LOCUS       sequence-252360-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pHUBC driving expression of mAzamiGreen with an mScarlet 
            normalization in a PiggyBac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0511
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        540..743
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     694..714
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      765..774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             771..1799
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             771..1799
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    1817..2024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2231..2293
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(2344..2362)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3125..3144
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3209..3228
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4142..4161
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4142..4160
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4190..4224
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        4752..5963
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5800..5819
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5973..5993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6009..6680
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             6009..6680
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     polyA_signal    6707..6931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        7179..7482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          7755..8771
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8695..8717
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8779..8798
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             8832..9209
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(10083..10102)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10148..10203
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10202..10221)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg cttgatgtgg
       61 acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa ccgggctctg
      121 tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca ggaaggaaag
      181 gttgcatgct ggacacacta accttacagc ggtgggctct atggggtacc tacgtaggta
      241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      721 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatagccacc atgggtaaaa
      781 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt
      841 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      901 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      961 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     1021 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     1081 tgcctgaaac cgaactgccc gctgttctgc aaccggtcgc ggaggccatg gatgcgatcg
     1141 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     1201 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     1261 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     1321 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     1381 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     1441 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1501 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     1561 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1621 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1681 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1741 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataac
     1801 tcgagcgacg cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2041 agtatcgata ctagttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt
     2101 tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag
     2161 tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg atatacagac
     2221 cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat
     2281 tatctttcta gggttaatct agtatacgcg tatgcggccg cttaattaat ccagcttttg
     2341 ttccctttag tgagggttaa ttccgagcgg ccgcgtgtta caaccaatta accaattctg
     2401 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2461 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2521 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2581 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2641 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
     2701 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     2761 gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     2821 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     2881 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     2941 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3001 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     3061 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     3121 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     3181 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     3241 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3301 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     3361 atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgccaa
     4141 ttaatacgac tcactatagg gcgaattggg gcgcgccatt ctagattaac cctagaaaga
     4201 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     4261 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     4321 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     4381 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     4441 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa
     4501 ctagaatgct agcatgggcc cagatctcca tgggccctga attcgcatct agactgaact
     4561 ggccgataat tgcagacgag gagcatcgcc cttccccggc cctcaggtaa gaggaccaaa
     4621 taccgtagcc gtttccaatt tcagtccttt agcgccacct ggtgctaact actctatcac
     4681 gcttttatcc aataactacc tttgtaaatt tcctttcaaa agttctggcc gggcgcggtg
     4741 gcaacggatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     4801 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     4861 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     4921 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     4981 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     5041 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     5101 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     5161 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     5221 ggagacaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     5281 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     5341 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     5401 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     5461 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     5521 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     5581 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     5641 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     5701 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     5761 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     5821 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     5881 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     5941 cgtttttggc ttttttgtta gacgccacta tgcccaagaa gaagaggaag gtggaggacg
     6001 ccggttctgt gagcgtgatc aagcccgaga tgaagatcaa gctgtgcatg aggggcaccg
     6061 tgaacggcca caacttcgtg atcgagggcg agggcaaggg caacccctac gagggcaccc
     6121 agatcctgga cctgaacgtg accgagggcg cccccctgcc cttcgcctac gacatcctga
     6181 ccaccgtgtt ccagtacggc aacagggcct tcaccaagta ccccgccgac atccaggact
     6241 acttcaagca gaccttcccc gagggctacc actgggagag gagcatgacc tacgaggacc
     6301 agggcatctg caccgccacc agcaacatca gcatgagggg cgactgcttc ttctacgaca
     6361 tcaggttcga cggcaccaac ttccccccca acggccccgt gatgcagaag aagaccctga
     6421 agtgggagcc cagcaccgag aagatgtacg tggaggacgg cgtgctgaag ggcgacgtga
     6481 acatgaggct gctgctggag ggcggcggcc actacaggtg cgacttcaag accacctaca
     6541 aggccaagaa ggaggtgagg ctgcccgacg cccacaagat cgaccacagg atcgagatcc
     6601 tgaagcacga caaggactac aacaaggtga agctgtacga gaacgccgtg gccaggtact
     6661 ccatgctgcc cagccaggcc ggatcctaac tcgagcgacg cctcgactgt gccttctagt
     6721 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6781 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6841 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6901 aggcatgctg gggatgcggt gggctctatg ggctggttct gtatgggcac agacaaccta
     6961 actaatttcc tggccctcaa ggtgacttgc ttgaacttgc caccttcgcc actagggggc
     7021 agcattggtt tacacagggt gaacaaacgt tcaattctaa actaatattc tttggtggga
     7081 aagtgtgttc attttggttt gttttgaaga agtcacttca tacctttgaa ctggggttaa
     7141 gctggttaac tcccaaaaac gccatatatg gagttccgcg ttacataact tacggtaaat
     7201 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     7261 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     7321 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     7381 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     7441 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     7501 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     7561 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggcgcgcgc caggcggggc
     7621 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     7681 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     7741 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     7801 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     7861 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     7921 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     7981 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     8041 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     8101 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     8161 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     8221 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     8281 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     8341 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     8401 cccggagtgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     8461 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     8521 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     8581 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     8641 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     8701 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     8761 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     8821 aattcgccac tatgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga
     8881 aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga
     8941 gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt
     9001 ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg
     9061 aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga
     9121 cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc gagggtacta
     9181 aggccatcac caagtacacc agcgctaagg gttctgtatc aaaaggcgag gcggtcatta
     9241 aagagttcat gaggtttaaa gtccatatgg aaggaagcat gaatggtcac gagtttgaga
     9301 tagagggaga gggtgaggga aggccgtacg aagggacgca aactgcgaag ttgaaggtaa
     9361 cgaaaggcgg ccctctgcca ttctcttggg acatcctttc accgcaattt atgtacggct
     9421 ctagggcctt cacaaaacac cccgccgata ttccggatta ctataaacaa agttttcctg
     9481 agggcttcaa gtgggaaaga gtaatgaact tcgaggatgg gggagctgtg acggttactc
     9541 aagacactag cctggaggac ggtactctta tatacaaggt aaaactaaga gggacgaatt
     9601 ttccgccaga cggccccgtg atgcaaaaaa aaactatggg gtgggaagca tccaccgaga
     9661 ggctgtaccc cgaagacggg gtcctgaagg gtgatattaa aatggcatta agattaaaag
     9721 atggcgggag atacttagct gacttcaaga ctacatataa agccaaaaaa cctgtacaga
     9781 tgcctggagc ttacaatgtg gatcgtaagc tggatattac atcccacaac gaagattata
     9841 ccgtggtgga gcagtacgag cgttcagagg ggcgtcactc caccgggggt atggatgaat
     9901 tatacaagag cggtcttaga agcagggcgc aagcgtcaaa ttcagcggta gatggaacag
     9961 caggtccagg tagtactggc agcagaggat cctaactcga gattcactcc tcaggtgcag
    10021 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
    10081 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
    10141 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
    10201 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
    10261 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
    10321 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
    10381 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
    10441 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
    10501 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatg
    10561 ctgagca
//
