LOCUS       sequence-252288-        1803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lck FRET Biosensor. To visualize Lck kinase activity in mammalian 
            cells by FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Wan R, Wu J, Ouyang M, Lei L, Wei J, Peng Q, Harrison R, Wu Y, Cheng
            B, Li K, Zhu C, Tang L, Wang Y, Lu S
  TITLE     Biophysical basis underlying dynamic Lck activation visualized by 
            ZapLck FRET biosensor.
  JOURNAL   Sci Adv. 2019 Jun 19;5(6):eaau2001. doi: 10.1126/sciadv.aau2001. 
            eCollection 2019 Jun.
  PUBMED    31223643
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-06-19"; volume: "5(6):eaau2001. doi"; pages: " 
            10.1126/sciadv.aau2001. eCollection 2019 Jun"
COMMENT     Sequence Label: ZapLck biosensor
FEATURES             Location/Qualifiers
     source          1..1803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7..9,10..12,13..525)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(52..73)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(313..332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     660..681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1090..1800
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
ORIGIN
        1 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaggttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgtacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag
      481 aagaacggca tcaaggccca cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc cgcatgcatt ggtattttgg gaagatcact
      721 cgtcgggagt ccgagcggct gctgctcaac cccgaaaacc cccggggaac cttcttggtc
      781 cgggagagcg agacgacaaa aggtgcctat gccctctccg tttctgactt tgacaacgcc
      841 aaggggctca atgtgaagca ctacaagatc cgcaagctgg acagcggcgg cttctacatc
      901 acctcacgca cacagttcag cagcctgcag cagctggtgg cctactactc caaacatgct
      961 gatggcttgt gccaccgcct gactaacgtc tgtgggtcta catctggatc tgggaagccg
     1021 ggttctggtg agggttctat ggacacgagc gtgttcgaga gcccctacag cgacccagag
     1081 gaggagctct ctaaaggtga agaattattc actggtgttg tcccaatttt ggttgaatta
     1141 gatggtgatg ttaatggtca caaattttct gtctccggtg aaggtgaagg tgatgctacg
     1201 tacggtaaat tgaccttaaa attactctgt actactggta aattgccagt tccatggcca
     1261 accttagtca ctactttagg ttatggtgtt caatgttttg ctagataccc agatcatatg
     1321 aaacaacatg actttttcaa gtctgccatg ccagaaggtt atgttcaaga aagaactatt
     1381 tttttcaaag atgacggtaa ctacaagacc agagctgaag tcaagtttga aggtgatacc
     1441 ttagttaata gaatcgaatt aaaaggtatt gattttaaag aagatggtaa cattttaggt
     1501 cacaaattgg aatacaacta taactctcac aatgtttaca tcactgctga caaacaaaag
     1561 aatggtatca aagctaactt caaaattaga cacaacattg aagatggtgg tgttcaatta
     1621 gctgaccatt atcaacaaaa tactccaatt ggtgatggtc cagtcttgtt accagacaac
     1681 cattacttat cctatcaatc tgccttattc aaagatccaa acgaaaagag agaccacatg
     1741 gtcttgttag aatttttgac tgctgctggt attaccgagg gtatgaatga attgtacaaa
     1801 taa
//
