LOCUS       sequence-252431-        9861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes crippled pSFFV driving expression of mAzamiGreen with an 
            mScarlet normalization in a PiggyBac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0523
FEATURES             Location/Qualifiers
     source          1..9861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        540..743
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     694..714
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      765..774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             771..1799
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             771..1799
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    1817..2024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2231..2293
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(2344..2362)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3125..3144
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3209..3228
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4142..4161
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4142..4160
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4190..4224
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        4831..5238
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5261..5281
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5297..5968
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             5297..5968
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     polyA_signal    5995..6219
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6467..6770
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6772..7048
                     /label=chicken beta-actin promoter
     intron          7049..8065
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7989..8011
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8073..8092
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             8126..8503
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(9377..9396)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9442..9497
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9496..9515)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg cttgatgtgg
       61 acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa ccgggctctg
      121 tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca ggaaggaaag
      181 gttgcatgct ggacacacta accttacagc ggtgggctct atggggtacc tacgtaggta
      241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      721 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatagccacc atgggtaaaa
      781 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt
      841 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      901 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      961 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     1021 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     1081 tgcctgaaac cgaactgccc gctgttctgc aaccggtcgc ggaggccatg gatgcgatcg
     1141 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     1201 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     1261 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     1321 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     1381 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     1441 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1501 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     1561 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1621 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1681 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1741 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataac
     1801 tcgagcgacg cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2041 agtatcgata ctagttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt
     2101 tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag
     2161 tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg atatacagac
     2221 cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat
     2281 tatctttcta gggttaatct agtatacgcg tatgcggccg cttaattaat ccagcttttg
     2341 ttccctttag tgagggttaa ttccgagcgg ccgcgtgtta caaccaatta accaattctg
     2401 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2461 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2521 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2581 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2641 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
     2701 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     2761 gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     2821 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     2881 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     2941 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3001 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     3061 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     3121 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     3181 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     3241 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3301 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     3361 atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgccaa
     4141 ttaatacgac tcactatagg gcgaattggg gcgcgccatt ctagattaac cctagaaaga
     4201 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     4261 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     4321 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     4381 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     4441 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa
     4501 ctagaatgct agcatgggcc cagatctcca tgggccctga attcgcatct agactgaact
     4561 ggccgataat tgcagacgag gagcatcgcc cttccccggc cctcaggtaa gaggaccaaa
     4621 taccgtagcc gtttccaatt tcagtccttt agcgccacct ggtgctaact actctatcac
     4681 gcttttatcc aataactacc tttgtaaatt tcctttcaaa agttctggcc gggcgcggtg
     4741 gcaacgccga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     4801 cccacctgta ggtttggcaa gctagctgca gtaacgccat tttgcaaggc atggaaaaat
     4861 accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata gctaacgttg
     4921 ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca agaacagatg
     4981 gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag atatggccca
     5041 accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag gacctgaaat
     5101 gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt
     5161 ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc agtcctccga
     5221 cagactgagt cgcccgggag atcgccgccg cagatctatg cccaagaaga agaggaaggt
     5281 ggaggacgcc ggttctgtga gcgtgatcaa gcccgagatg aagatcaagc tgtgcatgag
     5341 gggcaccgtg aacggccaca acttcgtgat cgagggcgag ggcaagggca acccctacga
     5401 gggcacccag atcctggacc tgaacgtgac cgagggcgcc cccctgccct tcgcctacga
     5461 catcctgacc accgtgttcc agtacggcaa cagggccttc accaagtacc ccgccgacat
     5521 ccaggactac ttcaagcaga ccttccccga gggctaccac tgggagagga gcatgaccta
     5581 cgaggaccag ggcatctgca ccgccaccag caacatcagc atgaggggcg actgcttctt
     5641 ctacgacatc aggttcgacg gcaccaactt cccccccaac ggccccgtga tgcagaagaa
     5701 gaccctgaag tgggagccca gcaccgagaa gatgtacgtg gaggacggcg tgctgaaggg
     5761 cgacgtgaac atgaggctgc tgctggaggg cggcggccac tacaggtgcg acttcaagac
     5821 cacctacaag gccaagaagg aggtgaggct gcccgacgcc cacaagatcg accacaggat
     5881 cgagatcctg aagcacgaca aggactacaa caaggtgaag ctgtacgaga acgccgtggc
     5941 caggtactcc atgctgccca gccaggccgg atcctaactc gagcgacgcc tcgactgtgc
     6001 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6061 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6121 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6181 acaatagcag gcatgctggg gatgcggtgg gctctatggg ctggttctgt atgggcacag
     6241 acaacctaac taatttcctg gccctcaagg tgacttgctt gaacttgcca ccttcgccac
     6301 tagggggcag cattggttta cacagggtga acaaacgttc aattctaaac taatattctt
     6361 tggtgggaaa gtgtgttcat tttggtttgt tttgaagaag tcacttcata cctttgaact
     6421 ggggttaagc tggttaactc ccaaaaacgc catatatgga gttccgcgtt acataactta
     6481 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     6541 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     6601 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6661 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6721 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     6781 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     6841 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcnc
     6901 aggcggggcg gggcggggsg rggggcgggg cggggcgagg cggagaggtg cggcggcagc
     6961 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     7021 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     7081 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     7141 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     7201 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     7261 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     7321 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     7381 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     7441 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     7501 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     7561 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     7621 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     7681 ggggcgcggc ggcccccgga gtgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     7741 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     7801 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     7861 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     7921 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     7981 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     8041 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     8101 tcattttggc aaagaattcg ccactatgcc agagccagcg aagtctgctc ccgccccgaa
     8161 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg
     8221 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga
     8281 caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga
     8341 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc
     8401 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt
     8461 gtccgagggt actaaggcca tcaccaagta caccagcgct aagggttctg tatcaaaagg
     8521 cgaggcggtc attaaagagt tcatgaggtt taaagtccat atggaaggaa gcatgaatgg
     8581 tcacgagttt gagatagagg gagagggtga gggaaggccg tacgaaggga cgcaaactgc
     8641 gaagttgaag gtaacgaaag gcggccctct gccattctct tgggacatcc tttcaccgca
     8701 atttatgtac ggctctaggg ccttcacaaa acaccccgcc gatattccgg attactataa
     8761 acaaagtttt cctgagggct tcaagtggga aagagtaatg aacttcgagg atgggggagc
     8821 tgtgacggtt actcaagaca ctagcctgga ggacggtact cttatataca aggtaaaact
     8881 aagagggacg aattttccgc cagacggccc cgtgatgcaa aaaaaaacta tggggtggga
     8941 agcatccacc gagaggctgt accccgaaga cggggtcctg aagggtgata ttaaaatggc
     9001 attaagatta aaagatggcg ggagatactt agctgacttc aagactacat ataaagccaa
     9061 aaaacctgta cagatgcctg gagcttacaa tgtggatcgt aagctggata ttacatccca
     9121 caacgaagat tataccgtgg tggagcagta cgagcgttca gaggggcgtc actccaccgg
     9181 gggtatggat gaattataca agagcggtct tagaagcagg gcgcaagcgt caaattcagc
     9241 ggtagatgga acagcaggtc caggtagtac tggcagcaga ggatcctaac tcgagattca
     9301 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     9361 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     9421 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     9481 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     9541 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     9601 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     9661 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     9721 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     9781 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     9841 agcttggcgt aatgctgagc a
//
