LOCUS       sequence-252394-       12223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MAN2B1 E149V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MAN2B1-E149V
FEATURES             Location/Qualifiers
     source          1..12223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     LTR             1052..1309
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1356..1481
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1978..2211
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2396..2440
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2733..2850
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2898..4076
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3129..4067
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4024..4044
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4096..4120
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4117..4126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7160..7184)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7237..7278
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7299..7866
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7316..7868
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7460..7477)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7687..7706
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7880..7882,7883..7885,7886..8599)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7880..7882,7883..7885,7886..8596)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7925..7946)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8186..8205)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8533..8554
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8613..9201
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8666..8686)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9084..9095)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9276..9509
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9714..9818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9819..9887,9888..10679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10037..10056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10850..11438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11339..11358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11592..11609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11849..11870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11885..11890,11891..11908,11909..11915)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11923..11939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11928..11950
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11947..11963
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11947..11963
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12013..12032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
      661 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
      721 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      781 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      841 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatcgtg agcctgcatg
      901 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      961 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     1021 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     1081 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     1141 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1201 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1261 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1321 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1381 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1441 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1501 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1561 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1621 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1681 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1741 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1801 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1861 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1921 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1981 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     2041 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     2101 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     2161 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     2221 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     2281 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2341 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2401 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2461 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2521 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2581 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2641 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2701 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2761 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2821 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2881 gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2941 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     3001 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     3061 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     3121 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     3181 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     3241 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     3301 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3361 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3421 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     3481 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3541 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3601 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     3661 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3721 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3781 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3841 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3901 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3961 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     4021 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg
     4081 gccctgcaga tatcaacaag tttgtacaaa aaagcagcca ccatgggcgc ctacgcgcgg
     4141 gcttcggggg tctgcgctcg cggctgcctg gactcagcag gcccctggac catgtcccgc
     4201 gccctgcggc caccgctccc gcctctctgc tttttccttt tgttgctggc ggctgccggt
     4261 gctcgggccg ggggatacga gacatgcccc acagtgcagc cgaacatgct gaacgtgcac
     4321 ctgctgcctc acacacatga tgacgtgggc tggctcaaaa ccgtggacca gtacttttat
     4381 ggaatcaaga atgacatcca gcacgccggt gtgcagtaca tcctggactc ggtcatctct
     4441 gccttgctgg cagatcccac ccgtcgcttc atttacgtgg agattgcctt cttctcccgt
     4501 tggtggcacc agcagacaaa tgccacacag gaagtcgtgc gagaccttgt gcgccagggg
     4561 cgcctggtgt tcgccaatgg tggctgggtg atgaacgatg aggcagccac ccactacggt
     4621 gccatcgtgg accagatgac acttgggctg cgctttctgg aggacacatt tggcaatgat
     4681 gggcgacccc gtgtggcctg gcacattgac cccttcggcc actctcggga gcaggcctcg
     4741 ctgtttgcgc agatgggctt cgacggcttc ttctttgggc gccttgatta tcaagataag
     4801 tgggtacgga tgcagaagct ggagatggag caggtgtggc gggccagcac cagcctgaag
     4861 cccccgaccg cggacctctt cactggtgtg cttcccaatg gttacaaccc gccaaggaat
     4921 ctgtgctggg atgtgctgtg tgtcgatcag ccgctggtgg aggaccctcg cagccccgag
     4981 tacaacgcca aggagctggt cgattacttc ctaaatgtgg ccactgccca gggccggtat
     5041 taccgcacca accacactgt gatgaccatg ggctcggact tccaatatga gaatgccaac
     5101 atgtggttca agaaccttga caagctcatc cggctggtaa atgcgcagca ggcaaaagga
     5161 agcagtgtcc atgttctcta ctccaccccc gcttgttacc tctgggagct gaacaaggcc
     5221 aacctcacct ggtcagtgaa acatgacgac ttcttccctt acgcggatgg cccccaccag
     5281 ttctggaccg gttacttttc cagtcggccg gccctcaaac gctacgagcg cctcagctac
     5341 aacttcctgc aggtgtgcaa ccagctggag gcgctggtgg gcctggcggc caacgtggga
     5401 ccctatggct ccggagacag tgcacccctc aatgaggcga tggctgtgct ccagcatcac
     5461 gacgccgtca gcggcacctc ccgccagcac gtggccaacg actacgcgcg ccagcttgcg
     5521 gcaggctggg ggccttgcga ggttcttctg agcaacgcgc tggcgcggct cagaggcttc
     5581 aaagatcact tcaccttttg ccaacagcta aacatcagca tctgcccgct cagccagacg
     5641 gcggcgcgct tccaggtcat cgtttataat cccctggggc ggaaggtgaa ttggatggta
     5701 cggctgccgg tcagcgaagg cgttttcgtt gtgaaggacc ccaatggcag gacagtgccc
     5761 agcgatgtgg taatatttcc cagctcagac agccaggcgc accctccgga gctgctgttc
     5821 tcagcctcac tgcccgccct gggcttcagc acctattcag tagcccaggt gcctcgctgg
     5881 aagccccagg cccgcgcacc acagcccatc cccagaagat cctggtcccc tgctttaacc
     5941 atcgaaaatg agcacatccg ggcaacgttt gatcctgaca cagggctgtt gatggagatt
     6001 atgaacatga atcagcaact cctgctgcct gttcgccaga ccttcttctg gtacaacgcc
     6061 agtataggtg acaacgaaag tgaccaggcc tcaggtgcct acatcttcag acccaaccaa
     6121 cagaaaccgc tgcctgtgag ccgctgggct cagatccacc tggtgaagac acccttggtg
     6181 caggaggtgc accagaactt ctcagcttgg tgttcccagg tggttcgcct gtacccagga
     6241 cagcggcacc tggagctaga gtggtcggtg gggccgatac ctgtgggcga cacctggggg
     6301 aaggaggtca tcagccgttt tgacacaccg ctggagacaa agggacgctt ctacacagac
     6361 agcaatggcc gggagatcct ggagaggagg cgggattatc gacccacctg gaaactgaac
     6421 cagacggagc ccgtggcagg aaactactat ccagtcaaca cccggattta catcacggat
     6481 ggaaacatgc agctgactgt gctgactgac cgctcccagg ggggcagcag cctgagagat
     6541 ggctcgctgg agctcatggt gcaccgaagg ctgctgaagg acgatggacg cggagtatcg
     6601 gagccactaa tggagaacgg gtcgggggcg tgggtgcgag ggcgccacct ggtgctgctg
     6661 gacacagccc aggctgcagc cgccggacac cggctcctgg cggagcagga ggtcctggcc
     6721 cctcaggtgg tgctggcccc gggtggcggc gccgcctaca atctcggggc tcctccgcgc
     6781 acgcagttct cagggctgcg cagggacctg ccgccctcgg tgcacctgct cacgctggcc
     6841 agctggggcc ccgaaatggt gctgctgcgc ttggagcacc agtttgccgt aggagaggat
     6901 tccggacgta acctgagcgc ccccgttacc ttgaacttga gggacctgtt ctccaccttc
     6961 accatcaccc gcctgcagga gaccacgctg gtggccaacc agctccgcga ggcagcctcc
     7021 aggctcaagt ggacaacaaa cacaggcccc acaccccacc aaactccgta ccagctggac
     7081 ccggccaaca tcacgctgga acccatggaa atccgcactt tcctggcctc agttcaatgg
     7141 aaggaggtgg atggttagaa cccagctttc ttgtacaaag tggttgatat ccagcacagt
     7201 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     7261 ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc ccctaacgtt
     7321 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     7381 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     7441 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     7501 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     7561 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     7621 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     7681 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     7741 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     7801 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     7861 tgataatatg gccacacata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7921 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     7981 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     8041 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     8101 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     8161 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     8221 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     8281 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     8341 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     8401 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     8461 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     8521 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     8581 ggacgagctg tacaagtaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg
     8641 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8701 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8761 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8821 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8881 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8941 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     9001 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     9061 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     9121 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     9181 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt
     9241 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9301 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     9361 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9421 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9481 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     9541 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag
     9601 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     9661 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     9721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
    10081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    10441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    10501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    10561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    10681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    11581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    11641 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt
    11701 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    11761 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
    11821 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    11881 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    11941 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
    12001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    12061 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    12121 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    12181 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
