LOCUS       sequence-252450-        5256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORFeus expression, mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA027
FEATURES             Location/Qualifiers
     source          1..5256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4958..4981
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4982..5047
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggccgcgttt aaacttaatt aagccaccat gggcaagaag cagaaccgca agaccggcaa
       61 cagcaagacc cagagcgcca gccccccccc caaggagcgc agcagcagcc ccgccaccga
      121 gcagagctgg atggagaacg acttcgacga gctgcgcgag gagggcttcc gccgcagcaa
      181 ctacagcgag ctgcgcgagg acatccagac caagggcaag gaggtggaga acttcgagaa
      241 gaacctggag gagtgcatca cccgcatcac caacaccgag aagtgcctga aggagctgat
      301 ggagctgaag accaaggccc gcgagctgcg cgaggagtgc cgcagcctgc gcagccgctg
      361 cgaccaattg gaggagcgcg tgagcgccat ggaggacgag atgaacgaga tgaagcgcga
      421 gggcaagttc cgcgagaagc gcatcaagcg caacgagcag agcctgcagg agatctggga
      481 ctacgtgaag cgccccaacc tgcgcctgat cggcgtgccc gagagcgacg tggagaacgg
      541 caccaagctg gagaacaccc tgcaggacat catccaggag aacttcccca acctggcccg
      601 ccaggccaac gtgcagatcc aggagatcca gcgcaccccc cagcgctaca gcagccgccg
      661 cgccaccccc cgccacatca tcgtgcgctt caccaaggtg gagatgaagg agaagatgct
      721 gcgcgccgcc cgcgagaagg gccgcgtgac cctgaagggc aagcccatcc gtctcaccgc
      781 cgacctgagc gccgagaccc tgcaggcccg ccgcgagtgg ggccccatct tcaacatcct
      841 gaaggagaag aacttccagc cccgcatcag ctaccccgcc aagctgagct tcatcagcga
      901 gggcgagatc aagtacttca tcgacaagca gatgctgcgc gacttcgtga ccacccgccc
      961 cgccctgaag gagctgctga aggaggccct gaacatggag cgcaacaacc gctaccagcc
     1021 cctgcagaac cacgccaaga tgtaaagacc atcgagacta ggaagaaact gcatcaacta
     1081 atgagcaaaa tcaggcgcgc ccatcataat gaccggcagc aacagccaca tcaccatcct
     1141 gaccctgaac atcaacggcc tgaacagcgc catcaagcgc caccgcctgg ccagctggat
     1201 caagagccag gaccccagcg tgtgctgcat ccaggagacc cacctgacct gccgcgacac
     1261 ccaccgcctg aagatcaagg gctggcgcaa gatctaccag gccaacggca agcagaagaa
     1321 ggccggcgtg gccatcctgg tgagcgacaa gaccgacttc aagcccacca agatcaagcg
     1381 cgacaaggag ggccactaca tcatggtgaa gggcagcatc cagcaggagg agctgaccat
     1441 cctgaacatc tacgccccca acaccggtgc cccccgcttc atcaagcagg tgctgagcga
     1501 cctgcagcgc gacctggaca gccacaccct gatcatgggc gacttcaaca cccccctgag
     1561 caccctggac cgcagcaccc gccagaaggt gaacaaggac acccaggagc tgaacagcgc
     1621 cctgcaccag gccgacctga tcgacatcta ccgcaccctg caccccaaga gcaccgagta
     1681 caccttcttc agcgcccccc accacaccta cagcaagatc gaccacatcg tgggcagcaa
     1741 ggccctgctg agcaagtgca agcgcaccga gatcatcacc aactacctga gcgacgacag
     1801 cgccatcaag ctggagctgc gcatcaagaa cctgacccag agccgcagca ccacctggaa
     1861 gctgaacaac ctgctgctga acgactactg ggtgcacaac gagatgaagg ccgagatcaa
     1921 gatgttcttc gaaaccaacg agaacaagga caccacctac cagaacctgt gggacgcctt
     1981 caaggccgtg tgccgcggca agttcatcgc cctgaacgcc tacaagcgca agcaggagcg
     2041 cagcaagatc gacaccctga ccagccagct gaaggagctg gagaagcagg agcagaccca
     2101 cagcaaggcc agccgccgcc aggagatcac caagatccgc gccgagctga aggagatcga
     2161 gacccagaag accctgcaga agatcaacga gtcgcgaagc tggttcttcg agcgcatcaa
     2221 caagatcgac cgccccctgg cccgcctgat caagaagaag cgcgagaaga accagatcga
     2281 caccatcaag aacgacaagg gcgacatcac caccgacccc accgagatcc agaccaccat
     2341 ccgcgagtac tacaagcacc tgtacgccaa caagctggag aacctggagg agatggacac
     2401 cttcctggac acctacaccc tgccccgcct gaaccaggag gaggtggaga gcctgaaccg
     2461 ccccatcacc ggcagcgaga tcgtggccat catcaacagc ctgcccacca agaagagccc
     2521 cggccccgac ggcttcaccg ccgagttcta ccagcgctac aaggaggagc tggtgccctt
     2581 cctgctgaag ctgttccaga gcatcgagaa ggagggcatc ctgcccaaca gcttctacga
     2641 ggccagcatc atcctgatcc ccaagcccgg ccgcgacacc accaagaagg agaacttccg
     2701 ccccatcagc ctgatgaaca tcgacgccaa gatcctgaac aagatcctgg ccaaccgcat
     2761 ccagcagcac atcaagaagc tgatccacca cgaccaggtg ggcttcatcc ccgggatgca
     2821 gggctggttc aacatccgca agagcatcaa cgtgatccag cacatcaacc gcgccaagga
     2881 caagaaccac atgatcatca gcatcgacgc cgagaaggcc ttcgacaaga tccagcagcc
     2941 cttcatgctg aagaccctga acaagctggg catcgacggc acctacttca agatcatccg
     3001 cgccatctac gacaagccca ccgccaacat catcctgaac ggccagaagc tggaggcctt
     3061 ccccctgaag accggcacgc gtcagggctg ccccctgagc cccctgctgt tcaacatcgt
     3121 gctggaggtg ctggcccgcg ccatccgcca ggagaaggag atcaagggca tccagctggg
     3181 caaggaggag gtgaagctga gcctgttcgc ctacgacatg atcgtgtacc tggagaaccc
     3241 catcgtgagc gcccagaacc tgctgaagct gatcagcaac ttcagcaagg tgagcggcta
     3301 caagatcaac gtgcagaaga gccaggcctt cctgtacacc aacaaccgcc agaccgagag
     3361 ccagatcatg ggcgagctgc ccttcaccat cgctagcaag cgcatcaagt acctgggcat
     3421 ccagctgacc cgcgacgtga aggacctgtt caaggagaac tacaagcccc tgctgaagga
     3481 gatcaaggag gagaccaaca agtggaagaa catcccctgc agctgggtgg gccgcatcaa
     3541 catcgtgaag atggccatcc tgcccaaggt gatctaccgc ttcaacgcca tccccatcaa
     3601 gctgcccatg accttcttca ccgagctgga gaagaccacc ctgaagttca tctggaacca
     3661 gaagcgcgcc cgcatcgcca agagcatcct gagccagaag aacaaggccg gcggcatcac
     3721 cctgcccgac ttcaagctgt actacaaggc caccgtgacc aagaccgcct ggtactggta
     3781 ccagaaccgc gatatcgacc agtggaaccg caccgagccc agcgagatca tgccccacat
     3841 ctacaactac ctgatcttcg acaagcccga gaagaacaag cagtggggca aggacagcct
     3901 gttcaacaag tggtgctggg agaactggct ggccatctgc cgcaagctga agctggaccc
     3961 cttcctgacc ccctacacca agatcaacag ccgctggatc aaggacctga acgtgaagcc
     4021 caagaccatc aagaccctgg aggagaacct gggcatcacc atccaggaca tcggcgtggg
     4081 caaggacttc atgagcaaga cccccaaggc catggccacc aaggacaaga tcgacaagtg
     4141 ggacctgatc aagctgaaga gcttctgcac cgccaaggag accaccatcc gcgtgaaccg
     4201 ccagcccacc acctgggaga agatcttcgc cacctacagc agcgacaagg gcctgatcag
     4261 ccgcatctac aacgagctga agcagatcta caagaagaag accaacaacc ccatcaagaa
     4321 gtgggccaag gacatgaacc gccacttcag caaggaggac atctacgccg ccaagaagca
     4381 tatgaagaag tgcagcagca gcctggccat ccgcgagatg cagatcaaga ccaccatgcg
     4441 ctaccacctg acccccgtgc gcatggccat catcaagaag agcggcaaca accgctgctg
     4501 gcgcggctgc ggcgagatcg gcaccctgct gcactgctgg tgggactgca agctggtgca
     4561 gcccctgtgg aagagcgtgt ggcgcttcct gcgcgacctg gagctggaga tccccttcga
     4621 ccccgccatc cccctgctgg gcatctaccc caacgagtac aagagctgct gctacaagga
     4681 cacctgcacc cgcatgttca tcgccgccct gttcaccatc gccaagacct ggaaccagcc
     4741 caagtgcccc accatgatcg attggatcaa gaagatgtgg cacatctaca ccatggagta
     4801 ctacgccgcc atcaagaacg acgagttcat cagcttcgtg ggcacctgga tgaagctgga
     4861 gaccatcatc ctgagcaagc tgagccagga gcagaagacc aagcaccgca tcttcagcct
     4921 gatcggcggc aacggcggcg gcagcggcgg cggcagcctg gaagttctgt tccaggggcc
     4981 cgactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga
     5041 tgacaagtag acaatgagat cacatggaca caggaagggg aatatcacac tctggggact
     5101 gtggtggggt cgggggaggg gggagggata gcattgggag atatacctaa tgctagatga
     5161 cacattagtg ggtgcagcgc accagcatgg cacatgtata catatgtaac taacctgcac
     5221 aatgtgcaca tgtaccctaa aacttagagt ataatg
//
