LOCUS       sequence-252426-       13869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Tan BS, Yang MC, Singh S, Chou YC, Chen HY, Wang MY, Wang YC, Chen 
            RH
  TITLE     LncRNA NORAD is repressed by the YAP pathway and suppresses lung and
            breast cancer metastasis by sequestering S100P.
  JOURNAL   Oncogene. 2019 Jul;38(28):5612-5626. doi: 10.1038/s41388-019-0812-8.
            Epub 2019 Apr 9.
  PUBMED    30967631
REFERENCE   2  (bases 1 to 13869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-0812-8"; journalName: "Oncogene"; date: 
            "2019-07"; volume: "38"; issue: "28"; pages: "5612-5626"
COMMENT     Sequence Label: pAS4.1w-tet-on-NORAD
FEATURES             Location/Qualifiers
     source          1..13869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    270..288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    305..323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    341..359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    377..395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    412..430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    448..466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        478..516
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        542..560
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2872..2883
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    6061..6178
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6227..6737
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6656..6674
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6749..7144
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             7196..7261
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(7268..7885,7886..7891,7892..7927,7928..7930,7931..7966,
                     7967..7969,7970..8005,8006..8014)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8020..8608
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8073..8093)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8491..8502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8680..8913
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8991..9112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9028..9047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9082..9101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9152..9287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9308..9326)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9336..9353)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9336..9352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9345..9367)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9494..9949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9581..9600)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9791..9812
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9975..10079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10080..10148,10149..10940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10298..10317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11111..11699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11600..11619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11853..11870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11987..12008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12023..12028,12029..12046,12047..12053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12061..12077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12066..12088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12085..12101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12085..12101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12120..12140
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12122..12140
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12168..12394
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12395..12575
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12622..12747
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13240..13473
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13658..13702
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gaggatccac
      121 tagtgaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      181 gggccagata tacgcgcgta tcacgaggcc ctttcgtctt cactcgagtt tactccctat
      241 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
      301 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      361 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      421 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      481 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagaatttt
      541 gtaatacgac tcactatagg gcggccggga attcgtcgac tggatccggt accttcgaac
      601 cttagggatg gatccagttc cggtccggca gagatcgcgg agagacgcag aacgcagccc
      661 gctcctccag ggccctccag gccctccggc cccgggccgg cgggtgaact ggggggcccc
      721 gggacaggcc gagccctctg ccctgcagat aacggaggcc tctgctgtgg ctgcccactg
      781 gctgtgcccg cccactggct gtgcccagac cttgaagccg cagcgaacct ctctttccca
      841 ccccacctcg gtgactaatg gcggccgtgg cgtctcccag cccggacccc gccggcaccc
      901 gggtctcccg acccaagcct cgacgaaacc cccgcagagc cgccgggacg cagcgccttt
      961 gggcggcgct gggcgtggtg ggccgggaag tatggcggca gctcgaacgc cgcgcggcgg
     1021 aggccattaa ggcgtggacg gcccgggaag gcggcctagg gacgcaagca ggctcggccg
     1081 cctctttagg ccacggagcc gcgcagatcc ggttcccggg tgaccactct gtcgccattg
     1141 ggcgagacct acctagtcct gacgacaacg gacaaaggcc ttaaggggcc tggaaggtga
     1201 gcgaagtccc gaacgacgac gggtggaacg gttagcggcc atcgggcggt tggtcttcat
     1261 tctaccagac tttgctgtcg gaagagagaa atggtagaat gacaggccac gtttggcccg
     1321 ttggaaatgc ccaccaccct ctgggaagat ttactggccg tttatggaag gcctgtgtat
     1381 ataatatgaa aaagctgctc tcaactccac cccaaccttt taatagaaaa catttgtcac
     1441 atctagccct tctagatgga aagaggttgc cgacgtatga taaaatagag ttagaaagtt
     1501 acacatcttg taaattctca tttgtttaaa agaaatcata gaaaatacat gtcttctgga
     1561 gatgactttt ggaaatggag ttgttaagac ggcctctgga agcgatacgt ccacgtttgt
     1621 taagtgggtt agatgacatg gagctggaag acctgagaag gaagagaaga aggttctatg
     1681 ctagactggt catatttaga agacattttc atattctatc cattgttttg tgtgcatttt
     1741 attcctcact actgtgtata tagttgacaa tgctaagctt ttttgaaatg tctcttcttt
     1801 ttagatgttc tgaagtgcct gatatgttaa aattagaggt agcaaaatca cattttgtaa
     1861 ataccttttt gttacaattc ataggaaata tttttggggg ggaatggcca aatcacctgt
     1921 tgagtaatac tcattgtgtt tgtgcagtgg ttcaggggag gagagaggag ggggaggtgc
     1981 agagagctct atgccatcct gtttacagcg aggcaagatg aatcattatg tctgtgcatt
     2041 ttgttttact tatctgtgta tatagtgtac ataaaggaca gacgagtcct aattgacaac
     2101 atctagtctt tctggatgtt aaagaggttg ccagtgtatg acaaaagtag agttagtaaa
     2161 ctaatatatt ttgtacattt tgttttacaa gtcctaggaa agattgtctt ctgaaaattt
     2221 gatgtcttct gggttgatgg agatgggaag ggttctaggc cagaatgttc acatttggaa
     2281 gactctttca aattataact gttgttacat gtttgcagtt tattcaagac tgctgtatac
     2341 atagtagaca aattaactcc ttacttgaaa catctagtct atctagatgt ttagaagtgc
     2401 ccgatgtatg ttaaatgtat aggtagtaaa ataccacttt gtaaatatct ttttgctaaa
     2461 attcatagga aatgcttttg gaaattgaat tgtgaagcca cctttgtgaa cagtatagta
     2521 atgtctatac ttgttcaata gtttagagga ggtaggaggg aagaaattgc aaaaggtaat
     2581 attactagtg tgttcatact tggacatttt cagacaccat ttttctatat gttttgtgca
     2641 ttttgttttg ctctgtatat agtatatata atggacaaat agtcctaatt tttcaacatc
     2701 tagtctctag atgttaaaga ggttgccagt gtatgacaaa ggagtaaaat tagcatattt
     2761 tgtacacttt gtgttgaaat tcgtaggaaa acttgtcttc tgtaaagact tttgcatagg
     2821 aatttgtttg accatctcta agcattacac gtgcctgtac ttgtccactg gattgaaggc
     2881 agagaaggaa gggaggaggg aatgattcaa ggccaaaatg gccacattta gaagatacct
     2941 cagatgataa ccattgttat gtgtgtgcaa ttttatttaa cagtgctgtg tatgtggtgg
     3001 acaagttata tgaaatatct agtctttcta gatatttgga agtgcttgat gtatttaaaa
     3061 gtggtagtag aataacactt tgtaaatagc ttttaaaaac tgatgggaaa tgctgtttgg
     3121 aagtggaatt gttgaaccac ctgggaggtg ggagggaaga aattgcaaat ggtgttttgc
     3181 cattgtttat tagaaaattt cagcttaatc cattgtgtat atgttacatg catttcattt
     3241 aactttgcta tactgtatat attgtatata taacggacaa attagtcccg attttataat
     3301 atctagtctc tagatattaa agaggttgcc aatgtatgac agaagtagag ttagtaaact
     3361 aacacatttt gtacactttg ttaaaatttg tagaaaggct gtcttctgaa aaggactttt
     3421 ggaagtgaga taacatcagc tctaagtgac acgtgcctat atccatcagg ttggtggtgg
     3481 agaggagttg gaaggaatga agggttctag accagaatgt tcgtatttag aagacactat
     3541 cagatataac cattgttaca tgtgtgtagt ttattcaacc ctactgtgta tatagcggac
     3601 aaacttaagt ccttatttga aacatctagt ctttctagat gtttagaagt gcacaaagta
     3661 tgttaaaagt agaggtagta aataacacat tttgtagcta tccttttgat atgaaatatt
     3721 gtcttggaaa ttgatcaatt ctctgagcag tacccatttt gatatttgtg ctggttcagg
     3781 gggaaggagg agcacaaagt gcaaagggct ttctaccagt gtccagtgtg tttatgagga
     3841 ggcacattga ccattgtccc ttatgtctgc attttcattt actgtgctgt gtatatagtg
     3901 tatataagcg gacataggag tcctaattta cgtctagtcg atgttaaaaa ggttgccagt
     3961 atatgacaaa agtagaatta gtaaactact acattgagta cactttgtgt taaaattcat
     4021 agggaagact tcttaaaaac aagtgaaatt gttaaaaccc cccctaagca ttacagatgg
     4081 cttatagctg tccacggggt tggtagaggt gggaaaggga agggttctag gccagaatgt
     4141 tcctatttag aagacactca aattacagtc tgtgttatgt atgtatacca tttattcaat
     4201 gctactgtgt atataatgga aaacttaagt ccagtttgaa acatctagtc tttctaggtg
     4261 tttaaaagtg tacaacggcc tgtcgcagtg gcgcatgcct gtaatcccag cactttggga
     4321 ggccgaggca ggcggatcac gaggtcaaga gatcaggacc atcttggcca acatggtgaa
     4381 accccatctt tactaaaaat acaaaaatta gctggtcgtg gtggtgccca cctgtagccc
     4441 cagttactcg agaggctgag gcaggagaat cgcttgaact tgggaggcgg aagttgcagt
     4501 gagccaagat cgcaccactg cactccagcc tggcgacaga gcgaggctcc gtttcaaaaa
     4561 aaaaagtgca caatgtaggt taacagtaga gggcttaagt aacacccctc taagcatttg
     4621 ttttcagtac ttcctaggag tggttgcatt tgggaatgga attgttaaaa cttgatgctt
     4681 aggagcgaat gcagactatt cattgggtgt ttggggtggg ggaagggggg gtgggcagag
     4741 gaggtatgca gggagagggg ttctgtgctc ctgagattag ttcagatggt ctaaccattg
     4801 ttctatatgt gcattttagt taatattgtg tattaaagga taagtcttaa tgctcaaagt
     4861 atgttaaaaa tagatgtagt aaatcagtcc ctttgtgaat gtccttttgt tagtttttag
     4921 gaaggcctgt cctctgggag tgacctttat tagtccaccc cttggagcta gacatcctgt
     4981 acttagtcac ggggatggtg gaagagggag aagaggaagg gtgaagggaa gggctctttg
     5041 ctagtatctc catatctaga cgatggtttt agatgataac cacaggtcta caagagcgtt
     5101 tttagtaaag tgcctgtgtt cattgtggac aaagttatta ttttgcaaca tctaagcttt
     5161 acgaatgggg tgacaactta tgataaaaac tagagctagt gaattagcct atttgtaaat
     5221 acctttgtta taattgatag gatacatctt ggacatggaa ttgttaagcc acctctgagc
     5281 agtgtatgtc aggacttgtt cattaggttg gcagcagagg ggcagaagga attatacagg
     5341 tagagatgta tgcagatgtg tccatatatg tccatattta cattttgata gccattgatg
     5401 tatgcatctc ttggctgtac tataagaaca cattaattca atggaaatac actttgctaa
     5461 tattttaatg gtatagatct gctaatgaat tctcttaaaa acatactgta ttctgttgct
     5521 gtgtgtttca ttttaaattg agcattaagg gaatgcagca tttaaatcag aactctgcca
     5581 atgcttttat ctagaggcgt gttgccattt ttgtcttata tgaaatttct gtcccaagaa
     5641 aggcaggatt acatcttttt tttttttttt agcagtttga gttggtgtag tgtattcttg
     5701 gttatcagaa tactcatata gctttgggat tttgaattgg taaatattca tgatgtgtga
     5761 aaaatcatga tacatactgt acagtctcag tcccataaaa ttggatgttg tgcctacaca
     5821 caggatctag aagaatatgt caaactataa actgcttgtg attgtgaatg actttgttct
     5881 ttgcttgtgt ttttcaattt cctataatgc acatactaac ttttaaaaaa taaaggttat
     5941 tttaaaagcc tgtattaagt cgacgcgtat ccacgtggcg atcgctagcg atcgcctgca
     6001 ggacgcgtat gcatgtacag tttaaacgac ctcgagacaa atggcagtat tcatccacaa
     6061 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     6121 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     6181 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     6241 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     6301 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     6361 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     6421 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     6481 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     6541 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     6601 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     6661 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     6721 ccgacctctc tccccagggg gatccaccat ggccaagcct ttgtctcaag aagaatccac
     6781 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     6841 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     6901 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     6961 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     7021 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     7081 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     7141 gggcctgcag ctgcagcctg caggatctgg cgaagccaga cacaaacaga aaattgtgaa
     7201 acagactttg aattttgacc ttctcaagtt ggcgggagat gtggagtcca acccagggcc
     7261 cgcaagcatg tctagactgg acaagagcaa agtcataaac ggcgctctgg aattactcaa
     7321 tggagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     7381 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     7441 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     7501 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
     7561 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     7621 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     7681 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     7741 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     7801 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     7861 gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga
     7921 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
     7981 tgacgatttt gaccttgaca tgctccccgg gtaacgcgta atcaacctct ggattacaaa
     8041 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8101 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8161 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8221 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8281 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8341 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8401 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8461 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8521 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8581 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     8641 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8701 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8761 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8821 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8881 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     8941 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9001 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9061 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9121 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9181 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9241 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     9301 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9361 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9421 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9481 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9721 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9781 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9841 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9901 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     9961 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10021 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10081 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10141 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10201 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10261 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10321 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10381 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10441 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10501 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10561 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10621 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10681 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10741 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10801 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10861 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10921 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10981 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11041 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11101 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11161 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11221 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    11281 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11341 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11401 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11461 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11521 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11581 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11641 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11701 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11761 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11821 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11881 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    11941 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    12001 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    12061 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    12121 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
    12181 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
    12241 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
    12301 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
    12361 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
    12421 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    12481 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    12541 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    12601 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    12661 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    12721 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    12781 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    12841 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    12901 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    12961 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    13021 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    13081 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    13141 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    13201 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    13261 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    13321 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    13381 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    13441 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    13501 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    13561 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    13621 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    13681 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    13741 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    13801 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    13861 aaccccgag
//
