LOCUS       sequence-252363-        5205 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa27 of Flp recombinase fused to nMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2665_pCAG-PV1-FlpO-N27-L1-nMag-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5205
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1250..1270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1280..1504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1744..1763)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1809..1864
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1863..1882)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2225..2241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2225..2241)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2238..2260)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2249..2265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2273..2279,2280..2297,2298..2303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2318..2339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2424..2558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2578..2712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2615..2634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2669..2688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2780..2797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2951..3539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3031..3050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3710..4501,4502..4570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4333..4352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4571..4675)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4706..5085
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga gccagttcga catcctgtgc aagacccccc ccaaggtgct
      721 ggtgcggcag ttcgtggaga gattcgagag gcccagctcc ggagggtctg gctccggatc
      781 aagtggtggc agcggtaccc atactcttta tgcccccggt ggatatgaca ttatgggata
      841 tctggaccag atcggcaacc ggccaaaccc gcaggtggaa ctgggccccg tggatacatc
      901 ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt acgcgagtga
      961 agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga attgccggtt
     1021 tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg tggatagtaa
     1081 cacaatcaat actatgcgca aggcaatcga caggaatgcc gaggtgcagg ttgaagtagt
     1141 caattttaaa aagaatggac agcgatttgt taatttcctg actatgatac ctgttaggga
     1201 cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaac ccaagaaaaa
     1261 gcggaaggtg tgagaattcc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     1321 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     1381 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     1441 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     1501 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     1561 agcgtgtacc gctgggagtt cgtagacgga aacaaacgca gaatccaagc gcggccgccc
     1621 aggatacata gattaccaca actccgagcc cttccaccgc tagcgcactc ctcaggtgca
     1681 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     1741 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     1801 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     1861 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     1921 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     1981 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     2041 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     2101 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     2161 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     2221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     2401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccagttccg cccattctcc
     2461 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     2521 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     2581 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2641 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2701 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2761 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2821 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2881 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2941 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3001 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3061 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3121 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3181 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3241 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3301 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3361 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3421 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3481 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3541 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4681 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca
     4741 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4801 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4861 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4921 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4981 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     5041 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     5101 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     5161 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggg
//
