LOCUS       sequence-252493-        3741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5530
FEATURES             Location/Qualifiers
     source          1..3741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1298..1397)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1414..1433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1415..1433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1438..1454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1438..1454)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1556..1575)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1567..2376
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1634..1653)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2523..3111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3012..3031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3265..3282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3441..3468)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3560..3646)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3695..3717
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3709..3726
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3710..3726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgaacccc attcagtcac gcttctccag tgtgcaagag
      181 ctcagacgat ccaacgttga tattccggcg ctcaaagcca atggccaact ggaggtcgac
      241 ggcaagaggt acgagattcg tgcagccgat gacggaacaa tttcggtcct tcgaccggag
      301 caacaatcca aagcgaaaag ttttttcaag ggcgcttccc agttgatagg tggcagcagc
      361 cagcgcgcgc agattgccca ggcgctcaac gagaaggtcg catcggcacg cactgtcttg
      421 caccagagcg ctatgacggg cggacgcttg gacacccttg agcggggcga aagcagctca
      481 gccacaacag ccatcaaacc cactgccaaa caggctgcgc aaagtacttt taacagcttt
      541 catgagtggg ccaaacaggc agaggcgatg cgaaacccgt ctcgaatgga tatctacaag
      601 atctataaac aagatgcacc tcactcacac cccatgagcg acgagcagca agaagagttc
      661 ctgcacacgc taaaggcatt gaatggcaaa aacggcattg aggtgcgcac tcaggaccac
      721 gacagcgtca gaaataaaaa agaccgcaac ctggacaagt acatcgcaga gagcccggat
      781 gcaaagaggt ttttctatcg aattatcccc aaacatgagc gccgagaaga taagaatcaa
      841 gggcgattga ccattggcgt gcaaccccaa tatgcaacac agttgacccg cgccatggca
      901 accctgatag ggaaggaaag tgcaatcacg catggcaaag taataggccc cgcctgccac
      961 ggccaaatga ccgattcggc agttttgtat atcaacggtg atgttgcaaa ggcagaaaag
     1021 ctgggcgaga aactgaaaca gatgagcggc attcctctgg atgcgttcgt tgagcacacc
     1081 cctttgagca tgcaatccct gagtaaaggt ctgtcctatg cagaaagcat cctgggcgac
     1141 accagaggcc atgggatgtc gcgagcggaa gtgatcagcg atgccttgag gatggacggg
     1201 atgccatttc tggccagatt gaagctatca ctgtctgcca atggctatga cccggacaac
     1261 ccggcccttc gaaacacgaa aaagggtggg cgcgccgacc cagctttctt gtacaaagtt
     1321 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1381 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1441 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1501 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1561 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1621 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1681 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1741 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1801 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1861 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1921 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1981 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2041 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2101 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2161 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2221 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2281 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2341 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2401 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2461 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2641 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3301 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3361 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3421 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3481 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3541 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3601 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3661 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3721 gccagtctta agctcgggcc c
//
