LOCUS       sequence-252418-        7035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse CD72c in animal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7035)
  TITLE     Tsubata CD72, SmB, and U1-C plasmids
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMXCD72c-ires-YFP
FEATURES             Location/Qualifiers
     source          1..7035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    475..832
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             897..1313
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1243..1265
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1281..1297
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1323..1697
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1635..1651
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(3447..3449,3450..3452,3453..4166)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3492..3513)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3753..3772)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4100..4121
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4337..4806
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4936..4953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5107..5695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5187..5206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5859..6650,6651..6719))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6482..6501
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6720..6824)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(6854..7035,1..412)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
       61 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      121 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      181 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      241 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      301 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      361 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      421 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      481 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      541 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      601 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      661 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      721 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      781 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      841 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      901 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
      961 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1021 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1081 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1141 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1201 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1261 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1321 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1381 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1441 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1501 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1561 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1621 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1681 gaccatcctc tagactgccg gatcccagtg tggtggtacg ggaattcatg gctgacgcta
     1741 tcacgtatgc agacctgcgc tttgtgaaag tgcccctgaa gaacagcgca tctaaccatc
     1801 taggacagga ctgtgaggcc tatgaagatg gggaactcac ctacgagaac gtgcaagtgt
     1861 ctccagtccc aggagggcca ccaggcttgg cttcccctgc actagcggac aaagcagggg
     1921 tcgggtcaga gcaaccaact gcgacctgga gctctgtgaa gtcgtctgct ctcaggcaga
     1981 ttccccgctg tcctacagtc tgcttgcaat acttcttgct tggccttctc gtgtcctgtc
     2041 tgatgttagg ggtggctgtc atctgcctgg gagttcgcta tctgcaggtg tctcggcagt
     2101 tccaggaggg gaccaggatt tgggaagcca ccaatagcag cctgcagcag cagctcaggg
     2161 agaagataag tcagctgggg cagaaggagg tggagcttca gaaggctcgg aaagagctga
     2221 tctcgagcca ggacacatta caggagaagc agaggactca cgaggacgct gagcagcaac
     2281 tacaagcctg ccaggctgag agagcgaaga ccaaggagaa cctgaaaact gaggaggagc
     2341 agaggaggga cctggaccag aggttgacaa gcacacggga gacactaagg cgcttcttct
     2401 ctgattcatc agacacctgc tgtccatgcg gatggattcc atatcagaaa aggtgctttt
     2461 acatctcaca taccctcaga agtctggagg aaagccaaaa atactgcaca tctctgtcct
     2521 ccaaattggc agcattcaat gaccctccta agtatttcta tcaagtttct ctgcccagcg
     2581 gcttagagga gttgctagat cgtttgaagt catattggat acaggagatg agcaagaagt
     2641 ggagctcata ttgtgacatg atacaaatat ataacaagaa gtgggcaaga acattttccg
     2701 agtgtgcaga gcttcacccc tgcatttgtg agttggaggc tttcaggttt cctgatggga
     2761 tccatctgaa ctgaaccgga tagcggccgc gtcgagcagc tgaagcttaa aacagctctg
     2821 gggttgtacc caccccagag gcccacgtgg cggctagtac tccggtattg cggtaccctt
     2881 gtacgcctgt tttatactcc cttcccgtaa cttagacgca caaaaccaag ttcaatagaa
     2941 gggggtacaa accagtacca ccacgaacaa gcacttctgt ttccccggtg atgtcgtata
     3001 gactgcttgc gtggttgaaa gcgacggatc cgttatccgc ttatgtactt cgagaagccc
     3061 agtaccacct cggaatcttc gatgcgttgc gctcagcact caaccccaga gtgtagctta
     3121 ggctgatgag tctggacatc cctcaccggt gacggtggtc caggctgcgt tggcggccta
     3181 cctatggcta acgccatggg acgctagttg tgaacaaggt gtgaagagcc tattgagcta
     3241 cataagaatc ctccggcccc tgaatgcggc taatcccaac ctcggagcag gtggtcacaa
     3301 accagtgatt ggcctgtcgt aacgcgcaag tccgtggcgg aaccgactac tttgggtgtc
     3361 cgtgtttcct tttattttat tgtggctgct tatggtgaca atcacagatt gttatcataa
     3421 agcgaattgg attgcggccg gccgcaatgg tgagcaaggg cgaggagctg ttcaccgggg
     3481 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3541 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3601 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct
     3661 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3721 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3781 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     3841 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     3901 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     3961 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4021 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     4081 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4141 tcggcatgga cgagctgtac aagtaagtcg acgataaaat aaaagatttt atttagtctc
     4201 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     4261 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     4321 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     4381 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     4441 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     4501 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     4561 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     4621 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     4681 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     4741 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     4801 ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa
     4861 atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc cggccgcaaa
     5821 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5881 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5941 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6001 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6061 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6121 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6181 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6241 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6301 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6361 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6421 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6481 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6541 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6601 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6661 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6721 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6781 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6841 gtgccacctg cataatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     6901 cattttgcaa ggcatggaaa aatacataac tgagaataga aaagttcaga tcaaggtcag
     6961 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     7021 ccggctcagg gccaa
//
