaccatc ccctgaccca cgcccctgac ccctcacaag gagacgacct
    13441 tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
    13501 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
    13561 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
    13621 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
    13681 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
    13741 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
    13801 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
    13861 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
    13921 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
    13981 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
    14041 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatggctcc
    14101 gaccgaagcc gacccgggcg gccccgccga ccccgcaccc gcccccgagg cccaccgact
    14161 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    14221 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    14281 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    14341 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    14401 ctggatcact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
    14461 aggggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    14521 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
    14581 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    14641 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    14701 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
    14761 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
    14821 cgtggggtct ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg
    14881 gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc
    14941 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
    15001 tgtttgacag cttatcatcg cagatccggg caacgttgtt gcattgctgc aggcgcagaa
    15061 ctggtaggta tggaagatct ctagaagctg ggtaccagct gctagcaagc ttgctagcgg
    15121 ccgg
//
