LOCUS       sequence-252445-        7521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Httex1 (without proline-rich region) fused to ymScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Albakri MB, Jiang Y, Genereaux J, Lajoie P
  TITLE     Polyglutamine toxicity assays highlight the advantages of mScarlet 
            for imaging in Saccharomyces cerevisiae.
  JOURNAL   F1000Res. 2018 Aug 10;7:1242. doi: 10.12688/f1000research.15829.2. 
            eCollection 2018.
  PUBMED    30631438
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2018-08-10"; volume: "7:1242. doi"; pages: " 
            10.12688/f1000research.15829.2. eCollection 2018"
COMMENT     Sequence Label: 25Q-ymScarlet
FEATURES             Location/Qualifiers
     source          1..7521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     60..80
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        62..80
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        99..540
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    99..216
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     511..534
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             572..595
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1454..1701
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1470..1488)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1719..1738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1720..1738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1748..1765)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1748..1764)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1757..1779)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1905..2360
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1992..2011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2202..2223
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2661..3068
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3069..4163
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(4657..4676)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4776..4798
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4836..4854)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4896..5399
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        5436..5540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5541..5609,5610..6401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5759..5778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6572..7160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7061..7080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7314..7331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7448..7469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7484..7489,7490..7507,7508..7514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
       61 caattaaccc tcactaaagg gaacaaaagc tggagctccg gattagaggc cgccgagcgg
      121 gtgacagccc tccgaaggaa gactctcctc cgtgcgtctt cgtcttcacc ggtcgcgttc
      181 ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta
      241 gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg
      301 aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg
      361 gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata aatgcaaaaa
      421 ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact tcttattcaa
      481 atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag
      541 aaaaaacccc ggattctaga actacgccat ggactacaag gacgacgatg acaagctggc
      601 gaccctggaa aagctgatga aggccttcga gtccctcaaa agcttccaac agcagcaaca
      661 gcaacaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca
      721 actgcagccc gggggatcca ctagtgtttc taaaggtgaa gcagttatta aggaattcat
      781 gagattcaag gtacacatgg aaggttctat gaatggtcac gaatttgaaa ttgaaggtga
      841 aggtgaaggt agaccatatg aaggtactca aactgctaag ttgaaggtta ctaaaggtgg
      901 tccattgcca ttttcttggg atattttgtc tccacaattc atgtacggtt ctagagcttt
      961 tacaaaacat ccagcagata ttccagatta ctacaagcaa tcattcccag aaggttttaa
     1021 atgggaaaga gttatgaact tcgaagatgg tggtgcagtt actgttacac aagatacttc
     1081 tttggaagat ggtacattga tctataaggt taagttgaga ggtactaatt ttccaccaga
     1141 tggtccagtt atgcaaaaga aaactatggg ttgggaagct tcaacagaaa gattgtaccc
     1201 agaagatggt gttttgaagg gtgacattaa gatggcattg agattgaagg atggtggtag
     1261 atatttggct gatttcaaga ctacatacaa ggctaagaaa ccagttcaaa tgccaggtgc
     1321 ttacaacgtt gatagaaagt tggatattac ttctcataat gaagattaca cagttgttga
     1381 acaatatgaa agaagtgaag gtagacacag tacaggtggt atggatgaat tatacaaatg
     1441 agtcgacctc gagtcatgta attagttatg tcacgcttac attcacgccc tccccccaca
     1501 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     1561 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     1621 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     1681 cgctcgaagg ctttaatttg cggccggtac ccaattcgcc ctatagtgag tcgtattacg
     1741 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1801 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1861 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
     1921 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1981 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     2041 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2101 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2161 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2221 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     2281 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2341 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2401 tcacaccgca tatcgaccgg tcgaggagaa cttctagtat atctacatac ctaatattat
     2461 tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc ttcaaaatca
     2521 attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata ggataattat
     2581 actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg cctgattcaa
     2641 gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat gcaagagttc
     2701 gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat
     2761 cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc
     2821 atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg
     2881 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt
     2941 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact
     3001 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata
     3061 ccattctaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag
     3121 aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca
     3181 agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac
     3241 ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg
     3301 gtggtcctaa atggggtacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta
     3361 aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag
     3421 acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat
     3481 tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg
     3541 atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg
     3601 ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct
     3661 cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgaattc cctacattga
     3721 aggttcaaca tcaattgatt gattctgccg ccatgatcct agttaagaac ccaacccacc
     3781 taaatggtat tataatcacc agcaacatgt ttggtgatat catctccgat gaagcctccg
     3841 ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga
     3901 acaccgcatt tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata
     3961 aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact
     4021 tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca
     4081 gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag
     4141 aagttaagaa aatccttgct taaaaagatt ctcttttttt atgatatttg tacataaact
     4201 ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg
     4261 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg
     4321 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt
     4381 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag
     4441 aaaatcatga aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa
     4501 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc
     4561 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
     4621 ggccttaacg acgtagtcga tatggtgcac tctcagtaca atctgctctg atgccgcata
     4681 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4741 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4801 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4861 ggttaatgtc atgataataa tggtttctta ggacggatcg cttgcctgta acttacacgc
     4921 gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta
     4981 tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga
     5041 aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt
     5101 attagacaag aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa
     5161 aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa
     5221 tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc
     5281 ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt
     5341 tttaatttat atatttatat taaaaaattt aaattataat tatttttata gcacgtgatg
     5401 aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5521 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5581 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5641 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5701 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5761 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5821 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5881 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5941 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6001 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6061 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6121 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6181 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6241 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6301 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6361 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6421 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6481 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6541 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6601 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6661 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6721 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6781 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6841 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6901 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6961 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7021 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7081 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7141 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7201 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7261 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7321 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7381 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7441 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7501 cggctcgtat gttgtgtgga a
//
