LOCUS       sequence-252540-        3753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP5534
FEATURES             Location/Qualifiers
     source          1..3753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1199..1298)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1315..1334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1316..1334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1339..1355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1339..1355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1457..1476)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1468..2277
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1535..1554)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2424..3012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2913..2932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3166..3183
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3342..3369)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3461..3547)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3596..3618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3610..3627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3611..3627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3643..3742
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtttaact ttaagaagga gcccttcacc atgaaaatac ataacgctgg cctaacccca
       61 cctttgccgg gcatttcgaa tggaaacgtt ggaaaggcgg cgcaatcatc aataactcaa
      121 ccgcagagcc agcaaggctc ttatggcttg ccaccagaaa gctctgagac tcgccctgat
      181 agggcgcgtg cgaactatcc atattcatca gtacaaacac ggttgccgcc cgttgcgtct
      241 gctgggaaac cgctgcctga tacaccatct tctttgcccg gctacttact gttgcgaagg
      301 ctggaccatc gccctgtgga tcaggaaggt accaaaagtc tgatcccggc agacaaggct
      361 gtggctgaag cgcgccgtgc attgcccttt ggaagaggca atattgatgt ggatgcgcaa
      421 ctttccaatc tggaaagtgg agcccgcacc cttgcagcaa ggtgcttgag aaaagatgcc
      481 gaggccgccg gtcatgagcc tatgcctgcg aatgagccga tgaactggca tgttcttgtt
      541 gcgatgtcag gccaggtgtt cggcgcgggc aactgtggcg aacatgctcg tatagcgagc
      601 ttcgcctatg gagctttggc ccaggaaaac ggacgatctg aatatgaaaa catctacttg
      661 gctgcatcga ctgaggaaga tcatgtgtgg gctgaaaccg acgaatccca gtctggcacc
      721 tcaacgattg tcatggatcc gtggtcaaat ggttcagcca tatttgcgga ggacagtagg
      781 tttgcgaaaa atcgaaatgc tgtagagcgt acggatacgt ttaatctttc aaccgcagcc
      841 gaagcgggca aaattacgcg tgagacagcc gagaaggctt tgacgcaggt cacaacccga
      901 ttgcagaaac gcctggcgga tcagcaggag caagtctcgc ccatcaaaag tggtcgctat
      961 cgaccagaaa aatcggtact tgatgatgca tttgtccgca gagtgagcga caagttgacc
     1021 tcccctgatt tgcggcgtgc actacaggta gatattgaag cggtcggagt cgcaatgtcg
     1081 ctcggcacca agggcgtcaa ggacgctact cgacaagccc gacctttggt tgagcttgca
     1141 gtgaaggtcg cctctcctca aggcttggcg agacgagatg tcaagggtgg gcgcgccgac
     1201 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1261 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1321 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1381 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1441 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1501 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1561 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1621 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1681 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1741 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1801 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1861 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1921 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1981 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2041 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2101 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2161 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2221 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2281 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2341 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3181 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3241 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3301 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3361 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3421 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3481 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3541 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3601 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
     3661 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
     3721 actttgtaca aaaaagcagg ctccgcggcc gcc
//
