LOCUS       sequence-252494-        4002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5617
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1448..1547)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1564..1583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1565..1583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1588..1604)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1588..1604)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1706..1725)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1717..2526
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1784..1803)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2673..3261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3162..3181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3415..3432
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3591..3618)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3710..3796)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3845..3867
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3859..3876
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3860..3876
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3892..3991
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgagacccg tcggtggacc ggctccaggc
       61 tattatccgc caacctatga agctgagcgt cccactgcgc aagctgcagg aaacgatcgc
      121 gcccgatctt cacaggccag ttcctctcca gcagccagcg ttgcgccaga gactccaatg
      181 ctgggggacc tgaagcgctt tccagccggg cgctatccgg atatgaaggt agaaaatatc
      241 cggctgaaaa tcgaggggca ggagcctggc ggaaaggatg gcgtaaagca caccagaagg
      301 cgtaagccgg acgcagcagg cagcagtcat gtgcacggcg gccagagcgt ggcctcgacc
      361 tcggcttcag ctcaaagcaa agcattgcag gatacgaact tcaaggcgag cgatcttgcc
      421 gagctcgcgc gctggtgtga gagcccgcac ccctatgcgc tggcaccctc aaaagcagcg
      481 gggaaaagca gccaactgtc tgcaaatgtt gtgagcatcc tgttgcaaga aggcaagcac
      541 gcccttgaac agcgccttga ggctcaaggt ctcaagctgg ccgacgttgt tgtctcggaa
      601 ggtcgggacc accttcatat aaatctcaat taccttgaaa tggacagttg tctggggacg
      661 tccaagggtt tatgggcacc tgacagtaat gacaagaaac tgattgccaa ggcagcgcgt
      721 tattttgatg atttcaacgc gcaaaagtta cctgagctgg cgccgttgac gaagatgaaa
      781 agcaaggaca gtctcggtgt catgcgcgag ctgttacgtg atgcgccggg gcttgttatt
      841 ggtgagggtc acaattcaac gtccagcaag cgtgaactga tcaataacat gaagagcttg
      901 aaggccagtg gcgtgaccac gctttttatg gagcacctct gcgccgagtc acatgacaag
      961 gcgctcaata attacctgag cgcgcccaaa ggcagtccga tgcctgccag gctgaaaaac
     1021 tacctcgatt tgcagagtca gggtcatcag gccccggaag agctccacac gaaatataac
     1081 ttcaccacct tggtggaagc ggccaagcac gccgggttgc gcgttgtctc gctggataca
     1141 acgtccacct atatggcccc ggagaaagct gagataaagc gtgcccaagc catgaattac
     1201 tacgcagcag aaaaaataag gctgagcaaa ccggaaggta agtgggtcgc ttttgtcggg
     1261 gcaacgcacg ccacttcctg tgacggagtc ccagggttgg cagagttgca tggggtacgc
     1321 agtctggtga tcgatgatct gggcctcaag tcccgagcga ccgtcgatat caatgtgaaa
     1381 aactacggcg gcaagctgaa tccagacgtg aggctttcct ataaggtctg caagggtggg
     1441 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1501 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1561 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1621 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1681 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1741 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1801 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1861 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1921 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1981 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2041 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2101 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2161 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2221 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2281 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2341 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2401 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2461 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2521 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2581 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2641 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2701 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2761 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2821 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2881 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3181 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3241 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3301 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3361 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3421 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3481 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3541 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3601 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3661 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3721 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3781 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3841 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg
     3901 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     3961 ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg cc
//
