LOCUS       sequence-252482-       12394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVET-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..12394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3298
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3289
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3246..3266
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3346..3463
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3540..3549
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3546..3548,3549..3551,3552..4262)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3546..3548,3549..3551,3552..4262)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3591..3612)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3852..3871)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4199..4220
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4323..4890
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4341..4892
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4485..4502)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4712..4731
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4895..5515
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4968..4987)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             5516..5536
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5639..5833
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    5975..6563
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6028..6048)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6446..6457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(6701..6719,6720..6742,6743..6761,6762..6784,6785..6803,
                     6804..6826,6827..6845,6846..6868,6869..6887,6888..6910,
                     6911..6929,6930..6952,6953..6971)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             7010..7190
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7215..7233)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7216..7233)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7523..7542)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7756..7775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7875..7897
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7935..7953)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8021..8125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8126..8194,8195..8986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8344..8363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9157..9745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9646..9665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9899..9916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9986..10006)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9991..10320
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10171..10306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11454..11519
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11649..11669
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12094..12228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12131..12150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12185..12204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc awtcgatyas krrackrryc tcgacsnnat cgatswcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     2461 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     2521 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     2581 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     2641 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     2701 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     2761 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     2821 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     2881 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     2941 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3001 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3061 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3121 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3181 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3241 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     3301 aatttcgaca tttaaattta attaatctcg acggtatcgg ttaactttta aaagaaaagg
     3361 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     3421 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc acgagactag
     3481 cctcgaggtt taaactacgg gatccaggcc taagcttacg cgtcctagcg ctaccggtcg
     3541 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3601 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3661 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3721 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3781 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3841 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3901 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3961 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4021 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4081 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4141 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4201 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4261 agtccggact cagatctcga ctagctagta gctagctagc tagtcgagct caagcttcga
     4321 attccgcccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     4381 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     4441 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     4501 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     4561 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     4621 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     4681 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     4741 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     4801 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     4861 gtggttttcc tttgaaaaac acgatgataa taccatggct agattagata aaagtaaagt
     4921 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
     4981 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
     5041 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
     5101 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
     5161 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
     5221 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
     5281 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
     5341 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
     5401 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
     5461 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcgccaaa
     5521 aaagaagaga aaggtcgacg gcggtggtgc tttgtctcct cagcactctg ctgtcactca
     5581 aggaagtatc atcaagaaca aggagggcat ggatgctaag tcactaactg cctggtcccg
     5641 gacactggtg accttcaagg atgtatttgt ggacttcacc agggaggagt ggaagctgct
     5701 ggacactgct cagcagatcg tgtacagaaa tgtgatgctg gagaactata agaacctggt
     5761 ttccttgggt tatcagctta ctaagccaga tgtgatcctc cggttggaga agggagaaga
     5821 gccctggctg gtggagagag aaattcacca agagacccat cctgattcag agactgcatt
     5881 tgaaatcaaa tcatcagttt aaggatctcg actagctagt agctagctag ctagtcgagc
     5941 tcaagcttcg aattcccggg actagtcata tgataatcaa cctctggatt acaaaatttg
     6001 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6061 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6121 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6181 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6241 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6301 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6361 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6421 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6481 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6541 ctccctttgg gccgcctccc cgcatcggta cgtatggcca ggtaccttta agaccaatga
     6601 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6661 taattcactc ccaacgaaga caagatctcg agtttaccac tccctatcag tgatagagaa
     6721 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     6781 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     6841 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     6901 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     6961 agtgatagag aaaagtgaaa gtcggatcga tctgcttttt gcttgtactg ggtctctctg
     7021 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7081 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7141 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     7201 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     7261 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     7321 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     7381 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     7441 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     7501 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     7561 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     7621 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     7681 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     7741 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     7801 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     7861 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     7921 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     7981 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8041 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8101 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8161 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8221 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8281 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8341 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8401 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8461 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8521 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8581 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8641 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8701 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8761 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8821 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8881 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8941 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9901 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9961 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    10021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    10081 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    10141 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    10201 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    10261 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10321 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
    10381 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
    10441 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
    10501 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
    10561 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
    10621 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
    10681 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
    10741 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
    10801 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
    10861 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
    10921 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
    10981 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
    11041 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
    11101 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
    11161 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
    11221 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
    11281 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
    11341 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
    11401 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
    11461 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
    11521 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
    11581 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
    11641 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
    11701 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
    11761 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
    11821 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
    11881 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
    11941 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
    12001 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    12061 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    12121 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    12181 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    12241 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    12301 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    12361 tgtatttgtt aaatatgtac tacaaactta gtag
//
