LOCUS       sequence-252507-       12135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pEF1a driving expression of nuclear iRFP713, and mu6 
            expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus 
            destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0465
FEATURES             Location/Qualifiers
     source          1..12135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(244..277)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(705..738)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(1166..1199)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1445..1656
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1657..2595
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2552..2572
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2618..2638
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3761..4349
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3814..3834)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4232..4243)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4352..4368)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4353..4369)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4459..4692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4717..4735)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4718..4735)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5025..5044)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5258..5277)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5377..5399
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5437..5455)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(5634..6222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5714..5733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6436..6455)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6447..7262
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6447..7262
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6520..6539)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7437..7454
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7524..7544)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7529..7858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7709..7844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8992..9057
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9187..9207
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9632..9766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9669..9688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9723..9742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9934..10567
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10614..10739
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11236..11469
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11654..11698
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11983..12100
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gccctgaatt cgcatctaga ctgaactggc cgataattgc agacgaacgg atccgacgcg
       61 ccatctctag gcccgcgccg gccccctcgc acggacttgt gggagaagct cggctactcc
      121 cctgccccgg ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat
      181 aaaagacaga taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt
      241 tctataactt cgtatagcat acattatacg aagttataaa cagcacaaaa ggaaactcac
      301 cctaactgta aagtaattgt gtgttttgag actataagta tcccttggag aaccaccttg
      361 tttgacggag gctaagcgtc gcaagtttaa gagctaagct ggaaacagca tagcaagttt
      421 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgct
      481 gccaaaccag atgtcaacac agctacaacg gatccgacgc gccatctcta ggcccgcgcc
      541 ggccccctcg cacggacttg tgggagaagc tcggctactc ccctgccccg gttaatttgc
      601 atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag ataatctgtt
      661 ctttttaata ctagctacat tttacatgat aggcttggat ttctataact tcgtatagca
      721 tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt aaagtaattg
      781 tgtgttttga gactataagt atcccttgga gaaccacctt gtttgccaca acgaagatta
      841 taccggttta agagctaagc tggaaacagc atagcaagtt taaataaggc tagtccgtta
      901 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgc tggatgcaca cactggctta
      961 agatgacaac ggatccgacg cgccatctct aggcccgcgc cggccccctc gcacggactt
     1021 gtgggagaag ctcggctact cccctgcccc ggttaatttg catataatat ttcctagtaa
     1081 ctatagaggc ttaatgtgcg ataaaagaca gataatctgt tctttttaat actagctaca
     1141 ttttacatga taggcttgga tttctataac ttcgtatagc atacattata cgaagttata
     1201 aacagcacaa aaggaaactc accctaactg taaagtaatt gtgtgttttg agactataag
     1261 tatcccttgg agaaccacct tgtttgacgg aggctaagcg tcgcaagttt aagagctaag
     1321 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
     1381 ccgagtcggt gctttttttg ctggttctgt atgggcacag acaacctaaa cggtgcccgt
     1441 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     1501 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     1561 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     1621 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     1681 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     1741 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     1801 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     1861 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     1921 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     1981 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     2041 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     2101 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     2161 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     2221 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     2281 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     2341 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgcgcttt
     2401 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     2461 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     2521 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     2581 tttcttccat ttcaggtgtc gtgagctagc cactatgccc aagaagaaga ggaaggtgga
     2641 ggacgccggt tctgctgaag gttctgttgc tagacaacca gacttgttga cttgtgacga
     2701 cgaaccaatc cacatcccag gtgctatcca accacacggt ttgttgttgg ctttggctgc
     2761 tgacatgact atcgttgctg gttctgacaa cttgccagaa ttgactggtt tggctatcgg
     2821 tgctttgatc ggtagatctg ctgctgacgt tttcgactct gaaactcaca acagattgac
     2881 tatcgctttg gctgaaccag gtgctgctgt tggtgctcca atcactgttg gtttcactat
     2941 gagaaaggac gctggtttca tcggttcttg gcacagacac gaccaattga tcttcttgga
     3001 attggaacca ccacaaagag atgttgctga accacaagct ttcttcagaa gaactaactc
     3061 tgctatcaga agattgcaag ctgctgaaac tttggaatct gcttgtgctg ctgctgctca
     3121 agaagttaga aagatcactg gtttcgacag agttatgatc tacagattcg cttctgactt
     3181 ctctggtgaa gttatcgctg aagacagatg tgctgaagtt gaatctaagt tgggtttgca
     3241 ctacccagct tctactgttc cagctcaagc tagaagattg tacactatca acccagttag
     3301 aatcatccca gacatcaact acagaccagt tccagttact ccagacttga acccagttac
     3361 tggtagacca atcgacttgt ctttcgctat cttgagatct gtttctccag ttcacttgga
     3421 attcatgaga aacatcggta tgcacggtac tatgtctatc tctatcttga gaggtgaaag
     3481 attgtggggt ttgatcgttt gtcaccacag aactccatac tacgttgact tggacggtag
     3541 acaagcttgt gaattggttg ctcaagtttt ggcttggcaa atcggtgtta tggaagaagg
     3601 atcctaaact agagatctat gtgaggatcc taactcgaga tcgagctgag cagttacaga
     3661 gatgttacga accactagtg cactgcagta catgactgac tgaggccgcg actctagagt
     3721 cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa
     3781 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3841 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3901 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3961 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4021 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4081 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4141 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4201 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4261 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4321 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt
     4381 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4441 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     4501 ttgcttgtac tgggtttctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4561 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4621 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4681 aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat
     4741 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
     4801 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
     4861 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
     4921 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
     4981 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     5041 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     5101 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     5161 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     5221 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     5281 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5341 tcccggcatc cgcttacaga caagctgtga ccatctccgg gagctgcatg tgtcagaggt
     5401 tttcaccgtc atcaccgaaa cgcgcgagat gaaagggcct cgtgatacgc ctatttttat
     5461 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5521 tgccgagcgg ccgcacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgact
     6301 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
     6361 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     6421 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcca
     6481 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     6541 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     6601 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     6661 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     6721 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
     6781 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     6841 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc ctcgctcagg
     6901 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     6961 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     7021 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     7081 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     7141 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     7201 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     7261 aatcagaatt ggttaattgg ttgtaacacg cggccgccaa ttttttacgg ttcctggcct
     7321 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7381 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7441 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7501 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7681 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7741 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7861 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     7921 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     7981 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     8041 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     8101 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     8161 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     8221 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     8281 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     8341 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     8401 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     8461 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     8521 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     8581 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     8641 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     8701 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     8761 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     8821 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     8881 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     8941 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     9001 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     9061 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     9121 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     9181 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     9241 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     9301 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     9361 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     9421 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     9481 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     9541 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     9601 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     9661 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9721 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     9781 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     9841 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     9901 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     9961 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
    10021 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
    10081 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
    10141 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
    10201 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
    10261 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
    10321 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
    10381 tgtactggga ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10441 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10501 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10561 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10621 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    10681 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10741 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10801 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10861 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10921 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10981 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    11041 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
    11101 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    11161 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    11221 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    11281 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
    11341 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    11401 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    11461 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    11521 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    11581 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    11641 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    11701 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    11761 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    11821 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    11881 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    11941 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
    12001 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    12061 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
    12121 cagagatcca gtttg
//
