LOCUS       sequence-252416-        7059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of FLAG-tagged mouse CD72c in animal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7059)
  TITLE     Tsubata CD72, SmB, and U1-C plasmids
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMXCD72cFLAG-ires-YFP
FEATURES             Location/Qualifiers
     source          1..7059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             345..761
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     691..713
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     729..745
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    771..1145
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1083..1099
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2220..2243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(2919..2921,2922..2924,2925..3638)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2964..2985)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3225..3244)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3572..3593
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3809..4278
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4408..4425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4579..5167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4659..4678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5331..6122,6123..6191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5954..5973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6192..6296)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6326..6919
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    join(6982..7059,1..280)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc
       61 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg
      121 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg
      181 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      241 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc
      301 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca
      361 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
      421 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
      481 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
      541 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
      601 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
      661 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
      721 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
      781 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
      841 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
      901 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
      961 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     1021 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     1081 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     1141 gactgccgga tcccagtgtg gtggtacggg aattcatggc tgacgctatc acgtatgcag
     1201 acctgcgctt tgtgaaagtg cccctgaaga acagcgcatc taaccatcta ggacaggact
     1261 gtgaggccta tgaagatggg gaactcacct acgagaacgt gcaagtgtct ccagtcccag
     1321 gagggccacc aggcttggct tcccctgcac tagcggacaa agcaggggtc gggtcagagc
     1381 aaccaactgc gacctggagc tctgtgaagt cgtctgctct caggcagatt ccccgctgtc
     1441 ctacagtctg cttgcaatac ttcttgcttg gccttctcgt gtcctgtctg atgttagggg
     1501 tggctgtcat ctgcctggga gttcgctatc tgcaggtgtc tcggcagttc caggagggga
     1561 ccaggatttg ggaagccacc aatagcagcc tgcagcagca gctcagggag aagataagtc
     1621 agctggggca gaaggaggtg gagcttcaga aggctcggaa agagctgatc tcgagccagg
     1681 acacattaca ggagaagcag aggactcacg aggacgctga gcagcaacta caagcctgcc
     1741 aggctgagag agcgaagacc aaggagaacc tgaaaactga ggaggagcag aggagggacc
     1801 tggaccagag gttgacaagc acacgggaga cactaaggcg cttcttctct gattcatcag
     1861 acacctgctg tccatgcgga tggattccat atcagaaaag gtgcttttac atctcacata
     1921 ccctcagaag tctggaggaa agccaaaaat actgcacatc tctgtcctcc aaattggcag
     1981 cattcaatga ccctcctaag tatttctatc aagtttctct gcccagcggc ttagaggagt
     2041 tgctagatcg tttgaagtca tattggatac aggagatgag caagaagtgg agctcatatt
     2101 gtgacatgat acaaatatat aacaagaagt gggcaagaac attttccgag tgtgcagagc
     2161 ttcacccctg catttgtgag ttggaggctt tcaggtttcc tgatgggatc catctgaacg
     2221 attacaagga tgacgacgat aagtgaaccg gatagcggcc gcgtcgagca gctgaagctt
     2281 aaaacagctc tggggttgta cccaccccag aggcccacgt ggcggctagt actccggtat
     2341 tgcggtaccc ttgtacgcct gttttatact cccttcccgt aacttagacg cacaaaacca
     2401 agttcaatag aagggggtac aaaccagtac caccacgaac aagcacttct gtttccccgg
     2461 tgatgtcgta tagactgctt gcgtggttga aagcgacgga tccgttatcc gcttatgtac
     2521 ttcgagaagc ccagtaccac ctcggaatct tcgatgcgtt gcgctcagca ctcaacccca
     2581 gagtgtagct taggctgatg agtctggaca tccctcaccg gtgacggtgg tccaggctgc
     2641 gttggcggcc tacctatggc taacgccatg ggacgctagt tgtgaacaag gtgtgaagag
     2701 cctattgagc tacataagaa tcctccggcc cctgaatgcg gctaatccca acctcggagc
     2761 aggtggtcac aaaccagtga ttggcctgtc gtaacgcgca agtccgtggc ggaaccgact
     2821 actttgggtg tccgtgtttc cttttatttt attgtggctg cttatggtga caatcacaga
     2881 ttgttatcat aaagcgaatt ggattgcggc cggccgcaat ggtgagcaag ggcgaggagc
     2941 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3001 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3061 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
     3121 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3181 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3241 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3301 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3361 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3421 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3481 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc
     3541 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3601 ccgggatcac tctcggcatg gacgagctgt acaagtaagt cgacgataaa ataaaagatt
     3661 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     3721 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     3781 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     3841 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     3901 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     3961 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     4021 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     4081 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     4141 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     4201 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     4261 gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag
     4321 tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4381 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4981 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg
     5281 gccggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5341 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5401 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5461 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5521 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5581 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5641 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5701 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5761 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5821 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5881 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5941 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6001 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6061 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6301 tttccccgaa aagtgccacc tgcataatga aagaccccac ctgtaggttt ggcaagctag
     6361 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gaaaagttca
     6421 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     6481 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
     6541 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     6601 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     6661 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     6721 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
     6781 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
     6841 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     6901 tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc
     6961 accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt ccgattgtct
     7021 agtgtctatg actgatttta tgcgcctgcg tcggtacta
//
