LOCUS       sequence-252506-       12135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pEF1a driving expression of nuclear iRFP713, and mu6 
            expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus 
            destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0464
FEATURES             Location/Qualifiers
     source          1..12135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    109..697
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(162..182)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(580..591)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(700..716)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(701..717)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             807..1040
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1065..1083)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1066..1083)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1373..1392)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1606..1625)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1725..1747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1785..1803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(1982..2570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2062..2081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2784..2803)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2795..3610
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2795..3610
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2868..2887)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3785..3802
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3872..3892)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3877..4206
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4057..4192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5340..5405
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5535..5555
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5980..6114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6017..6036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6071..6090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6282..6915
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6962..7087
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7584..7817
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8002..8046
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8331..8448
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(8727..8760)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(9188..9221)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(9649..9682)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9928..10139
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          10140..11078
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11035..11055
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             11101..11121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttacagaga tgttacgaac cactagtgca ctgcagtaca tgactgactg aggccgcgac
       61 tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
      121 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
      181 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
      241 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
      301 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
      361 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
      421 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
      481 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
      541 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
      601 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
      661 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg
      721 aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
      781 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
      841 tctgcttttt gcttgtactg ggtttctctg gttagaccag atctgagcct gggagctctc
      901 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      961 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     1021 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     1081 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     1141 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     1201 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     1261 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     1321 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     1381 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     1441 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     1501 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     1561 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
     1621 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     1681 ttgtctgctc ccggcatccg cttacagaca agctgtgacc atctccggga gctgcatgtg
     1741 tcagaggttt tcaccgtcat caccgaaacg cgcgagatga aagggcctcg tgatacgcct
     1801 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1861 gggaaatgtg ccgagcggcc gcacggttat ccacagaatc aggggataac gcaggaaaga
     1921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2401 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2641 tcatgactga tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc
     2701 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     2761 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     2821 ttgctccagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2881 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
     2941 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     3001 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     3061 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt
     3121 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     3181 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgcct
     3241 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3301 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
     3361 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3421 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3481 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3541 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3601 gtttttctaa tcagaattgg ttaattggtt gtaacacgcg gccgccaatt ttttacggtt
     3661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3721 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3781 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3841 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     3901 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3961 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4021 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4081 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4141 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4201 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     4261 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     4321 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     4381 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     4441 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     4501 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     4561 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     4621 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     4681 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     4741 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     4801 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     4861 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     4921 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     4981 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     5041 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     5101 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     5161 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     5221 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     5281 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     5341 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     5401 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     5461 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     5521 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     5581 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     5641 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     5701 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     5761 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     5821 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     5881 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     5941 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     6001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6061 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     6121 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     6181 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     6241 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     6301 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6361 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     6421 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     6481 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     6541 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     6601 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     6661 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     6721 tttttgcctg tactgggact ctctggttag accagatctg agcctgggag ctctctggct
     6781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6901 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6961 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7021 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7081 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7141 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7201 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7261 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7321 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7381 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     7441 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7501 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7561 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7621 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7681 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     7741 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     7801 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     7861 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     7921 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     7981 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     8041 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     8101 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     8161 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     8221 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     8281 ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga
     8341 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     8401 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     8461 agggacagca gagatccagt ttggccctga attcgcatct agactgaact ggccgataat
     8521 tgcagacgaa cggatccgac gcgccatctc taggcccgcg ccggccccct cgcacggact
     8581 tgtgggagaa gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta
     8641 actatagagg cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac
     8701 attttacatg ataggcttgg atttctataa cttcgtatag catacattat acgaagttat
     8761 aaacagcaca aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa
     8821 gtatcccttg gagaaccacc ttgtttgacg gaggctaagc gtcgcaagtt taagagctaa
     8881 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
     8941 accgagtcgg tgcttttttt gctgccaaac cagatgtcaa cacagctaca acggatccga
     9001 cgcgccatct ctaggcccgc gccggccccc tcgcacggac ttgtgggaga agctcggcta
     9061 ctcccctgcc ccggttaatt tgcatataat atttcctagt aactatagag gcttaatgtg
     9121 cgataaaaga cagataatct gttcttttta atactagcta cattttacat gataggcttg
     9181 gatttctata acttcgtata gcatacatta tacgaagtta taaacagcac aaaaggaaac
     9241 tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata agtatccctt ggagaaccac
     9301 cttgtttgac ggaggctaag cgtcgcaagt ttaagagcta agctggaaac agcatagcaa
     9361 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     9421 tgctggatgc acacactggc ttaagatgac aacggatccg acgcgccatc tctaggcccg
     9481 cgccggcccc ctcgcacgga cttgtgggag aagctcggct actcccctgc cccggttaat
     9541 ttgcatataa tatttcctag taactataga ggcttaatgt gcgataaaag acagataatc
     9601 tgttcttttt aatactagct acattttaca tgataggctt ggatttctat aacttcgtat
     9661 agcatacatt atacgaagtt ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta
     9721 attgtgtgtt ttgagactat aagtatccct tggagaacca ccttgtttga cggaggctaa
     9781 gcgtcgcaag tttaagagct aagctggaaa cagcatagca agtttaaata aggctagtcc
     9841 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgctggttc tgtatgggca
     9901 cagacaacct aaacggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     9961 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
    10021 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
    10081 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
    10141 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
    10201 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
    10261 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
    10321 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
    10381 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
    10441 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
    10501 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
    10561 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
    10621 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
    10681 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
    10741 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
    10801 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
    10861 acctcgatta gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
    10921 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
    10981 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
    11041 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agccactatg
    11101 cccaagaaga agaggaaggt ggaggacgcc ggttctgctg aaggttctgt tgctagacaa
    11161 ccagacttgt tgacttgtga cgacgaacca atccacatcc caggtgctat ccaaccacac
    11221 ggtttgttgt tggctttggc tgctgacatg actatcgttg ctggttctga caacttgcca
    11281 gaattgactg gtttggctat cggtgctttg atcggtagat ctgctgctga cgttttcgac
    11341 tctgaaactc acaacagatt gactatcgct ttggctgaac caggtgctgc tgttggtgct
    11401 ccaatcactg ttggtttcac tatgagaaag gacgctggtt tcatcggttc ttggcacaga
    11461 cacgaccaat tgatcttctt ggaattggaa ccaccacaaa gagatgttgc tgaaccacaa
    11521 gctttcttca gaagaactaa ctctgctatc agaagattgc aagctgctga aactttggaa
    11581 tctgcttgtg ctgctgctgc tcaagaagtt agaaagatca ctggtttcga cagagttatg
    11641 atctacagat tcgcttctga cttctctggt gaagttatcg ctgaagacag atgtgctgaa
    11701 gttgaatcta agttgggttt gcactaccca gcttctactg ttccagctca agctagaaga
    11761 ttgtacacta tcaacccagt tagaatcatc ccagacatca actacagacc agttccagtt
    11821 actccagact tgaacccagt tactggtaga ccaatcgact tgtctttcgc tatcttgaga
    11881 tctgtttctc cagttcactt ggaattcatg agaaacatcg gtatgcacgg tactatgtct
    11941 atctctatct tgagaggtga aagattgtgg ggtttgatcg tttgtcacca cagaactcca
    12001 tactacgttg acttggacgg tagacaagct tgtgaattgg ttgctcaagt tttggcttgg
    12061 caaatcggtg ttatggaaga aggatcctaa actagagatc tatgtgagga tcctaactcg
    12121 agatcgagct gagca
//
