LOCUS       sequence-25229-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Hageman J, Kampinga HH
  TITLE     Computational analysis of the human HSPH/HSPA/DNAJ family and 
            cloning of a human HSPH/HSPA/DNAJ expression library.
  JOURNAL   Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 
            10.1007/s12192-008-0060-2. Epub 2008 Aug 7.
  PUBMED    18686016
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12192-008-0060-2"; journalName: "Cell Stress Chaperones"; 
            date: "2009-01"; volume: "14"; issue: "1"; pages: "1-21"
COMMENT     Sequence Label: pcDNA5/FRT/TO HIS
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(194..211)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    200..424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(557..576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
       61 acttaagctt ctgcagacca tgcatcatca ccatcaccat ggatccacta gtccagtgtg
      121 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
      181 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      241 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      301 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
      361 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
      421 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
      481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      601 tttccccgtc aagctctaaa tcgnnnnnnc cntttagggt tccgatttag tgctttacgg
      661 cacctcgacc ccaaaaactt gattnnnnnn ntggttcacg tacctagaag ttcctattcc
      721 naagttccta ttctctanaa agtataggaa nttcct
//
