LOCUS       sequence-252415-        9522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet 
            normalization in a PiggyBac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0519
FEATURES             Location/Qualifiers
     source          1..9522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        540..743
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      765..774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             771..1799
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             771..1799
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    1817..2024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2231..2293
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(2344..2362)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3125..3144
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3209..3228
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4142..4161
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4142..4160
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4190..4224
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        4781..4880
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     CDS             4928..4948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4964..5635
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             4964..5635
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     polyA_signal    5662..5886
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6134..6437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          6710..7726
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7650..7672
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7734..7753
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7787..8164
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(9038..9057)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9103..9158
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9157..9176)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg cttgatgtgg
       61 acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa ccgggctctg
      121 tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca ggaaggaaag
      181 gttgcatgct ggacacacta accttacagc ggtgggctct atggggtacc tacgtaggta
      241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      721 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatagccacc atgggtaaaa
      781 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt
      841 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      901 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      961 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     1021 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     1081 tgcctgaaac cgaactgccc gctgttctgc aaccggtcgc ggaggccatg gatgcgatcg
     1141 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     1201 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     1261 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     1321 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     1381 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     1441 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1501 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     1561 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1621 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1681 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1741 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataac
     1801 tcgagcgacg cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2041 agtatcgata ctagttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt
     2101 tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag
     2161 tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg atatacagac
     2221 cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat
     2281 tatctttcta gggttaatct agtatacgcg tatgcggccg cttaattaat ccagcttttg
     2341 ttccctttag tgagggttaa ttccgagcgg ccgcgtgtta caaccaatta accaattctg
     2401 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2461 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2521 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2581 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2641 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
     2701 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     2761 gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     2821 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     2881 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     2941 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3001 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     3061 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     3121 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     3181 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     3241 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3301 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     3361 atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgccaa
     4141 ttaatacgac tcactatagg gcgaattggg gcgcgccatt ctagattaac cctagaaaga
     4201 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     4261 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     4321 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     4381 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     4441 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa
     4501 ctagaatgct agcatgggcc cagatctcca tgggccctga attcgcatct agactgaact
     4561 ggccgataat tgcagacgag gagcatcgcc cttccccggc cctcaggtaa gaggaccaaa
     4621 taccgtagcc gtttccaatt tcagtccttt agcgccacct ggtgctaact actctatcac
     4681 gcttttatcc aataactacc tttgtaaatt tcctttcaaa agttctggcc gggcgcggtg
     4741 gcaacgtagt tattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     4801 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     4861 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggtcgc
     4921 cactatgccc aagaagaaga ggaaggtgga ggacgccggt tctgtgagcg tgatcaagcc
     4981 cgagatgaag atcaagctgt gcatgagggg caccgtgaac ggccacaact tcgtgatcga
     5041 gggcgagggc aagggcaacc cctacgaggg cacccagatc ctggacctga acgtgaccga
     5101 gggcgccccc ctgcccttcg cctacgacat cctgaccacc gtgttccagt acggcaacag
     5161 ggccttcacc aagtaccccg ccgacatcca ggactacttc aagcagacct tccccgaggg
     5221 ctaccactgg gagaggagca tgacctacga ggaccagggc atctgcaccg ccaccagcaa
     5281 catcagcatg aggggcgact gcttcttcta cgacatcagg ttcgacggca ccaacttccc
     5341 ccccaacggc cccgtgatgc agaagaagac cctgaagtgg gagcccagca ccgagaagat
     5401 gtacgtggag gacggcgtgc tgaagggcga cgtgaacatg aggctgctgc tggagggcgg
     5461 cggccactac aggtgcgact tcaagaccac ctacaaggcc aagaaggagg tgaggctgcc
     5521 cgacgcccac aagatcgacc acaggatcga gatcctgaag cacgacaagg actacaacaa
     5581 ggtgaagctg tacgagaacg ccgtggccag gtactccatg ctgcccagcc aggccggatc
     5641 ctaactcgag cgacgcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5701 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5761 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5821 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5881 ctatgggctg gttctgtatg ggcacagaca acctaactaa tttcctggcc ctcaaggtga
     5941 cttgcttgaa cttgccacct tcgccactag ggggcagcat tggtttacac agggtgaaca
     6001 aacgttcaat tctaaactaa tattctttgg tgggaaagtg tgttcatttt ggtttgtttt
     6061 gaagaagtca cttcatacct ttgaactggg gttaagctgg ttaactccca aaaacgccat
     6121 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     6181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     6241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     6301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     6361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6421 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     6481 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     6541 gggcgggggg gggggggcgc ngcgccaggc ggggcggggc gaggggcggg gcggggcgag
     6601 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     6661 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     6721 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     6781 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     6841 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     6901 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     6961 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     7021 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     7081 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     7141 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     7201 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     7261 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     7321 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agtgccggcg gctgtcgagg
     7381 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     7441 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     7501 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     7561 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     7621 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     7681 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     7741 gctggttatt gtgctgtctc atcattttgg caaagaattc gccactatgc cagagccagc
     7801 gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa
     7861 aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt
     7921 tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc
     7981 gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa
     8041 caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg
     8101 ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc
     8161 taagggttct gtatcaaaag gcgaggcggt cattaaagag ttcatgaggt ttaaagtcca
     8221 tatggaagga agcatgaatg gtcacgagtt tgagatagag ggagagggtg agggaaggcc
     8281 gtacgaaggg acgcaaactg cgaagttgaa ggtaacgaaa ggcggccctc tgccattctc
     8341 ttgggacatc ctttcaccgc aatttatgta cggctctagg gccttcacaa aacaccccgc
     8401 cgatattccg gattactata aacaaagttt tcctgagggc ttcaagtggg aaagagtaat
     8461 gaacttcgag gatgggggag ctgtgacggt tactcaagac actagcctgg aggacggtac
     8521 tcttatatac aaggtaaaac taagagggac gaattttccg ccagacggcc ccgtgatgca
     8581 aaaaaaaact atggggtggg aagcatccac cgagaggctg taccccgaag acggggtcct
     8641 gaagggtgat attaaaatgg cattaagatt aaaagatggc gggagatact tagctgactt
     8701 caagactaca tataaagcca aaaaacctgt acagatgcct ggagcttaca atgtggatcg
     8761 taagctggat attacatccc acaacgaaga ttataccgtg gtggagcagt acgagcgttc
     8821 agaggggcgt cactccaccg ggggtatgga tgaattatac aagagcggtc ttagaagcag
     8881 ggcgcaagcg tcaaattcag cggtagatgg aacagcaggt ccaggtagta ctggcagcag
     8941 aggatcctaa ctcgagattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     9001 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     9061 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     9121 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     9181 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     9241 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     9301 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     9361 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     9421 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     9481 gatccctcga cctgcagccc aagcttggcg taatgctgag ca
//
