LOCUS       sequence-252490-        6228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control plasmid with only spectinomycin .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Gonzalez LM, Mukhitov N, Voigt CA
  TITLE     Resilient living materials built by printing bacterial spores.
  JOURNAL   Nat Chem Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 
            10.1038/s41589-019-0412-5.
  PUBMED    31792444
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 
            10.1038/s41589-019-0412-5."
COMMENT     Sequence Label: pLG173
FEATURES             Location/Qualifiers
     source          1..6228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1127..1213
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1232..1259
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2034..2052)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2120..2224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2225..2293,2294..3085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2443..2462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3256..3844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3745..3764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaaacaaaa aaattgaaaa aagtgtttcc accatttttt caattttttt ataatttttt
       61 taatctgtta tttaaatagt ttatagttaa atttacattt tcattagtcc attcaatatt
      121 ctctccaaga taactacgaa ctgctaacaa aattctctcc ctatgttcta atggagaaga
      181 ttcagccact gcatttcccg caatatcttt tggtatgatt ttacccgtgt ccatagttaa
      241 aatcatacgg cataaagtta atatagagtt ggtttcatca tcctgataat tatctattaa
      301 ttcctctgac gaatccataa tggctcttct cacatcagaa aatggaatat caggtagtaa
      361 ttcctctaag tcataatttc cgtatattct tttatttttt cgttttgctt ggtaaagcat
      421 tatggttaaa tctgaattta attccttctg aggaatgtat ccttgttcat aaagctcttg
      481 taaccattct ccataaataa attcttgttt gggaggatga ttccacggta ccatttcttg
      541 ctgaataata attgttaatt caatatatcg taagttgctt ttatctccta ttttttttga
      601 aataggtcta attttttgta taagtatttc tttactttga tctgtcaatg gttcagatac
      661 gacgactaaa aagtcaagat cactatttgg ttttagtcca ctctcaactc ctgatccaaa
      721 catgtaagta ccaataaggt tattttttaa atgtttccga agtatttttt tcactttatt
      781 aatttgttcg tatgtattca aatatatcct cctcactatt ttgattagta cctattttat
      841 atccatagtt gttaattaaa taaacttaat ttagtttatt tatagatttc attggcttct
      901 aaatttttta tctagataat aattatttta gttaatttta ttctagatta tatatgatat
      961 gatctttcat ttccataaaa ctaaagtaag tgtaaaccta ttcattgttt taaaaatatc
     1021 tcttgccagt cacgttacgt tattagttat agttattata acatgtattc acgaacgaaa
     1081 atcgccattc gccagctgca ggaattcgac ctctagcttg aggcatcaaa taaaacgaaa
     1141 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     1201 gagtaggaca aatccgccgc tctagctaag cagaaggcca tcctgacgga tggccttttt
     1261 gcgtttctac aaactcttgt taactctaga gctgccataa taaccgggca ggccatgtct
     1321 gcccgtattt cgcgtaagga aatccattat gtactattta attctgcgtg acatcccatc
     1381 gatcagacca gtttttaatt tgtgtgtttc catgtgtcca gtttggaata ctcttaacct
     1441 cattggaaat cgcggcataa tcactggtgg tatgattgat gaccgcgtca acaatgacct
     1501 ttatgccata ttcttcagcg gctgcacaca tttctttaaa ttcttgttca gtacctaagt
     1561 aacggttgcc aatttgatac gatgtcggct gatacagcca gtaccagttc gacatgcttt
     1621 tatctccttg attcccttcc tttacttggt taatcggaga tgtctgaatg gctgtatatc
     1681 ctgcatcatg aatatccttc atattgtgtt ttaacgtatt gaacgaccaa ttccatgcat
     1741 gaagaatggt tccgcttttg atcgacggtg ctgtaagctc attcgatttg ttcgccgttt
     1801 cagcactcgc agccgccggt cctgccagaa ccaaatgaaa cagcaataaa aatccagcga
     1861 ataacggcag taaagaggtt ttgaatcgtt ttgcaaacat tcttgacact ccttatttga
     1921 ttttttgaag acttacttcg gagtcaaaaa tccctcttac ttcattcttc cgcttcctcc
     1981 tttcaaaccg atgtgaagac tggagaattt tgttaattct tgaagacgaa agggcctcgt
     2041 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2101 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2161 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2221 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2281 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2341 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2401 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2461 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2521 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2581 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2641 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2701 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2761 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2821 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2881 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2941 gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3001 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3061 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3121 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3181 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3241 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3301 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3361 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3421 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3481 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3541 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3601 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3661 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3721 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3781 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3841 gaaaaacgcc agcaacgcgg cccgacctcg agctggatac ttcccgtccg ccagggggac
     3901 atgccggcga tgctgaaggt cgcgcgcatt cccgatgaag aggccggtta ccgcctgttt
     3961 gaggatatag taatctttct aaatagcttt ggattggagg agtatggcca ctaatactaa
     4021 gttcagctaa taaaaaaatt tgctaaagaa ctccagctgg atttcactga tgagaatatc
     4081 gtcggagata aatataataa ttccacggac tatagactat actagtatac tccgtctact
     4141 gtacgataca cttccgctca ggtccttgtc ctttaacgag gattgttacg tacgctaatg
     4201 gcgtcaaaac aaagactcta gacctaggcc ttaagatctg atcatatgca tccgcgggcc
     4261 cgggttaacg cgtaatccat ggatcaagag acaggatgag gatcgtttcg catgattgaa
     4321 caagatggat tgcacgcagg ttctccggtg ccctgaatga actgcagaaa gagctggtag
     4381 ttggcgcact gttcgaagaa ctgccgatgt ccagtaagat tcttactatg ctggttgaac
     4441 cggatgctgg taaagctact tgggttgctg cttctactta tggtaccgat acaactactg
     4501 gtgaggaagt taaaggagct cttaaagaaa tccacttcag taggtggccc ggctccatgc
     4561 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa
     4621 cccgttccat gtgctcgccg aggcggtata aatcgccgtg acgatcagcg gtccaatgat
     4681 cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc
     4741 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     4801 aatcataatg gggaaggcca tccagcctcg cgtcgcgact aagaaaatgc cgtcaaatcc
     4861 gctcgccatg acttcactaa cgatgccttt gaaaatcttc aagttctttt ctactaattc
     4921 aaggcgtgtc tcaccaggtt tttggtttgc tccggcgcaa atgcagacaa tatcagcatc
     4981 cttgcagggt atgtttctct ttgatgtctt tttgtttgtg aagtatttca catttatatt
     5041 gtgcaacact tcacaaactt ttgcaagaga aaagttttgt ctgatttatg aacaaaaaag
     5101 aaaccatcat tgatggtttc tttcggtaag tcccgtctag ccttgccctc aatggggaag
     5161 agaaccgctt aagcccgagt cattatataa accatttagc acgtaatcaa agccaggctg
     5221 attctgaccg ggcacttggg cgctgccatt attaaaaatc acttttgcgt tggttgtatc
     5281 cgtgtccgca ggcagcgtca gcgtgtaaat tccgtctgca tttttagtca ttggttttcc
     5341 aggccaagat ccggtcaatt caattactcg gctcccatca tgtttataga tataagcatt
     5401 tacctggctc caatgattcg gattttgata gccgatggtt ttggccgacg ctggatctct
     5461 tttaacaaaa ctgtatttct cggtcctcgt tacaccatca ctgttcgttc cttttaacat
     5521 gatggtgtat gttttgccaa atggatctcc ttttccgatt gtgaattgat ctccatcctt
     5581 aaacgcctgt cgtctggtcc attattgatt tgataaacgg cttttgttgt attcgcatct
     5641 gcacgcaagg taatcgtcag ttgatcattg aaagaatgtg ttacacctgt tttgtaattc
     5701 tcaaggaaaa catgaggcgc ttttgcaata tcatcaggat aaagcacagc tacagacctg
     5761 gcattgatcg tgcctgtcag tttaccatcg ttcacttgaa atgaacccgc tccagcttta
     5821 ttgtcatacc tgccatcagg caattttgtt gccgtattga tagagacaga ggatgaacct
     5881 gcatttgcca gcacaacgcc atgtgagccg cgctgattca taaatatctg gttgtttcca
     5941 ttcgggttcg agagttcctc aggctgtcca gccatcacat tgtgaaatct attgaccgca
     6001 gtgatagcct gatcttcaaa taaagcactc ccgcgatcgc ctatttggct tttccccggg
     6061 aacctcacac catttccgcc tccctcaggt ctggaaaaga aaagaggcgt actgcctgaa
     6121 cgagaagcta tcaccgccca gcctaaacgg attcccccta tgcaagggtt tattgttttc
     6181 taaaatctga ttaccaatta gaatgaatat ttcccaaata ttaaataa
//
