LOCUS       sequence-252454-        9797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MRPS31 R219C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MRPS31-R219C
FEATURES             Location/Qualifiers
     source          1..9797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4637..4661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4669..4687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4712..4731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4713..4731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4737..4757)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4811..4852
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4873..5440
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4890..5442
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5034..5051)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5261..5280
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5454..5456,5457..5459,5460..6173)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5454..5456,5457..5459,5460..6170)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5499..5520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5760..5779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6107..6128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6187..6775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6240..6260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6658..6669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6850..7083
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7202..7220)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7288..7392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7393..7461,7462..8253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7611..7630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8424..9012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8913..8932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9166..9183
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9423..9444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9459..9464,9465..9482,9483..9489)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9497..9513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9502..9524
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9521..9537
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9521..9537
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9587..9606
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg tttcctagag tctcgacgtt cctacctctt
     3481 cgcccccttt cccgccaccc tttgtcctct ggaagcccgg agacatcagc ggctgcgatt
     3541 atgctactca ctgttcggca cggaacagtc aggtaccgca gttcagcgct gttggcccgg
     3601 acaaaaaata acatccaaag atattttggc actaacagtg tgatctgtag caagaaagat
     3661 aagcagtctg ttcgaactga ggagatttcc aaggagactt cagagagcca agacagtgaa
     3721 aaggaaaata cgaaaaaaga cttgttaggc attattaagg gcatgaaagt tgaattaagc
     3781 acagtaaatg tacgaacaac aaagcccccc aaaagaagac cacttaaaag tttggaagct
     3841 gcacttggca ggcttcgaag agctacagaa tatgctccaa agaagagaat tgagcccctg
     3901 agtcctgagt tggtggcagc tgcatctgct gtggcagatt ctctcccttt tgataagcaa
     3961 acaaccaagt cagagctgct gagccagctc cagcagcatg aggaagagtc aagggcacag
     4021 agagatgcaa agcgacctaa aattagtttc agtaacataa tatcagatat gaaagttgcc
     4081 agatctgcta cagctagagt ttgttcaaga ccagagcttc ggattcagtt tgatgaaggc
     4141 tatgacaatt atcctggcca ggagaagacg gatgatctta aaaaaaggaa aaatatattc
     4201 acagggaaaa gacttaatat ttttgacatg atggcagtta ctaaagaagc acctgaaaca
     4261 gacacatcac cttcactttg ggatgtggaa tttgctaagc agttagccac agtaaatgaa
     4321 caaccccttc agaatggatt tgaagagctg atccagtgga caaaagaggg gaaactatgg
     4381 gagttcccaa ttaacaatga agcaggtttt gatgatgatg gttcagaatt tcatgaacat
     4441 atatttctgg agaaacacct ggagagcttt ccaaaacaag gaccaattcg ccacttcatg
     4501 gagctggtga cttgtggcct ttccaaaaac ccatatctta gtgttaaaca gaaggttgaa
     4561 cacatagagt ggtttagaaa ttattttaat gaaaaaaagg atattctaaa agaaagtaac
     4621 atacagttca attagaaccc agctttcttg tacaaagtgg tgatatccaa ttaaccctca
     4681 ctaaagggta gtagtaattt gaatgtttga accctatagt gagtcgtatt accacccaac
     4741 ttttctatac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc
     4801 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     4861 ttagtaatga gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     4921 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     4981 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     5041 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     5101 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     5161 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     5221 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     5281 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     5341 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     5401 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca catatggtga
     5461 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5521 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     5581 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5641 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     5701 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     5761 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     5821 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     5881 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     5941 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     6001 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6061 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6121 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaatcgata
     6181 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6241 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6301 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6361 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6421 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6481 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6541 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6601 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6661 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6721 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     6781 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6841 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6901 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6961 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7021 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7081 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7141 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     7201 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7261 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7381 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7441 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7621 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8221 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9181 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9241 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9301 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9361 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9421 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9481 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
     9541 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9601 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     9661 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     9721 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     9781 actacaaact tagtagt
//
