LOCUS       sequence-252539-        3453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5539
FEATURES             Location/Qualifiers
     source          1..3453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             12..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(910..1009)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1026..1045)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1027..1045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1050..1066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1050..1066)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1168..1187)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1179..1988
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1246..1265)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2135..2723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2624..2643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2877..2894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3053..3080)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3172..3258)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3307..3329
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3321..3338
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3322..3338
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3354..3453
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccgcggccgc cttgtttaac tttaagaagg agcccttcac catggggcta tgtatttcaa
       61 aacactctgg tagcagttac agctacagtg atagcgaccg ctggcaagtg cctgcatgcc
      121 ctccaaacgc caggtctgta tccagtcatc aaacagcatc tgcgagtgac atcgcatcag
      181 gcgatgtgga tgaacgtcct gcaacgtttt ctcattttca acttgcgcgg tgcggtggag
      241 agtacacgct tagcatggtt tctgcagcgg cttatcaagc agaaagacgg catcgcggta
      301 atttaataaa agatcgtagt caatccatac tcccatgggt ccaggtatat cattctaaaa
      361 aaggtttgga ttacagcttc cagatcgaca gaactacgac tgttaaagtg gctggattca
      421 actgctctat ccccaataac agagggactc ggcatttata cagcgctggt acgagtcaga
      481 caaacatgcc tgtcatcgca gacaacatga gcgcatgcat tgctgtcgcg tgtgcggcgg
      541 aaaacgtgga tgctggcacg ggtgaacgta ggccgggggc gaaagttcgc gtattccatc
      601 tactcccttt tcgacgcgaa gaccttgtgc cagaagaagt tttagcttct gtgcgcgatt
      661 atctgcgaac gaccaaagaa caggggctaa caatgcgcgt agctatgcat ggagggaata
      721 cagagggtga tttctcagtc agcactgcgc aggcattgaa aggcctgttt gctaatgaag
      781 ggatcccgct tgaatttgac gagacctgtg caaaccgaac gtctgaaaca ctgcttggtg
      841 ccgttatctt agatgacaac tcgactcatt tcataaaaca tctggtcgca caaaagggtg
      901 ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
      961 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     1021 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     1081 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     1141 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     1201 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     1261 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     1321 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1381 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     1441 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     1501 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1561 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1621 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1681 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     1741 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1801 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     1861 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1921 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     1981 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     2041 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2281 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2881 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2941 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3001 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3061 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3121 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     3181 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     3241 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     3301 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     3361 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     3421 ttataatgcc aactttgtac aaaaaagcag gct
//
