LOCUS       sequence-252492-        3093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5154
FEATURES             Location/Qualifiers
     source          1..3093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             12..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(550..649)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(666..685)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(667..685)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(690..706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(690..706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(808..827)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             819..1628
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(886..905)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1775..2363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2264..2283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2517..2534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2693..2720)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2812..2898)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2947..2969
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2961..2978
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2962..2978
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2994..3093
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccgcggccgc cttgtttaac tttaagaagg agcccttcac catgggaaat atatgtgtcg
       61 gcggatccag gatggcccat caggtgaact ccccagaccg agttagtaac aactcgggtg
      121 acgaagataa cgtaacgtcc agtcaactgc tgagcgtcag acatcaactt gcggagtctg
      181 ctggtctacc aagagatcag catgaatttg ttagtagcca agcacctcaa agcctgagaa
      241 atcgctacaa caatctttac tcacatacgc aaagaacact ggatatggcg gacatgcagc
      301 ataggtacat gacgggagcg tcaggaatca atccgggaat gctgccacat gagaatgtgg
      361 acgatatgcg tagcgctata actgattgga gtgacatgcg cgaagctctg cagcacgcaa
      421 tgggtatcca tgccgacatc ccaccgtctc cagagcgatt tgttgcgact atgaacccga
      481 gcggatcaat tcgaatgtca acactttctc ctagcccgta ccgtaactgg caaaagggtg
      541 ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
      601 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
      661 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
      721 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      781 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      841 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      901 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
      961 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1021 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     1081 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     1141 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1201 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1261 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1321 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     1381 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1441 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     1501 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1561 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     1621 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     1681 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     1741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1921 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2161 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2341 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2401 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2461 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2521 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2581 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2641 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     2701 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     2761 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     2821 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     2881 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     2941 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     3001 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     3061 ttataatgcc aactttgtac aaaaaagcag gct
//
