LOCUS       sequence-252541-        3735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytopathogen Pseudomonas syringae pv. tomato type III secretion 
            system effector (gene lacking stop codon) Gateway donor clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Munkvold KR, Martin ME, Bronstein PA, Collmer A
  TITLE     A survey of the Pseudomonas syringae pv. tomato DC3000 type III 
            secretion system effector repertoire reveals several effectors that 
            are deleterious when expressed in Saccharomyces cerevisiae.
  JOURNAL   Mol Plant Microbe Interact. 2008 Apr;21(4):490-502. doi: 
            10.1094/MPMI-21-4-0490.
  PUBMED    18321194
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Plant Microbe Interact"; date: "2008-04"; volume: "21"; issue: 
            "4"; pages: "490-502"
COMMENT     Sequence Label: pCPP3416
FEATURES             Location/Qualifiers
     source          1..3735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             144..164
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1324..1423)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1440..1459)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1441..1459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1464..1480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1464..1480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1582..1601)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1593..2402
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1660..1679)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2549..3137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3038..3057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3291..3308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3467..3494)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3586..3672)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3721..3735,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3735,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgctttct tataatgcca actttgtaca
      121 aaaaagcagg ctccgcggcc gccttgttta actttaagaa ggagcccttc accatgaaaa
      181 cagtcagcaa tcactcgata cctagtacaa atctcgtcgt ggatgcggga acggaaactt
      241 cggcgcagaa atcccagccg gtttgcagcg aaatccagcg taacagcaag atcgaaaaag
      301 cagtcatcga acacattgcc gaccacccgg cagcgaaaat gacaataagc gcgctggttg
      361 acacgttgac agacgttttt gtcagggctc atggggaggt taaggggtgg gccgaaatcg
      421 tccaggcagt ctctcgccct catgacagta atcgacacgg cagtggagtg ctcagcccgc
      481 gctttgatgt aatggggagt gttggttgga atgcggcagc tatccgggcc accagtcgcg
      541 tcgggacgct tcgagagaaa ggtacactgt tcactaacct tatgctcagt aacaacttta
      601 aacatttgct taaacgagtg gttaacgatc cagccttgca gcaaaagctc gacggtgggt
      661 tagacctcaa ctatctgaag gcttgtgaag gcgatcttta tgtcatgtca gggtgggctg
      721 cacgggctag cgaaagtcgt gaacaaattg gcaaagcccg gtatgaaacg gcatcaaatc
      781 ttagccagac gctgatcagt gcacgtgagt tggcttttca tcgtcacaat ccggttaatc
      841 atccgtctgc ccaaacgaaa gtgggcttcg ataagggttt gcctgaggaa tctgatctgc
      901 aggttctgag aggccatggc agcagtgtat ggagtgtaaa accgggcagc gatttcgcaa
      961 agcgtgctga agtttctgga aagcctatta tcgccggccc gtccggtacc gcttcgcgca
     1021 tggtcgctgt tgcgcgtttt ctggcaccgg cttgtttgaa aagcctgggt attgagagtg
     1081 agcagaacct gaaagagctt gtgcggtatg cctgctatgc ctatttcggt caggacagcc
     1141 accattcgat gcttgaagtg aatcttggtg tcgcttccca tggaatgccg gaacaatggg
     1201 acgacacgct ttataacgag cctttcagta attcaattaa aggtcgcggg tttggtatag
     1261 acaatctcgc gcataggcaa gtcgtcaggc aggcggctca aaagtcaaag ggtgggcgcg
     1321 ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1381 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     1441 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1501 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1561 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     1621 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1681 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     1741 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1801 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1861 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     1921 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1981 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2041 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2101 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2161 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2221 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2281 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2341 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2401 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2461 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2521 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2581 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2641 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2701 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2761 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2821 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2881 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2941 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3001 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3061 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3121 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3181 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3241 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3301 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3361 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3421 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3481 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3541 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3601 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3661 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     3721 cccagtcacg acgtt
//
