LOCUS       sequence-252480-       11471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA isolation from keratinocytes via TRAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Lam PY, Harvie EA, Huttenlocher A
  TITLE     Heat shock modulates neutrophil motility in zebrafish.
  JOURNAL   PLoS One. 2013 Dec 19;8(12):e84436. doi: 
            10.1371/journal.pone.0084436. eCollection 2013.
  PUBMED    24367659
REFERENCE   2  (bases 1 to 11471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-19"; volume: "8(12):e84436. doi"; pages: " 
            10.1371/journal.pone.0084436. eCollection 2013"
COMMENT     Sequence Label: Tg(krt4:EGFP-Mmu.Rpl10a)
FEATURES             Location/Qualifiers
     source          1..11471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..12,13..175)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      2766..2775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2772..2774,2775..2777,2778..3488)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2772..2774,2775..2777,2778..3488)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2817..2838)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3078..3097)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3425..3446
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4222..4356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4246..4265)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4300..4319
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6506..6693,6694..6705)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6739..6755)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6739..6755)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6796..6816)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6796..6814)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6835..6851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6835..6851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6848..6870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6859..6875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6883..6889,6890..6907,6908..6913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6928..6949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7066..7083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7237..7825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7317..7336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7996..8787,8788..8856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8619..8638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8857..8961)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8987..9442)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9124..9145)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9336..9355
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9569..9591
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9583..9600
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9584..9600
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9610..9629
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9610..9628
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa
       61 attttactca attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa
      121 agtactccat taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa
      181 actagagatt cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg
      241 ttttatcctg ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa
      301 tgccagtaca cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt
      361 ctcaaaattg ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct
      421 gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct
      481 gcaaatagca ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc
      541 cctcgagctc aagcttcgaa ttcctacagt aaagcttctc cacaatgtcc cggagatcca
      601 gcccggacat gcattttctt gcctttcctt cccttctaag tctgacgtcc ttttaagagc
      661 ttgtgcatga aagcaaattt agagcttatt actcatctta aacacccata caaagtgatg
      721 attgccgtac catgatctca cacctttcac acctggttta tactatgata gttgtagacg
      781 attgcgtaat gctactaaat gcccatcagt gctggctgtg acacccaact gctgccattt
      841 cgtgttgact tgcacgagaa atgagaaatt gtctgactat gcagggtgta ctatgcgtgg
      901 gaacttatca gtggtcatta aatactatgg tttaaagtta gagcaaagtg tgctgtattt
      961 ttgtgtcagc ttagctgctg ttttgtgtgt aaagtaacaa atgacaaata ctcaaactat
     1021 tgaaattaag tagtttttct cagaaattgt aatttactaa gtagtttaaa aatgtgtact
     1081 tttactttcc cttgagcaca tttttagtgc agtattggta cttttatttc actactttcc
     1141 ttcaacctgc agtcactact ttattttttc ttgtctatgt ggattagaca aatcagtcct
     1201 gtgattcctg tccaatcaaa ttgcacagaa ggtaaatcac atcataatga acttcctcaa
     1261 gacatgggcc atttataatt gcagcaaact gtttgccaga attaaaagaa gatgtcaaaa
     1321 atctttacac gcattaaccc agagactgct tagatgcatg tcactgatga gaagatgaca
     1381 gatgttttct gtatgatgac cgaaataacc ttaaacgcac acaagacggc acaagacgtc
     1441 aacatggcta taggtttacg ttgtacccca ttgcaatggg gacgttgtat tttgtttaga
     1501 aatgaaaatt aggttgacgt cagagctcaa cgtcaggtcg atgtcaaggt tcaacatcca
     1561 atctaaaatc atatatcaat gtctaatgat gttacagctt gatgttgtgt ggatgttacc
     1621 cctatgatgt ctatcagaag ttggattgtg gttgccatac ctaatgaata aatgtcatta
     1681 ttttacattt gtttaagatg ttggttcgac attggactta tgtcgctttc caacacaacc
     1741 taaatccacc aaatattaac ttcctatgac atcgttattg gacatcaaaa taacaatatc
     1801 cttagatgct ggctagactt tgaatttagg tcaccacaac ctatatttaa cctaatatta
     1861 acatcttatg atgttgtgtg cctgctgggc aataactaaa tgcactacag aatgttacgt
     1921 ttacacacat gtaaattaca tgtaaatgca ttagcttttt aagacataat actcactact
     1981 aactactctt gagtactttt aaaaaagcta ctttttactc atactttgag taacagttac
     2041 aacagatact tttactcgca ctggattttt aggcatgtaa tggtattttt actatgattt
     2101 ttcagtactc tttccactac tgcagccctc cccatacata atcatatgtt tacacatatg
     2161 gtggagttta gagccataaa ctacattaac tttgttagcc gccagcatta ctgtgcagaa
     2221 ttgtgtgtgt gcacattttc caatatcaat acagaaggaa actctgttcc ctgttccctt
     2281 gtaaatctca acaatgcaac tgttcagctc agggggaaaa atgccctgcc agatccaaac
     2341 gctggcaaaa gtggatggaa aaaagccttt cattaatgta agagttgctg cgcgccccac
     2401 ccagataaaa agagcagagg ttaacatgct ctctacggct gtccagccaa ccagatactg
     2461 aggcagaaac acacccgctt gcagatggtg agagctacac tgtctttttc agagtttcta
     2521 ctggaatgcc tgtcctcaag tctcaagcct ctccttgcat tttctcattc cacctggggc
     2581 aaagccccag gctgggtgtg acaccattta tcttaccact ttctctctgt acctgtctaa
     2641 caggtagggt gtgtgtgaga gtgcgtatgt gtgcaagtgt gtgtgtgaga gcagtcagct
     2701 ccaccccctc aagagtgtgt ataaaattgg tcagccagct gctgagagac acgcagaggg
     2761 atcccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2821 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2881 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2941 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3001 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3061 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3121 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3181 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3241 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3301 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3361 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3421 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3481 tgtacaagtc cggccggact cagatctcga gctcaagctt cgaattcagc agcaaagtct
     3541 cacgcgacac cctgtacgag gcggtgcggg aagtcctgca cgggaaccag cgcaagcgcc
     3601 gcaagtttct ggagacggtg gagctgcaga tcagcctgaa gaactacgac cctcagaagg
     3661 acaaacgttt ctcgggcacc gtcaggctca agtccacccc acgccccaag ttctcggtgt
     3721 gcgttctggg ggaccagcag cactgtgatg aagccaaggc cgtggatatc ccccacatgg
     3781 acatcgaggc gctcaagaag cttaacaaaa acaagaagtt ggtcaagaag ctggctaaga
     3841 agtacgatgc ctttttggcc tctgagtctc tgattaagca gatcccacgt atcctgggcc
     3901 caggcctaaa caaggctggc aagttcccct ccctgctgac acacaatgaa aacatggtgg
     3961 ccaaagtgga tgaggtgaaa tcgacaatca agttccagat gaagaaggtg ctgtgtttgg
     4021 ccgtcgctgt tggccacgtg aagatgaccg atgatgagct agtctacaac attcatctgg
     4081 ctgtcaattt cttggtgtcc ttgcttaaga aaaactggca aaacgtgcgg gctctgtaca
     4141 tcaagagcac catgggcaag ccccagcgtc tgtattaggc ggccgccacc gcggtggagc
     4201 tcgaattaat tcatcgatga tgatccagac atgataagat acattgatga gtttggacaa
     4261 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     4321 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     4381 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     4441 tgtggtatgg ctgattatga tcctctagat cagatctgtc ccgatctttc tcttctgtgc
     4501 tgtcaattta gagtagtttt tgaggtaatt tgggtgcatt ctctgtaaaa acaacaaata
     4561 aatatttata ttgtatgcaa taaatgtaac aattacacat acactctctg attacagcat
     4621 taaagggtta gttcacccaa aaatgaaaag atgtcattaa tgactcaccc tcatgtcgtt
     4681 ccaagcccgt aacacctccg ttcatcttcg gaacacagtt taagatattt tagatttagt
     4741 ccgagagctt tctgtgcctc cattgagaat gtatgtacgg tatactgtcc atgtccagaa
     4801 aggtaataaa aacatcaaag tagtccatgt gacatcagtg ggttagttag aattttttga
     4861 agcatcgaat acattttggt ccaaaaataa caaaacctac gactttattc ggcattgtat
     4921 tctcttccgg gtctgttgtc aatccgcgtt cacgacttcg cagtgacgct acaatgctga
     4981 ataaagtcgt aggttttgtt atttttggac caaaatgtat tttcgatgct tcaaataatt
     5041 ctacctaacc cactgatgtc acatggacta ctttgatgtt tttattacct ttctggacat
     5101 ggacagtata ccgtacatac attttcagtg gagggacaga aagctctcgg actaaatcta
     5161 aaatatctta aactgtgttc cgaagatgaa cggaggtgtt acgggcttgg aacgacatga
     5221 gggtgagtca ttaatgacat tattttcatt tttgggtgaa ctaacccttt aataaaactg
     5281 acatttgcag ccaattgcac actttataat gttaaatact tgttgatttt agaaatacag
     5341 tagcatttag tgacaatcaa acattaattt tatttctgat tatattgaca aaaacacagg
     5401 actgtaaggc tgacatgaca gggtgaattg atttcattac attagttttg atggtaacac
     5461 ttttacaata aggttcgtta gttaactaca ttagttaaca tgaactaata atgaactgca
     5521 cgtatacatg ttaatttcaa catttactaa tactttatat cttgttaaca ttagttaatg
     5581 cactgtgaac taacatgaac aaacaatgaa catctgtatt tctattaact aacgttaaca
     5641 aagattaaca gatacagtaa caaatgtatt gctcatggtt atttaatgtt agttaataca
     5701 tgaactaatc aacctcattg taaagtgtta ccgttttgac aatacaacat gtttggaaaa
     5761 aaatttctcg cttggttttc ttgtgttttg catgaattca aacacaatga gagcacggaa
     5821 tgataatgat tattcaaagc tgcaataggc gccaaatttc ccgtggacct aacgttacca
     5881 attacaaatg cattttagca ttttattacg tgagccttat attgtcaatc gcatgcatta
     5941 gtgaaaatag gcctactaca catataaaag gcgcgccatc aacgtcaaaa catgcacacc
     6001 caaaaaaaaa aaaaaaaaaa aaaaaaaaac tatcagtaaa acaaaatact taatgtactt
     6061 acctcaatat gcttccttag gtttgatggc gaacttttga aggccgatat ttctttatta
     6121 agcgggagac agaggacaca tttcatcttg aatgaatctt tatttacacc acttaaagaa
     6181 aagaattcgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg
     6241 ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt
     6301 ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac
     6361 gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt
     6421 tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt
     6481 ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt
     6541 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
     6601 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
     6661 aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta
     6721 ccttcagcaa gcccagcaga tccactagtt ctagagcggc cggccgccac cgcggtggag
     6781 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     6841 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6901 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6961 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7021 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7081 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7141 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7681 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7861 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7921 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7981 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8041 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8101 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8161 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8221 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8281 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8341 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8401 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8461 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8521 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8581 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8641 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8701 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8761 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8821 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8881 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8941 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     9001 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     9061 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9121 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9181 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9241 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9301 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9361 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9421 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9481 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     9541 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     9601 gagcgcgcgt aatacgactc actatagggc gaattgggta ccaaatagta ggaattaccc
     9661 acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt tgccattttc tactgctatt
     9721 ttaaatcttt tctgtatttg tctgcatttg tctttaccct tgaataaatg ttttagttgt
     9781 tttttttcaa ttatgactgt gtttaacaga cattattaca ataatatgta atagtagtac
     9841 acactattat tatgtaatag tacttgttga ctgtatttga gaactggacc gagtgagtgt
     9901 tacgtcaccc attcaaaatg acttacttct ggctccaaca aaatgaagtt aattcagttg
     9961 ccatttttca ctgtatggac atcgccgtgt tggagctaga cgttgccaat aagcaagaaa
    10021 gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc aacccctcta accaatcaga
    10081 agtaagcttg ttggaagtcc acagcctacc acttgaaagc gggctgcaca aaatctgtca
    10141 aacgttttga acgttggatg tgagagcaca tacttttatt aaggcatctg gttggtcagt
    10201 ttataatatc aacaacttgg gctacagaaa agaaaagtta ttacagaaat tatgattaac
    10261 aagtacatgt taaataaaga ttttaatatg aatgccacca ctggagcatt catgccattt
    10321 ggagcttctt cctgtttgga tcactagaag gaggaggtca ctcattacag ttctcatata
    10381 cagtcgttgg ttggttggtt ggttggtaga ttgattgatt gattgattga ttgattgatt
    10441 gattgattga ttgattgatt gattgattga ttgattggta gtcaaaataa gaaataattt
    10501 ccacagattc attacagaaa tgattaaatg catacataaa aaactngggg ggggggggga
    10561 tacaacaaca cacttaagtc acatttgcct acgtaaagaa aagtaaagaa aatcaatagc
    10621 tatattttac atctcctttt tttgctgtct tttaattagc cttgttttgc tgttatcttg
    10681 attgaaactg taacttcttc acctgcttct tttctttgta ggttttgcca gcccagaaac
    10741 aggcatggct gtgcaaggcc agagcaccat gcacaatgcg ctgcatgtct tcatgaacgg
    10801 ctcaatgtcc tcagtccagg gctcagccaa cgaccccatc ttcctccttc accatgcttt
    10861 cattgacagg taacaaacac gtcatgacat tagactgcac agtttttgac aaagttcata
    10921 caatctgttg tttatagctg ctacaattag tgaagtttgt gaatgtactt ggatgagcag
    10981 cgaaagatca attgagatca attgttagag tttggttgcc ctgcagagca aagaacaaaa
    11041 aataatctgg tggctttact gcgtgaggtt attattggtg gaatagaaac acaaaacata
    11101 attgcattta tttgtttaat tttttatctt atcttaactt tcatcttgca tatttgtttc
    11161 ttacatcatt tctagcatct ttgagcgctg gctaagaact catcagcctc cccggtccat
    11221 ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg tgccattcct
    11281 tcctctttat aggaatggag actacctcct gtccaacaat gctcttggat acgagtacgc
    11341 ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc tgcaaaagac
    11401 gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga ggtttgtcca
    11461 ggagttcttg a
//
