LOCUS       sequence-252496-        8577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fusion protein of Gaussia luciferase, Channelrhodopsin-2, and 
            enhanced yellow fluorescent protein (luminopsin) for bioluminescent 
            optogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Berglund K, Birkner E, Augustine GJ, Hochgeschwender U
  TITLE     Light-emitting channelrhodopsins for combined optogenetic and 
            chemical-genetic control of neurons.
  JOURNAL   PLoS One. 2013;8(3):e59759. doi: 10.1371/journal.pone.0059759. Epub 
            2013 Mar 27.
  PUBMED    23544095
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(3):e59759. doi"; pages: " 
            10.1371/journal.pone.0059759. Epub 2013 Mar 27"
COMMENT     Sequence Label: pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)
FEATURES             Location/Qualifiers
     source          1..8577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        98..401
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        403..678
                     /label=chicken beta-actin promoter
     intron          679..1695
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1619..1641
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1703..1722
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1784..1793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1790..2344
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     regulatory      2384..2393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2390..3316
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3329..3331,3332..4045)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3371..3392)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3632..3651)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3979..4000
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4075..4116
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4126..4143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4166..4183)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4172..4396
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4442..4870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4529..4548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4739..4760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4879..4899)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4884..5213
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5064..5199
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5126..5145
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5280..6074
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5334..5353)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5944..5963
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6250..6371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6287..6306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6341..6360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6420..6436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6420..6436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6433..6455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6444..6460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6468..6474,6475..6492,6493..6498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6513..6534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6651..6668)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6822..7410)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6902..6921)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7581..8372,8373..8441))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8204..8223
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8442..8546)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctggc gcgccggtac catgcattag ttattaatag taatcaatta
       61 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      181 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      241 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      301 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      361 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
      421 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
      481 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg
      541 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
      601 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      661 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
      721 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
      781 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
      841 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
      901 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
      961 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     1021 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     1081 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     1141 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     1201 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     1261 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     1321 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     1381 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
     1441 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     1501 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     1561 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     1621 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     1681 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     1741 aaagaagcta gcgctaccgg actcagatct cgagctcaag cttgccacca tgggagtcaa
     1801 agttctgttt gccctgatct gcatcgctgt ggccgaggcc aagcccaccg agaacaacga
     1861 agacttcaac atcgtggccg tggccagcaa cttcgcgacc acggatctcg atgctgaccg
     1921 cgggaagttg cccggcaaga agctgccgct ggaggtgctc aaagagatgg aagccaatgc
     1981 ccggaaagct ggctgcacca ggggctgtct gatctgcctg tcccacatca agtgcacgcc
     2041 caagatgaag aagttcatcc caggacgctg ccacacctac gaaggcgaca aagagtccgc
     2101 acagggcggc ataggcgagg cgatcgtcga cattcctgag attcctgggt tcaaggactt
     2161 ggagcccatg gagcagttca tcgcacaggt cgatctgtgt gtggactgca caactggctg
     2221 cctcaaaggg cttgccaacg tgcagtgttc tgacctgctc aagaagtggc tgccgcaacg
     2281 ctgtgcgacc tttgccagca agatccaggg ccaggtggac aagatcaagg gggccggtgg
     2341 tgacggggat ccactagtcc agtgtggtgg aattgccctt gctgccacca tggactatgg
     2401 cggcgctttg tctgccgtcg gacgcgaact tttgttcgtt actaatcctg tggtggtgaa
     2461 cgggtccgtc ctggtccctg aggatcaatg ttactgtgcc ggatggattg aatctcgcgg
     2521 cacgaacggc gctcagaccg cgtcaaatgt cctgcagtgg cttgcagcag gattcagcat
     2581 tttgctgctg atgttctatg cctaccaaac ctggaaatct acatgcggct gggaggagat
     2641 ctatgtgtgc gccattgaaa tggttaaggt gattctcgag ttcttttttg agtttaagaa
     2701 tccctctatg ctctaccttg ccacaggaca ccgggtgcag tggctgcgct atgcagagtg
     2761 gctgctcact tgtcctgtca tccttatccg cctgagcaac ctcaccggcc tgagcaacga
     2821 ctacagcagg agaaccatgg gactccttgt ctcagacatc gggactatcg tgtggggggc
     2881 taccagcgcc atggcaaccg gctatgttaa agtcatcttc ttttgtcttg gattgtgcta
     2941 tggcgcgaac acattttttc acgccgccaa agcatatatc gagggttatc atactgtgcc
     3001 aaagggtcgg tgccgccagg tcgtgaccgg catggcatgg ctgtttttcg tgagctgggg
     3061 tatgttccca attctcttca ttttggggcc cgaaggtttt ggcgtcctga gcgtctatgg
     3121 ctccaccgta ggtcacacga ttattgatct gatgagtaaa aattgttggg ggttgttggg
     3181 acactacctg cgcgtcctga tccacgagca catattgatt cacggagata tccgcaaaac
     3241 caccaaactg aacatcggcg gaacggagat cgaggtcgag actctcgtcg aagacgaagc
     3301 cgaggccgga gccgtgccag cggccgccgt gagcaagggc gaggagctgt tcaccggggt
     3361 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3421 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3481 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     3541 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3601 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3661 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3721 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3781 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3841 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3901 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     3961 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4021 cggcatggac gagctgtaca agtaacatat gctctagagg gcccgcggtt cgaaggtaag
     4081 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     4141 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     4201 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4261 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4321 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4381 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     4441 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4501 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4561 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4621 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4681 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4741 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4801 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4861 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4921 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4981 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5041 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5101 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     5161 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     5221 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     5281 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5341 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5401 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5461 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5521 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5581 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5641 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5701 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5761 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5821 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5881 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5941 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     6001 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6061 acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac
     6121 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6181 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6241 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6301 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6361 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     6421 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6481 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6541 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6601 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6661 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6721 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6781 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6841 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6901 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6961 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7021 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7081 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7141 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7201 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7261 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7321 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7381 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7441 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7501 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7561 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7621 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7681 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7741 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7801 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7861 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7921 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7981 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8041 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8101 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8161 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8221 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8281 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8341 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8401 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8461 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8521 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
