LOCUS       sequence-252433-        7545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Httex1 (without proline-rich region) fused to ymsfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Albakri MB, Jiang Y, Genereaux J, Lajoie P
  TITLE     Polyglutamine toxicity assays highlight the advantages of mScarlet 
            for imaging in Saccharomyces cerevisiae.
  JOURNAL   F1000Res. 2018 Aug 10;7:1242. doi: 10.12688/f1000research.15829.2. 
            eCollection 2018.
  PUBMED    30631438
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2018-08-10"; volume: "7:1242. doi"; pages: " 
            10.12688/f1000research.15829.2. eCollection 2018"
COMMENT     Sequence Label: 25Q-ymsfGFP
FEATURES             Location/Qualifiers
     source          1..7545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..44
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        26..44
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        63..504
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    63..180
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     475..498
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             536..559
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1442..1689
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1458..1476)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1707..1726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1708..1726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1736..1753)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1736..1752)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1745..1767)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1893..2348
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1980..1999)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2190..2211
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2649..3056
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3057..4151
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(4645..4664)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4764..4786
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4824..4842)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4884..5387
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        5424..5528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5529..5597,5598..6389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5747..5766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6560..7148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7049..7068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7302..7319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7436..7457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7472..7477,7478..7495,7496..7502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7510..7526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7515..7537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(7534..7545,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7534..7545,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
       61 tccggattag aggccgccga gcgggtgaca gccctccgaa ggaagactct cctccgtgcg
      121 tcttcgtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg
      181 aacaataaag attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa
      241 cctggcccca caaaccttca aatgaacgaa tcaaattaac aaccatagga tgataatgcg
      301 attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta
      361 ttaacagata tataaatgca aaaactgcat aaccacttta actaatactt tcaacatttt
      421 cggtttgtat tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata
      481 cctctatact ttaacgtcaa ggagaaaaaa ccccggattc tagaactacg ccatggacta
      541 caaggacgac gatgacaagc tggcgaccct ggaaaagctg atgaaggcct tcgagtccct
      601 caaaagcttc caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
      661 gcaacaacag cagcaacagc aacaactgca gcccggggga tccactagtg tatccaaagg
      721 tgaagaattg tttactggtg ttgttcctat tttagttgaa ttagacggtg acgttaatgg
      781 tcataagttt agtgttagag gtgaaggtga aggtgacgca actaacggta aattgacatt
      841 gaagtttatt tgtactactg gtaaattgcc agttccatgg ccaaccttgg ttaccacttt
      901 aacttatggt gttcaatgct tctcaagata ccctgatcat atgaaaagac acgacttttt
      961 caagagtgcc atgccagaag gttacgtaca agaaagaact atatctttta aagatgacgg
     1021 tacatacaag accagagctg aagtaaaatt cgaaggtgac acattggtca acagaatcga
     1081 attgaagggt atcgatttca aggaagacgg taacatcttg ggtcataaat tagaatacaa
     1141 tttcaactca cacaatgttt acatcactgc tgataagcaa aagaatggta tcaaagcaaa
     1201 cttcaagatc agacataacg ttgaagatgg ttccgtacaa ttagctgacc actatcaaca
     1261 aaatacacct attggtgacg gtcctgtttt gttaccagac aaccattact tgtctaccca
     1321 atcaaaattg tccaaagatc caaacgaaaa gagagaccat atggtcttgt tggaatttgt
     1381 cactgccgct ggtatcactc acggtatgga tgaattgtat aagtaggtcg acgacctcga
     1441 gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
     1501 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     1561 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     1621 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     1681 ttaatttgcg gccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     1741 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1801 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     1861 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
     1921 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1981 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2041 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2101 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2161 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2221 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2281 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     2341 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2401 tcgaccggtc gaggagaact tctagtatat ctacatacct aatattattg ccttattaaa
     2461 aatggaatcc caacaattac atcaaaatcc acattctctt caaaatcaat tgtcctgtac
     2521 ttccttgttc atgtgtgttc aaaaacgtta tatttatagg ataattatac tctatttctc
     2581 aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga aatatcttga
     2641 ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
     2701 aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca
     2761 aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt
     2821 caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag
     2881 aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat
     2941 ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc
     3001 ttacctttta catttcagca atatatatat atatatttca aggatatacc attctaatgt
     3061 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg
     3121 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg
     3181 aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg
     3241 cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat
     3301 ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat
     3361 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa
     3421 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta
     3481 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat
     3541 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg
     3601 agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat
     3661 ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc
     3721 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta
     3781 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt
     3841 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg
     3901 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta
     3961 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag
     4021 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt
     4081 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
     4141 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
     4201 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
     4261 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca
     4321 ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat
     4381 taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa
     4441 ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat
     4501 aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat
     4561 ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac
     4621 gtagtcgata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4861 gataataatg gtttcttagg acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt
     4921 ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt
     4981 ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa
     5041 caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa
     5101 aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt
     5161 ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca
     5221 ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc
     5281 ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt taatttatat
     5341 atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa aaggacccag
     5401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5461 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5761 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6241 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6421 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7201 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7261 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7321 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7381 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7441 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7501 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagct
//
